DU Juan,CHEN Zixin,XIE Chunyan.Extraction of Flavonoids From Walnut Leaves and Its Antioxidant Activity[J].Northern Horticulture,2022,(19):94-99.[doi:10.11937/bfyy.20220943]
核桃叶的黄酮类物质提取及其抗氧化活性
- Title:
- Extraction of Flavonoids From Walnut Leaves and Its Antioxidant Activity
- Keywords:
- flavonoids; ethanol; reflux extraction; anti oxidation
- 文献标志码:
- A
- 摘要:
- 以核桃叶为试材，采用乙醇回流提取法提取核桃叶中的黄酮类物质，研究了提取时间、提取温度、液料比以及乙醇浓度对核桃叶中黄酮提取得率的影响，并对提取条件进行优化，以期为核桃叶中黄酮的开发利用提供参考依据。结果表明：采用乙醇回流提取方式从核桃叶中提出黄酮的最佳条件为提取时间3 h，提取温度60 ℃，液料比50∶1 mL·g-1，乙醇浓度80%，黄酮提取量最高值为20.50 mg·g-1。黄酮提取液对DPPH自由基清除率和总抗氧化能力虽然不如维生素C，但也具有较强的抗氧化能力。
- Abstract:
- Taking walnut leaf as test material, the effects of extraction time,extraction temperature,liquid-solid ratio and ethanol concentration on the extraction rate of flavonoids in walnut leaf were studied by using ethanol reflux extraction method,and the extraction conditions were optimized,in order to provide reference for the development and utilization of flavonoids in walnut leaf.The results showed that the optimum conditions for extracting flavonoids from walnut leaves by ethanol reflux extraction were extraction time 3 hours,extraction temperature 60 ℃,liquid-solid ratio 50∶1 mL·g-1,ethanol concentration 80%,and the maximum amount of flavonoids extracted was 20.50 mg · g-1.The flavonoids extract had a stronger antioxidant capacity,although its scavenging rate of DPPH free radicals and total antioxidant capacity were not as good as those of vitamin C.
参考文献/References:
[1]蒲光兰,肖千文,赖腾跃,等.核桃砧木、穗条内含物及其与嫁接成活率的关系[J].陕西师范大学学报(自然科学版),2016,44(2):79-84.[2]赵见军,王丁丁,张亮,等.我国核桃综合利用与发展前景[J].陕西农业科学,2014,60(4):56-59,73.[3]姚欣,让凤菊,刘伟,等.伊犁野核桃叶中黄酮类活性物质的提取及其抑菌活性研究[J].伊犁师范学院学报(自然科学版),2018,12(3):31-35.[4]陈红红.山核桃叶总黄酮的提取分离与抗氧化作用的研究[D].合肥:安徽农业大学,2008.[5]吴肖虎.几种黄酮类化合物的抗氧化性研究[D].桂林:广西师范学院,2014.[6]萨茹丽.沙葱黄酮提取工艺优化、结构鉴定及其相关生物活性研究[D].呼和浩特:内蒙古农业大学,2014.[7]刘丹.芹菜黄酮类化合物的提取纯化及其抗氧化性研究[D].上海:华东理工大学,2014.[8]金钟.沙棘叶黄酮提取物体内外抗氧化活性、应用与护肝作用的研究[D].哈尔滨:东北农业大学,2014.[9]刘思宏,高琦,铁梅,等.果蔬皮渣活性物质提取研究进展[J].粮食与油脂,2018,31(4):20-23.[10]周坚,叶甫云.水提法提取银杏中种皮中黄酮类物质的研究[J].武汉工业学院学报,1999(3):1-6.[11]韩立杰,李影,刘子菱,等.榛花粉水和乙醇提取物抗氧化性的比较[J].食品科技,2014,39(2):224-226.[12]张羽飞,于春丽,张春枝.乙醇室温浸提法提取黄芩总黄酮的工艺条件[J].大连轻工业学院学报,2004(3):190-193.[13]溥丽华,刘忠华,魏振园,等.乙醇浸提法提取槐角愈伤组织中异黄酮的研究[J].种子,2012,31(1):1-4.[14]李志美.核桃的开发利用[J].林业调查规划,2004(S1):199-201.[15]翟梅枝.植物次生物质的抗病活性及构效分析[D].福州:福建农林大学,2003.[16]方菊.竹叶黄酮的提取分离及抑菌效果研究[D].合肥:合肥工业大学,2012.[17]田君,池汝安,张越非,等.从土茯苓浸取液萃取黄酮的研究[J].解放军药学学报,2006(5):340-342.[18]刘江,周荣琪.竹叶提取物总黄酮含量测定方法的改进[J].食品科技,2005(7):76-79.[19]郭芳.原花青素及其脂质体在化妆品中的应用研究[D].上海:东华大学,2015.[20]马岚.银杏叶中黄酮的提取与分离纯化研究[D].西安:长安大学,2015.
相似文献/References:
[1]毕舒童,周 清,李国铭,等.乙醇浓度及处理时间对土壤消毒效果的影响[J].北方园艺,2014,38(13):172.
BI Shu-tong,ZHOU Qing,LI Guo-ming,et al.Effect of the Concentration of Ethanol Solutions and Processing Time on Fusarium oxysporum?and Phytophthora capsici in Biological Soil Disinfection Using Ethano[J].Northern Horticulture,2014,38(19):172.
[2]赵娟娟.响应面法优化提取菠菜中黄酮类物质的工艺研究[J].北方园艺,2013,37(14):137.
ZHAO Juan-juan.Study on Optimization Extraction Conditions of Flavonoid from Spinach by RSM[J].Northern Horticulture,2013,37(19):137.
[3]石美,贾旭,张航航,等.转色期乙醇处理对葡萄成熟特性与着色进程的影响[J].北方园艺,2019,43(09):111.[doi:10.11937/bfyy.20183409]
SHI Mei,JIA Xu,ZHANG Hanghang,et al.Effect of Preharvest EtOH Treament on Ripening Properties and Coloration Process of Grape[J].Northern Horticulture,2019,43(19):111.[doi:10.11937/bfyy.20183409]
[4]李响,熊亚男,靳亚忠,等.乙烯、脱落酸以及乙醇对采后薄皮甜瓜果实软化及调节酶活性的影响[J].北方园艺,2021,(21):100.[doi:10.11937/bfyy.20211333]
LI Xiang,XIONG Yanan,JIN Yazhong,et al.Effects of ETH,ABA and EtOH on Softening and Regulatory Enzyme Activities in Postharvest Oriental Melon[J].Northern Horticulture,2021,(19):100.[doi:10.11937/bfyy.20211333]
[5]孙浩轩,王佳江,李岩,等.壳聚糖和乙醇复合处理对薄皮甜瓜采后贮藏品质的影响[J].北方园艺,2023,(14):105.[doi:10.11937/bfyy.20223719]
SUN Haoxuan,WANG Jiajiang,LI Yan,et al.Effects of Chitosan and Ethanol Compound Treatments on Postharvest Storage Quality of Thin skinned Muskmelon[J].Northern Horticulture,2023,(19):105.[doi:10.11937/bfyy.20223719]
备注/Memo
第一作者简介：杜娟（1983-），女，硕士，副研究员，现主要从事农业科学等研究工作。E-mail：dujuan0203@163.com.责任作者：解春艳（1983-），女，博士，教授，现主要从事食品科学等研究工作。E-mail：xcy8046@163.com.基金项目：河北省科技计划资助项目（19227133D）。收稿日期：2022-03-15