WEN Die,WU Xin,HUANG Qianhui,et al.Identification and Experssion Patterns Analysis of Expansin [STBX]fvexpl2[STBZ] in Flammulina filiformis[J].Northern Horticulture,2020,44(12):135-141.[doi:10.11937/bfyy.20194542]
金针菇类扩张蛋白[STBX]fvexpl2[STBZ]鉴定及其表达模式分析
- Title:
- Identification and Experssion Patterns Analysis of Expansin [STBX]fvexpl2[STBZ] in Flammulina filiformis
- Keywords:
- expansin; morphogenesis; stipe elongation; evolutionary tree; real-time quantitative fluroescence PCR
- 文献标志码:
- A
- 摘要:
- 基于金针菇基因组与转录组数据，利用在线软件BLAST鉴定了一个扩张蛋白类似物编码基因，命名为[STBX]fvexpl2[STBZ]，并对其基因结构进行了鉴定，通过构建进化树的方法分析了[STBX]fvexpl2[STBZ]与担子菌、子囊菌扩张蛋白类似物以及植物扩张蛋白之间的亲缘关系。结果表明：扩张蛋白类似物外显子的数目为4个，内含子的数目为3个，并且CDS的长度是438 bp，分子量为15 746.88 Da，等电点为4.93；进化树图显示[STBX]fvexpl2[STBZ]与担子菌类扩张蛋白处于同一亚支，亲缘关系较近。实时荧光定量PCR结果则表明[STBX]fvexpl2[STBZ]在菌柄伸长部位具有较高的表达水平，其表达水平远远高于菌盖、原基以及菌丝部位的表达水平，且其在伸长较快部位表达水平显著高于伸长较慢部位表达水平，所以[STBX]fvexpl2[STBZ]可能对菌柄伸长起重要作用。
- Abstract:
- Based on the genome and transcriptome data of Flammulina filiformis，this experiment identified an expansin analog encoding gene using online software BLAST.It was named [STBX]fvexpl2[STBZ] and identified its gene structure.The phylogenetic relationship between [STBX]fvexpl2[STBZ] and basidiomycetes，ascomycetes and plants was analyzed by constructing evolutionary tree.The results showed that four exons and three introns contained in the dilatant protein analogue，and the length of coding sequence was 438 bp,molecular weight 15 746.88 Da,isoelectric point 4.93.The construction of evolutionary tree showed that [STBX]fvexpl2[STBZ] and basidiomycetes were in the same subclan and had close relationship.According to the real-time fluorescence quantitative PCR results,we could understand that [STBX]fvexpl2[STBZ] was much higher than that in the cap，primordium and mycelium,and it had a higher expression level in the fast elongation region of the stipe compared with the slow elongation region.Therefore，[STBX]fvexpl2[STBZ] might play an important role in the elongation of the stipe.
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备注/Memo
第一作者简介：温蝶（1993-），女，硕士研究生，研究方向为食（药）用菌。E-mail:webdie@126.com.责任作者：温志强（1965-），男，本科，教授，现主要从事食（药）用菌安全生产和病虫害防治等研究工作。E-mail:595154360@qq.com.基金项目：福建省农业厅种业创新与产业化工程资助项目(fjzycxny2017010)。收稿日期：2020-03-14