YANG Chunxia,HA Jiaxin,XU Ke,et al.Research Progress on Biological Control of Colletotrichum gloeosporioides[J].Northern Horticulture,2023,(05):132-139.[doi:10.11937/bfyy.20222041]
胶孢炭疽菌生物防治研究进展
- Title:
- Research Progress on Biological Control of Colletotrichum gloeosporioides
- Keywords:
- Colletotrichum gloeosporioides; biological control; biocontrol mechanism; combination strains
- 文献标志码:
- A
- 摘要:
- 植物炭疽病是一种复杂的真菌性病害，在全球范围内均有发生，严重限制植物的种植和生产，一直以来，化学防治在植物病虫害防治中占据主导地位，由于化学防治的局限性，生物防治凭借独特的优势，迎来了良好的发展前景。基于植物炭疽病的危害和生物防治的特点，该研究以植物炭疽病主要病原菌之一胶孢炭疽菌（Colletotrichum gloeosporioides）为阐述对象，总结近年来不同微生物种类对胶孢炭疽病菌的防治进展，主要包括生防细菌、真菌、放线菌、菌种组合以及植物源的抗菌物质，并简要分析了生物防治的生防机制，最后指出当前存在的问题以及对未来发展的展望。
- Abstract:
- Plant anthracnose is a complex fungal disease that occurs all over the world and severely restricts the planting and production of plants.For a long time，chemical control has dominated the control of plant diseases and insect pests.Due to the limitations of chemical control，biological with its unique advantages，prevention and control has ushered in a good development prospect.Based on the damage of plant anthracnose and the characteristics of biological control，this study took one of the main pathogenic bacteria of plant anthracnose，Colletotrichum gloeosporioides，as the elaboration object，and summarized the control progress of different microbial species against Colletotrichum gloeosporioides in recent years.It mainly included biocontrol bacteria，fungi，actinomycetes，combination strains and plant-derived antibacterial substances，and briefly analyzed the biocontrol mechanism of biocontrol，and finally pointed out the current problems and prospects for future development.
参考文献/References:
[1]DEAN R，van KAN J A L，PRETORIUS Z A，et al.The top 10 fungal pathogens in molecular plant pathology[J].Molecular Plant Pathology,2012,13(4)：414-430.[2]刘丽萍,高洁,李玉.植物炭疽菌属Colletotrichum真菌研究进展[J].菌物研究，2020,18(4):266-281.[3]徐小文,许秀环,刘星,等.核桃炭疽病的研究进展[J].湖北农业科学,2020,59(S1):16-19.[4]韩长志.胶孢炭疽病菌的研究进展[J].华北农学报，2012，27(S1):386-389.[5]CONG J，LEI C，CHENG S，et al.Evaluating national ecological risk of agricultural pesticides from 2004 to 2017 in China[J].Environmental Pollution,2020,259(C):113778.[6]SHUQIN J，FANG Z.Zero growth of chemical fertilizer and pesticide use：China′s objectives，progress and challenges[J].Journal of Resources and Ecology,2018，9(1)：50-58.[7]蔡奕.我国植物病害生物防治的现状及发展策略[J].科技视界,2018(20):149-150.[8]于美钰.农业病虫害防治现状与方法[J].