ZHANG Jianhong,HONG Chuntao,SHEN Dengfeng,et al.Effects of Salt Stress on Antioxidant Enzyme Activity of Pecan (Carya illinoensis K.Koch) Seedlings[J].Northern Horticulture,2022,(18):23-28.[doi:10.11937/bfyy.20220589]
盐胁迫对薄壳山核桃幼苗抗氧化酶活性的影响
- Title:
- Effects of Salt Stress on Antioxidant Enzyme Activity of Pecan (Carya illinoensis K.Koch) Seedlings
- Keywords:
- pecan; survival rate; salt stress; antioxidant enzyme; Na+/K+
- 文献标志码:
- A
- 摘要:
- 以一年生薄壳山核桃‘Pawnee’的容器苗为试材，采用不同浓度的NaCl溶液0%（CK）、0.3%、0.4%、0.5%、0.6%、0.7%对其进行盐胁迫处理，确定耐受浓度。再以薄壳山核桃‘Pawnee’幼苗为试材，采用不同NaCl浓度水平（0%、0.3%、0.6%和0.9%）胁迫处理，研究了盐胁迫对其物候期、叶片抗氧化酶活性和Na+/K+的影响,以期为薄壳山核桃栽培提供参考依据。结果表明：在0.3%NaCl处理后薄壳山核桃落叶时间比对照提早了26 d，且存活率与对照一致，均为97.1%；而盐浓度达到0.7%NaCl处理后植株的存活率为49.5%；NaCl浓度水平的升高显著增加‘Pawnee’幼苗叶片中Na+/K+和脯氨酸（Pro）含量；另外，丙二醛（MDA）含量和超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性在处理前期的10 d或30 d，随NaCl浓度水平增大而增加；但在盐处理后期，尤其在高NaCl浓度水平（0.6%和0.9%）下，其活性随着胁迫处理时间的增加呈下降趋势。
- Abstract:
- The container seedlings of one-year-old Carya illinoensis ‘Pawnee’ were used as test materials,and NaCl solutions of different concentrations 0% (CK),0.3%,0.4%,0.5%,0.6%,0.7% were used to treat them with salt stress to determine the tolerance concentration,and treated with different NaCl concentrations (0%,0.3%,0.6% and 0.9%) to study the effects of salt stress on its phenological period,leaf antioxidant enzyme activities and the Na+/K+,in order to provide reference for planting Carya illinoensis.The results showed that after the 0.3% NaCl treatment,the deciduous time of the pecan was 26 days earlier than that of the control,the survival rate was 97.1%,which was the same as that of the control;and the survival rate of the plants after the salt concentration reached 0.7% NaCl was 49.5%.In addition,the Na+/K+ and proline (Pro) content in leaves of ‘Pawnee’ seedlings significantly increased with the increase of NaCl concentration level;in addition,the content of malondialdehyde (MDA) and superoxide dismutase activity (SOD),peroxidase activity (POD),and catalase activity (CAT) increased with the increase of NaCl concentration in the first 10 days or 30 days of treatment;but in the later stage of salt treatment,especially,at high NaCl concentration for the levels of 0.6% and 0.9%,its activity showed a downward trend with the increase of stress treatment time.
参考文献/References:
[1]TA B K，TA B F，ABDERRAHIM L A，et al.Effect of salt stress on growth,chlorophyll content,lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L.[J].South African Journal of Botany,2016，105：306-312.[2]OUERGHI Z，CORNIC G,ROUDANI M，et al.Effect of NaCl on photosynthesis of two wheat species (Triticum durum and T.aestivum) differing in their sensitivity to salt stress[J].Journal of Plant Physiology，2000，156(3)：335-340.[3]MORADI F,ISMAIL A M.Responses of photosynthesis,chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice[J].Annals of Botany，2007，99(6)：1161-1173.[4]HICHEM H，NACEUR E A,MOUNIR D.Effects of salt stress on photosynthesis,PSⅡ photochemistry and thermal energy dissipation in leaves of two corn (Zea mays L.) varieties[J].Photosynthetica，2009，47(4)：517-526.[5]DEMIRAL T，RKAN I.Comparative lipid peroxidation,antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance[J].Environmental and Experimental Botany，2005，53(3)：247-257.[6]YANG Y Q，GUO Y.Elucidating the molecular mechanisms mediating plant salt-stress responses[J].New Phytologist，2018，217(2)：523-539.[7]ISAYENKOV S V，MAATHUIS F J.Plant salinity stress:Many unanswered questions remain[J].Frontiers in Plant Science，2019（10）：1-11.[8]谢孝福，黄婉芳.长山核桃选优研究初报[J].中国果树,1991(1)：3.[9]杨敏文，葛明菊，马国芳.微波消解-火焰光度法测定叶片中钾和钠含量[J].光谱试验室，2002，19(6)：800-803.[10]唐启义.DPS统计软件简介[J].中国医院统计，2008，15(2)：1.[11]MATTIOLI R，COSTANTINO P,TROVATO M.Proline accumulation in plants:Not only stress[J].Plant Signaling & Behavior，2009，4(11)：1016-1018.[12]赵靖明,孙凡,姚小华,等.NaCl胁迫对薄壳山核桃幼苗生长及光合生理特性的影响[J].西南师范大学学报（自然科学版）,2012,37(12):93-97.[13]GILL S S，TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010，48(12)：909-930.[14]PITZSCHKE A，FORZANI C，HIRT H.Reactive oxygen species signaling in plants[J].Antioxidants & Redox Signaling，2006，8(9/10)：1757-1764.[15]WANG W，VINOCUR B，ALTMAN A.Plant responses to drought,salinity and extreme temperatures:Towards genetic engineering for stress tolerance[J].Planta,2003，218(1)：1-14.[16]YANG Y Q，GUO Y.Elucidating the molecular mechanisms mediating plant salt-stress responses[J].New Phytologist，2018，217(2)：523-539.[17]ZHANG H，HAN B，WANG T，et al.Mechanisms of plant salt response:Insights from proteomics[J].Journal of Proteome Research，2012，11(1)：49-67.[18]CHAI Y Y，JIANG C D，SHI L，et al.Effects of exogenous spermine on sweet sorghum during germination under salinity[J].Biologia Plantarum,2010，54(1):145-148.[19]DAT J，van DENABEELE S，VRANOVA E，et al.Dual action of the active oxygen species during plant stress responses[J].Cellular and Molecular Life Sciences CMLS，2000，57(5):779-795.[20]SHABALA S，SCHIMANSKI L J,KOUTOULIS A.Heterogeneity in bean leaf mesophyll tissue and ion flux profiles:Leaf electrophysiological characteristics correlate with the anatomical structure[J].Annals of Botany,2002，89(2):221-226.[21]赵春梅，崔继哲，金荣荣.盐胁迫下植物体内保持高K+/Na+比率的机制[J].东北农业大学学报，2012，43(7)：155-160.
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备注/Memo
第一作者简介：章建红(1976-),男，博士，正高级工程师，现主要从事资源植物等研究工作。E-mail:nbjianhong@163.com.责任作者：潘存德（1964-），男，博士，教授，现主要从事经济林与森林生态等研究工作。E-mail:pancunde@163.com.基金项目：宁波市科技富民资助项目（2015C10029）；浙江省重点研发专项资助项目（2018C02004）。收稿日期：2022-02-21