KANG Yuqian,LI Yuxin,ZHANG Tingting,et al.Color Phenotype and Pigment Identification of Bracts of Bougainvillea×buttiana ‘Chitra’[J].Northern Horticulture,2022,(21):66-73.[doi:10.11937/bfyy.20221209]
‘画报’三角梅苞片色彩表型及色素种类鉴定
- Title:
- Color Phenotype and Pigment Identification of Bracts of Bougainvillea×buttiana ‘Chitra’
- Keywords:
- Bougainvillea×buttiana; bracts; color; pigment
- 文献标志码:
- A
- 摘要:
- 以‘画报’三角梅为试材，使用比色卡及色差仪测量‘画报’不同类型苞片的色彩表型，并对其进行聚类分析；使用紫外-可见光谱法定性测量‘画报’苞片中所含色素类型，后针对所含色素进一步进行定量分析，研究‘画报’三角梅的色素组成，以期为其它观赏物种植物的色彩鉴定和色素分析提供参考依据。结果表明：色彩表型鉴定表明‘画报’三角梅7种类型的苞片可以分为绿色系和红色系两大色系。深绿色苞片、浅绿色苞片、绿心红边苞片绿色部分、绿心粉边绿色部分以及白色苞片归于绿色系，深红色苞片、粉红色苞片、绿心红边红色部分、绿心粉边粉色部分归于红色系；紫外光谱表明‘画报’苞片中含有叶绿素、类黄酮、类胡萝卜素以及甜菜色素，但是不含有类黄酮色素中的花青素。色素含量测量结果依次为类黄酮＞叶绿素＞甜菜色素＞类胡萝卜素。综上所述，‘画报’三角梅苞片的颜色呈现主要依靠类黄酮色素，该研究结果可为进一步研究三角梅苞片呈色机理奠定基础。
- Abstract:
- The color phenotypes of different bracts of Bougainvillea×buttiana ‘Chitra’ were measured by RHSCC and colorimeter,and their clustering analysis was carried out.The pigment types in the bracts of ‘Chitra’ were qualitatively measured by ultraviolet-visible spectrophotometer,and then the pigment types were further quantitatively analyzed,the pigment composition of ‘Chitra’ was studied,in order to provide reference for color identification and pigment analysis of other species of ornamental plants.The results showed that the bracts of seven types of ‘Chitra’ could be divided into green and red two color systems.The dark green bracts,light green bracts,green part of bracts with red margin,green part of bracts with pink margin bracts and white bracts belonged to the green system,and the dark red bracts,pink bracts,red part of bracts with green heart,pink part of bracts with green heart belonged to the red system.Ultraviolet spectrum showed that the bracts of ‘Chitra’ contained chlorophyll,flavonoids,carotenoids and betalains,but did not contain anthocyanins.The results of pigment content were as follows,flavonoid>chlorophyll>beet pigment>carotenoid.In conclusion,the color presentation of ‘Chitra’ bracts mainly depended on flavonoids pigments.The results of this study laid a foundation for further study on the color mechanism of bougainvillea bracts.
参考文献/References:
[1]周群,黄克福,丁印龙,等.中国引栽三角梅属观赏品种的调查与分类鉴定[J].江西农业学报,2011,23(5):53-56.[2]陈香波,罗玉兰,张启翔.三角梅在我国的温度适宜分布区划[J].中国园林,2009,25(7):97-99.[3]ALI S M,SHARMIM S,YOUNUS I,et al.Antidepressant and inhibitory effect on MAO isoenzymes by Bougainvillea glabra flower extract in rats[J].Pak J Pharm Sci,2021,34(5):1963-1968.[4]熊亚,李敏杰.三角梅植株不同部位水提取液抑菌效果研究[J].北方园艺,2016(9):129-132.[5]CHANG S X,LI C X,JIANG Y Z,et al.Characteristics of the pollen morphology and viability of Bougainvillea (Nyctaginaceae)[J].Scientia Horticulturae,2021,277:109732.[6]徐夙侠,黄青云,林春松,等.不同育性三角梅品种的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究（英文）[J].植物研究,2018,38(6):860-868.[7]ANURADHA,GREWAL H S.Effect of growth regulators on morphological development and rooting of stem cuttings of bougainvillea[J].Agricultural Research Journal,2018,55(3):581-585.[8]常圣鑫,黄素荣,徐世松,等.三角梅扦插枝化学诱变育种[J].热带作物学报,2019,40(2):238-246.[9]周群.三角梅新品种‘香妃1号’[J].园艺学报,2021,48(S2):2991-2992.[10]马若晨,乔鑫,刘秀丽.4个三角梅品种的耐寒性评价[J].分子植物育种,2021,19(2):687-696.[11]曾莹,山金凤,祝遵凌,等.不同三角梅品种苞片颜色变化规律[J].北方园艺,2018(23):79-86.[12]张慧会,曾莹,圣倩倩,等.