南方农业,2021,15(35):30-32.[9]将伟.生物农药的应用与研究进展:生防菌及其相关的生物农药[J].绿色科技,2019(22):203-205.[10]KIM Y S，LEE Y，CHEON W，et al.Characterization of Bacillus velezensis AK-0 as a biocontrol agent against apple bitter rot caused by Colletotrichum gloeosporioides[J].Scientific Reports,2021,11(1):626.[11]李潞滨,庄彩云,李术娜,等.兰花炭疽病拮抗细菌8-59菌株的分离与鉴定[J].河北农业大学学报,2008,31(3):64-68.[12]李佳佳,李昭煜,漆永红,等.枸杞炭疽病生防菌株筛选、生物功能测定及防治效果[J].植物保护学报,2020,47(6):1343-1352.[13]CHOUB V，AJUNA H B，WON S J，et al.Antifungal activity of Bacillus velezensis CE 100 against anthracnose disease (Colletotrichum gloeosporioides) and growth promotion of walnut (Juglans regia L.) trees[J].International Journal of Molecular Sciences,2021,22(19):10438.[14]冯江鹏,邱莉萍,梁秀燕,等.草莓胶孢炭疽菌拮抗细菌贝莱斯芽孢杆菌JK3的鉴定及其抗菌活性[J].浙江农业学报,2020,32(5):831-839.[15]王雨,谭峥,韦丹丹,等.芽胞杆菌HN-2的鉴定及抑菌活性研究[J/OL].中国生物防治学报,(2022-05-17)[2022-05-05].http://kns.cnki.net/kcms/detail/11.3515.S.20200219.1754.003.html.[16]赵德庆.芒果采后炭疽病拮抗菌的筛选、作用机制及抑菌代谢产物分析[D].海口:海南大学,2019.[17]蒋凯丽,周新丽,高海燕.一株具有拮抗作用的解淀粉芽孢杆菌的筛选、鉴定及生物学特性研究[J].工业微生物,2020,50(1):8-13.[18]汪玉玲,宋希强,郭向阳,等.文心兰炭疽病生防潜力菌的鉴定及其抑菌性的测定[J].热带生物学报,2021,12(2):236-243.[19]KEJELA T，THAKKAR V R，THAKOR P.Bacillus species (BT42) isolated from Coffea arabica L.rhizosphere antagonizes Colletotrichum gloeosporioides and Fusarium oxysporum and also exhibits multiple plant growth promoting activity[J].BMC Microbiology,2016,16(1):227.[20]孙磊,邵红,刘莹,等.春兰内生拮抗细菌gt19-1的鉴定及对辣椒胶孢炭疽菌的防效[J].安徽农业科学,2012,40(12):7145-7146,7154.[21]汪茜,胡春锦,黄思良,等.生防细菌1505的鉴定及其对采后柑橘炭疽病的抑制效果[J].西南农业学报,2011,24(2):579-585.[22]徐睿,刘闯,张潇月,等.2株拮抗生防菌对油茶炭疽病多种病原菌的抑菌活性及防效[J].经济林研究,2021,39(2):27-34.[23]杨娜,顾欣,杨环羽,等.灵武长枣炭疽病拮抗细菌J6-N的分离鉴定与生物学特性[J].宁夏大学学报(自然科学版),2020,41(3):272-278.[24]钱鑫磊,周珊珊,张冬乐,等.梨树胶孢炭疽病生防菌筛选及其抑菌活性初步评价[J].生物学通报,2017,52(12):50-53.[25]吕纪涛,魏莉,曹晓璐,等.胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析[J].河南农业科学,2015,44(7):90-93.[26]MAMY J N R，YI W,GHULAM Y D,et al.Volatile organic compounds of Bacillus atrophaeus HAB-5 inhibit the growth of Colletotrichum gloeosporioides[J].Pesticide Biochemistry and Physiology,2019,156:170-176.[27]GUERRERO-BARAJAS C，CONSTANTINO-SALINAS E A，AMORA-LAZCANO E，et al.Bacillus mycoides A1 and Bacillus tequilensis A3 inhibit the growth of a member of the phytopathogen Colletotrichum gloeosporioides species complex in avocado[J].Journal of the Science of Food and Agriculture,2020,100(10)：4049-4056.[28]汪茜.生防细菌T132的鉴定及其对采后柑橘炭疽病的抑制效果[J].微生物学通报，2012，39(9):1260-1271.[29]陈泽历,杨林毅,陈潞,等.金毛狗脊内生细菌分离鉴定及其拮抗作用分析[J].