WANG Yilian,YANG Kai,CHEN Kai,et al.Conditioner Combined With Microbial Agents for the Remediation of Continuous Cropping Soil and Yield of Brassica chinensis L.[J].Northern Horticulture,2022,(20):84-91.[doi:10.11937/bfyy.20221234]
调理剂联合微生物菌剂对小油菜连作土壤修复及产量的影响
- Title:
- Conditioner Combined With Microbial Agents for the Remediation of Continuous Cropping Soil and Yield of Brassica chinensis L.
- Keywords:
- conditioner; Trichoderma spp.; Bacillus atrophicus; continuous cropping obstacle; soil remediation
- 文献标志码:
- A
- 摘要:
- 以“黑叶五月慢青菜”小油菜为试材，采用小区试验的方法，研究了调理剂联合微生物菌剂对小油菜连作障碍土壤理化性质、酶活及产量的影响，探索复合修复对土壤连作障碍的缓解作用，以期为叶菜的安全生产提供参考依据。结果表明：调理剂（7 500 kg·hm-2）能有效调理油菜连作土壤的酸碱度，能将pH≤4.50的极强酸性土壤改良至中性，同时降低土壤EC值，提高速效磷含量，降低碱解氮和速效钾含量，增加土壤中Ca2+、Na+、Mg2+、Cl-、HCO-3含量，降低K+、SO2-4和NO-3含量，提高S-SC、S-NR、S-CAT和S-CL活性；添加微生物菌剂能促进有机质和全氮、全磷、全钾的循环，提高小油菜净菜产量，667 m2增产率8.40%～33.59%；木霉LTR-2能显著降低土壤中的NO-3含量，提高产量，菌剂贡献率为4.57%，农学利用率为13.34 kg·kg-1；复合修复机理是调理剂调节土壤pH，微生物菌剂LTR-2降低土壤中的NO-3含量，提高土壤酶活性，促进有机质和全氮、全磷、全钾的循环，改善小油菜的生长环境，提高产量。
- Abstract:
- Taking ‘Heiyewuyueman Vegetables’ edible rape as test material,a plot experiment was carried out to study the effects of conditioner and microbial agent on soil physical and chemical properties,enzyme activities and yield of rape,the aim of this study was to explore the mitigation effect of complex remediation on soil continuous cropping obstacles and in order to provide reference for the safety of leaf vegetables production.The results showed that the dosage of 7 500 kg·hm-2conditioner could effectively regulate the pH of rape continuous cropping soil and improve the extremely acidic soil (pH4.50) to neutral.Meanwhile,the soil conductivity was decreased,the content of available phosphorus was increased,but alkali-hydrolyzed nitrogen and available potassium decreased.In addition,the contents of Ca2+,Na+,Mg2+,Cl- and HCO-3 in soil were increased,the contents of K+,SO2-4 and NO-3 were decreased,and the soil saccharase,nitrate reductase,catalase and cellulose activities were increased.The addition of microbial agents was beneficial to the recycling of soil organic matter and total nitrogen,total phosphorus and total potassium in rape continuous cropping,and the yield of rape was increased to varying degrees,with the yield increasing rate ranging from 8.40% to 33.59%.Compared with the control,Trichoderma LTR-2 significantly reduced NO-3 content in soil and increased the yield of small rape,the contribution rate of fungus was 4.57%,and the agronomic utilization rate was 13.34 kg·kg-1.The mechanism of composite remediation was that soil conditioner adjusted soil pH,microbial agent LTR-2 reduced NO-3 content in soil,improved soil enzyme activity and recycling of organic matter,total nitrogen,total phosphorus and total potassium to improve the growth environment of rapeseed and increase yield.
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备注/Memo
第一作者简介：王贻莲（1978-），女，硕士，副研究员，现主要从事设施蔬菜田退化土壤的生物修复等研究工作。E-mail：yilianwang@163.com.责任作者：李纪顺（1971-），男，本科，研究员，现主要从事农田退化土壤的修复等研究工作。E-mail：yewu2@sdas.org.基金项目：齐鲁工业大学（山东省科学院）产学研协同创新基金资助项目（2020-CXY11）；威海市政府-山东省科学院产学研协同创新基金资助项目（2020GC05）。收稿日期：2022-03-30