不同三角梅品种苞片色素成分分析[J/OL].分子植物育种，（2021-05-19)[2022-03-04].https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=FZZW20210518006&uniplatform=NZKPT&v=_MB1PS_Ch2Adn2HUuMFM0Q5mlYScE1HvuTP U1WyliD8YoHwKppuTOMoa3wqMzjrY.[13]姜卫兵,庄猛,韩浩章,等.彩叶植物呈色机理及光合特性研究进展[J].园艺学报,2005,32(2):352-358.[14]徐夙侠,黄青云,林春松,等.甜菜色素的生物学研究进展[J].亚热带植物科学,2019,48(4):378-383.[15]张磊,曹德美,胡建军.植物叶色形成调控机制研究进展[J].植物遗传资源学报,2021,22(2):293-303.[16]再吐娜·买买提,许建,姚军,等.甜瓜果实发育过程中叶绿素和类胡萝卜素含量的变化[J].中国瓜菜,2021,34(8):44-48.[17]CHEN C,ZHOU G,CHEN J,et al.Integrated metabolome and transcriptome analysis unveils novel pathway involved in the formation of yellow peel in cucumber[J].Int J Mol Sci，2021,22(3):1494.[18]ZHOU Z,GAO H,MING J,et al.Combined transcriptome and metabolome analysis of pitaya fruit unveiled the mechanisms underlying peel and pulp color formation[J].BMC Genomics，2020,21(1):734.[19]黄欢,胡慧霞,姜联合,等.植物甜菜色素的研究进展[J].生物学通报,2014,49(8):1-4,64.[20]赵昶灵,郭维明,陈俊愉.梅花花色色素种类和含量的初步研究[J].北京林业大学学报,2004(2):68-73.[21]孙世萍.高效液相色谱法检测甜菜红色素的方法研究[D].石河子:石河子大学,2006.[22]陈建,吕长平,陈晨甜,等.不同花色非洲菊品种花色素成分初步分析[J].湖南农业大学学报(自然科学版),2009,35(S1):73-76.[23]赵春丽,陈家兰,王乐,等.温度对苋菜幼苗中甜菜色素含量及其相关基因表达的影响[J].应用与环境生物学报,2020,26(6):1484-1489.[24]郭鑫,成仿云,钟原,等.紫斑牡丹花色表型数量分类研究[J].园艺学报,2022,49(1):86-99.[25]杜习武,叶康,秦俊,等.玉兰品种花色表型数量分类研究[J].种子,2021,40(11):68-73,2.[26]殷涵泰,尹俊梅,廖易,等.基于秋石斛花朵颜色、色素分布及表皮细胞形态的表型分类[J].园艺学报,2021,48(10):1907-1920.[27]沈星诚,周婷,范俊俊,等.日本红枫春季叶片色彩评价[J].南京林业大学学报(自然科学版),2020,44(6):213-220.[28]TANAKA Y,BRUGLIREA F,CHANDLER S.Recent progress of flower colour modification by biotechnology[J].Int J Mol Sci,2009,10(12):5350-69.[29]刘安成,尉倩,王峰伟,等.几种忍冬属植物开花过程中花色和色素的变化[J].西南大学学报(自然科学版),2020,42(1):22-29.[30]钟培星,王亮生,李珊珊,等.芍药开花过程中花色和色素的变化[J].园艺学报,2012,39(11):2271-2282.[31]张学明,齐晓光,陈玉波,等.草莓类黄酮化合物的研究进展[J].北方园艺,2020(1):128-133.[32]林启芳,刘婷婷,刘洁茹,等.紫薇属与黄薇属植物花瓣类黄酮组成及含量分析[J].园艺学报,2021,48(10):1956-1968.[33]陈倩如,蔡文淇,张霞,等.檵木不同叶色形成的化学成分比较研究[J].园艺学报,2021,48(10):1969-1982.[34]YI D,ZHANG H,LAI B,et al.Integrative analysis of the coloring mechanism of red Longan pericarp through metabolome and transcriptome analyses[J].J Agric Food Chem,2021,69(6):1806-1815.[35]ZHANG L,CHEN C,XIE F,et al.A novel WRKY transcription factor HmoWRKY40 associated with betalain biosynthesis in Pitaya (Hylocereus monacanthus) through regulating [STBX]HmoCYP76AD1[STBZ][J].Int J Mol Sci,2021,22(4):2171.[36]许丽颖,王庆芬,张俊晟,等.九种不同类型彩叶植物色素含量的季节性变化研究[J].北方园艺,2013(6):59-62.[37]MARKESTEIJN L,POORTER L,PAZ H,et al.Ecological differentiation in xylem cavitation resistance is associated with stem and leaf structural traits[J].Plant Cell Environ,2011,34(1):137-148.[38]PPLTURAK G,BREITRLl D,GROSSMAN N.Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants[J].New Phytol,2016,210(1):269-283.[39]GAO S M,LIU Y,LU J,et al.Analysis of 407 nm light induced erythrocyte fluorescence spectra red shifted mechanism with the concentration increase[J].光子学报,2003,32(3):261-263.[40]MOHAMMAD I K,GIRIDHAR P.Plant betalains:Chemistry and biochemistry[J].Phytochemistry,2015,117:267-295.
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备注/Memo
第一作者简介：康宇乾（1996-），男，硕士研究生，研究方向为观赏植物品质性状改良。E-mail：kangyuqian@hainanu.edu.cn.责任作者：周扬（1988-），男，博士，副教授，现主要从事植物抗逆分子生物学等研究工作。E-mail：zhouyang@hainanu.edu.cn.基金项目：海南省耐盐作物生物技术重点实验室资助项目（2021）（HD-SYSZX-202107）；海南大学科研启动资助项目(KYQD（ZR）1845)。收稿日期：2022-03-29