西南林业大学学报(自然科学版),2020,40(1):176-180.[30]REYES-PEREZ J J，HERNANDEZ-MONTIEL L G，VERO S，et al.Postharvest biocontrol of Colletotrichum gloeosporioides on mango using the marine bacterium Stenotrophomonas rhizophila and its possible mechanisms of action[J].Journal of Food Science and Technology,2019,56(11)：4992-4999.[31]李婷,黄蕙,吴春先,等.微生物农药登记现状简析[J].四川农业科技,2021(6):42-44.[32]SHARMA A D，SHARMA I M，SHARMA M，et al.Effectiveness of fungal，bacterial and yeast antagonists for management of mango anthracnose (Colletotrichum gloeosporioides)[J].Egyptian Journal of Biological Pest Control,2021,31(1):135.[33]张静雅,李欣雨,张成,等.木薯炭疽病拮抗木霉菌筛选与室内防效研究[J].中国生物防治学报,2022,38(1):115-124.[34]SHARMA H，RAI A K，CHETTRI R，et al.Bioactivites of Penicillium citrinum isolated from a medicinal plant Swertia chirayita[J].Archives of Microbiology,2021,203(8):5173-5182.[35]BIAN J Y，FANG Y L，SONG Q，et al.The fungal endophyte Epicoccum dendrobii as a potential biocontrol agent against Colletotrichum gloeosporioides[J].Phytopathology，2021,111(2):293-303.[36]胡丽杰,闫思远,李嘉泓,等.枸杞内生真菌对胶孢炭疽菌Colletotrichum gloeosporioides的拮抗作用及生防潜力[J].植物保护,2020,46(1):125-133.[37]刘艳明,黄雅丽,李婷,等.野生与人工栽培铁皮石斛内生真菌生物多样性比较及抗炭疽病活性菌株的筛选[J].华南农业大学学报,2020,41(3):47-55.[38]王亚红,李永丽,常乐,等.对三种苹果病原菌具抑制作用的新疆野苹果内生真菌的分离与鉴定[J].果树学报,2020,37(3):390-396.[39]LIU Z，DU S，REN Y,et al.Biocontrol ability of killer yeasts (Saccharomyces cerevisiae) isolated from wine against Colletotrichum gloeosporioides on grape[J].Journal of Basic Microbiology，2017,58(1)：60-67.[40]赵鲁宁,周秋阳,杨慧慧,等.季也蒙毕赤酵母Y35-1菌株对枇杷采后炭疽病的抑菌效果及保鲜作用[J].食品科学,2019,40(4):170-177.[41]王润菡,井敏敏,赵红,等.采后芒果炭疽病拮抗酵母菌的分离和筛选[J].食品研究与开发,2016,37(7):180-183.[42]封明,刘文杰,苏誉,等.植物内生放线菌多样性及其活性代谢产物的研究进展[J].微生物学杂志,2021,41(4):64-73.[43]SAWAI B，POONSUK P.Suppression of rice sheath blight disease using a heat stable culture filtrate from Streptomyces philanthi RM-1-138[J].Crop Protection,2014,61:1-10.[44]BOUKAEW S，CHEIRSILP B，PRASERTSAN P，et al.Antifungal effect of volatile organic compounds produced by Streptomyces salmonis PSRDC-09 against anthracnose pathogen Colletotrichum gloeosporioides PSU-03 in postharvest chili fruit[J].Journal of Applied Microbiology,2021,131(3):1452-1463.[45]张雨阳,魏有海,郭良芝,等.黄三素链霉菌15-6发酵液的抑菌活性研究[J].湖南农业科学,2021(9):25-28,41.[46]姚锦爱,黄鹏,陈峰,等.建兰炭疽病拮抗放线菌的筛选及防治效果[J].中国生物防治学报,2019,35(5):805-812.[47]辜柳霜,张月凤,张凯,等.胶孢炭疽菌生防放线菌SD-29的鉴定及抗菌活性评价[J].微生物学通报,2019(4):770-779.[48]蔡甜星,刘楚非,蔡烈伟,等.放线菌V17的分类鉴定及其对茶树炭疽病菌的拮抗作用[J].宁德师范学院学报(自然科学版),2021,33(3):281-285.[49]AHKAMI A H，ALLEN WHITE R，HANDAKUMBURA P P，et al.Rhizosphere engineering：Enhancing sustainable plant ecosystem productivity[J].Rhizosphere,2017（3）：233-243.[50]SHARMA A，SHARMA I M，SHARMA M，et al.Effectiveness of fungal，bacterial and yeast antagonists for management of mango anthracnose (Colletotrichum gloeosporioides)[J].Egyptian Journal of Biological Pest Control,2021,31(1):135.[51]李梦娇.铁皮石斛常见真菌病害的鉴定及复合菌剂的应用研究[D].昆明:云南农业大学,2017.[52]ZHANG J，YAN L T，YUAN E L，et al.Antifungal activity of compounds extracted from Cortex Pseudolaricis against Colletotrichum gloeosporioides[J].Journal of Agricultural and Food Chemistry,2014,62(21)：4905-4910.[53]KHAN M R，CHONHENCHOB V，HUANG C，et al.Antifungal activity of propyl disulfide from Neem (Azadirachta indica) in vapor and agar diffusion assays against anthracnose pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango fruit[J].Microorganisms 2021，9(4)：839.[54]YE H，WANG Q，ZHU F，et al.Antifungal activity of alpha-mangostin against Colletotrichum gloeosporioides in vitro and in vivo[J].Molecules,2020,25(22):5335.[55]JANTHONG P，SUWANPHAKDEE C，LEEPASERT T，et al.Bioefficacy of piper extracts in the management of chilli anthracnose[J].Archives of Phytopathology and Plant Protection，2021(7):1-13.[56]原小迪,张思颖,李钰,等.玉米病害生防菌剂研究现状[J].安徽农业科学,2022,50(5):1-4.[57]CHEN O，YI L，DENG L L，et al.Screening antagonistic yeasts against citrus green mold and the possible biocontrol mechanisms of Pichia galeiformis (BAF03)[J].Journal of the Science of Food and Agriculture,2020,100(10):3812-3821.[58]刘海洋,姚举,张仁福,等.甲基营养型芽胞杆菌AL7对棉花黄萎病的盆栽防治效果及定殖能力[J].中国生物防治学报,2017,33(3):378-384.[59]蒋继志,张聪颖,梁娇,等.拮抗菌HT-6对致病疫霉的竞争及诱导性抑制[J].河北大学学报(自然科学版),2021,41(5):572-580.[60]MONIKA J，RITIKA P，SATYAWATI S，et al.Biocontrol mechanisms of Bacillus sp.，isolated from tomato rhizosphere，against Fusarium oxysporum f.sp.lycopersici[J].Biological Control,2018,123：60-70.[61]杨倩.侧孢短芽孢杆菌S62-9产抗菌物质的分离纯化及部分特性的研究[D].保定:河北农业大学,2010.[62]郭蔓,张朝正,赵华.贝莱斯芽孢杆菌抑尖孢镰刀菌脂肽类物质的鉴定[J].中国酿造,2021,40(12):35-39.[63]于地美.萎缩芽孢杆菌XW2胞外蛋白的纯化鉴定及其抑菌机制研究[D].北京:北京林业大学,2019.[64]ALAMOUDI S.The importance of isolated and purified Bacillus species as a source of a variety of antimicrobial substances[J].Journal of Pharmaceutical Research International，2020,32(33):94-103.[65]杨萍,杨谦,许倩.棘孢木霉生物防治相关代谢产物研究[J].哈尔滨商业大学学报(自然科学版),2014,30(1):36-40.[66]林成伟,刘金龙.木霉菌株对几种病原真菌的生物防治作用研究[J].植物医生,2021,34(2):29-33.[67]邱德文.我国植物病害生物防治的现状及发展策略[J].植物保护,2010,36(4):15-18,35.
相似文献/References:
[1]鲁海菊,王 波,潘柳君,等.深绿木霉P3.9生防菌株固体发酵条件优化筛选[J].北方园艺,2014,38(14):119.
LU Hai-ju,WANG Bo,PAN Liu-jun,et al.Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride [J].Northern Horticulture,2014,38(05):119.
[2]熊仕俊,孙成龙,施闯,等.番茄青枯病菌拮抗放线菌的筛选及鉴定[J].北方园艺,2014,38(05):114.
XIONG Shi-jun,SUN Cheng-long,SHI Chuang,et al.Screening and Identifying of Antagonistic Actinomycetes Against Ralstonia solancearum[J].Northern Horticulture,2014,38(05):114.
[3]高 宇.茶尺蠖天敌生态学研究进展[J].北方园艺,2014,38(09):203.
GAO Yu.Research Progress on Ecology of Natural Enemies of Tea Geometrid[J].Northern Horticulture,2014,38(05):203.
[4]全桂静.苹果采后病害拮抗细菌的筛选与防腐效果研究[J].北方园艺,2013,37(11):130.
QUAN Gui-jing.Study on Screening of Antagonistic Bacteria for Apple Postharvest Diseases and Biocontrol Efficacy[J].Northern Horticulture,2013,37(05):130.
[5]欧善生,韦荣昌,吴庆华,等.广西凉粉草病虫害种类及防治方法研究[J].北方园艺,2013,37(01):177.
OU Shan-sheng,WEI Rong-chang,WU Qing-hua,et al.Study on Varieties of Diseases and Insect Pests and Control Measures of Mesona chinensis in Guangxi[J].Northern Horticulture,2013,37(05):177.
[6]穆瑞瑞.土壤微生物在农村植物病害生物防治中的作用分析[J].北方园艺,2013,37(08):194.
MU Rui-rui.Analysis of Soil Microorganism on Biological Control of Plant Diseases in Rural Areas[J].Northern Horticulture,2013,37(05):194.
[7]胡琼,张海松.木霉菌TC对辣椒疫病的防治效果[J].北方园艺,2012,36(03):147.
HU Qiong,ZHANG Hai-song.Effect of Biological Control of Pepper Phytophthora Blight by Trichoderma TC[J].Northern Horticulture,2012,36(05):147.
[8]葛平华,马桂珍,付泓润,等.油菜菌核病的生物防治研究进展[J].北方园艺,2013,37(02):185.
GE Ping-hua,MA Gui-zhen,FU Hong-run,et al.Research Progress of the Biological Control of Sclerotinia sclerotiorum[J].Northern Horticulture,2013,37(05):185.
[9]古丽吉米拉·米吉提,王志英,王 娜,等.木霉菌的生物防治分子机理[J].北方园艺,2013,37(23):206.
MIJITI Gulijimila,WANG Zhi-ying,WANG Na,et al.Molecular Mechanism of Biological Control of Trichoderma spp.[J].Northern Horticulture,2013,37(05):206.
[10]李 阳,宋素琴,王 静,等.苹果树腐烂病的防治研究进展[J].北方园艺,2015,39(13):194.[doi:10.11937/bfyy.201513054]
LI Yang,SONG Suqin,WANG Jing,et al.Research Progress on Control of the Valsa Cancer of Apple Trees[J].Northern Horticulture,2015,39(05):194.[doi:10.11937/bfyy.201513054]
备注/Memo
第一作者简介：杨春霞（1997-），女，硕士研究生，研究方向为枸杞炭疽病生防菌筛选和复合菌剂开发。E-mail：1147378529@qq.com.责任作者：刘成(1973-)，男，硕士，高级实验师，现主要从事微生物菌剂功能等研究工作。E-mail：liu0cheng@126.com.基金项目：宁夏自然科学基金资助项目（2021AAC03160）；宁夏自然科学基金重点研发资助项目（2020BFG02006）。收稿日期：2022-05-17