LIU Nannan,WANG Guiqing.Isolation and Identification of Pathogenic Fungi From Red Fruit Ailanthus altissima Seedling Disease[J].Northern Horticulture,2019,43(17):89-94.[doi:10.11937/bfyy.20184580]
红果臭椿苗期病害致病菌的分离与鉴定
- Title:
- Isolation and Identification of Pathogenic Fungi From Red Fruit Ailanthus altissima Seedling Disease
- Keywords:
- red fruit Ailanthus altissima; seeding disease; pathogenic fungi; separation and identification
- 文献标志码:
- A
- 摘要:
- 以感病红果臭椿幼苗病株为试材，采用形态学鉴定、分子生物学鉴定和致病性鉴定方法，研究了引起红果臭椿苗期病害致病菌的种类，以期为其苗期病害防治提供了有力的依据，从而提高红果臭椿的成活率，为园林绿化提供充足的苗源。结果表明：红果臭椿苗期病害有5种致病真菌，分别为木贼镰刀菌（Fusarium equiseti）、腐皮镰刀菌（F．solani）、极细链格孢菌（Alternaria tenuissima）、球毛壳菌（Chaetomium globosum）和新棒孢拟盘多毛孢菌（Neopestalotiopsis clavispora），其中腐皮镰刀菌为主要致病菌。
- Abstract:
- The susceptibility of red fruit Ailanthus altissima as a variety of A.altissima，seedlings was used as a test material.Morphological identification，molecular biological identification and pathogenicity identification methods were used to study the types of pathogenic fungi causing red fruit A.altissima seedling disease.In order to provide a strong basis to improve the survival rate and prevention control of red fruit skunk,so as to provide sufficient seedling source for landscaping.The results showed that there were five pathogenic fungi in the seedling stage of red fruit A.altissima，namely：Fusarium equiseti,F.solani,Alternaria tenuissima，and Chaetomium globosum and Neopetalotiopsis clavispora，of which F.solani was the main pathogen.
参考文献/References:
[1]王洪鸣,苏德喜,石菊香,等.红果臭椿培育试验[J].宁夏农林科技,2009(2):42-43.[2]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.[3]王桂清,曾路,马迪,等.国槐根茎腐烂病病原菌的形态与分子鉴定[J].东北林业大学学报,2017,45(5):106-110.[4]方中达.植病研究方法[M].北京:中国农业出版社，1984.[5]BORRERO C,CASTA〖AKN~〗O R,AVILS M.First Report of Pestalotiopsis clavispora (Neopestalotiopsis clavispora) causing canker and twig dieback on blueberry bushes in Spain[J].Plant disease,2017,102(6):1178.[6]徐卉,张秀省,穆红梅,等.臭椿开发应用研究进展[J].北方园艺,2014(11):181-183.[7]刘文军,赵娜.臭椿生长习性及三种常见病害的防治[J].城乡园林,2018(4):166.[8]王宴荷.臭椿常见病虫害及防治技术[J].林业科技,2017(14):81-82.[9]杨宇红,杨翠荣,凌键,等.设施蔬菜苗期病害病原鉴定及化学药剂筛选[J].中国蔬菜,2015(6):28-34.[10]穆敏,何党军,毕锦暇.花生苗期病害研究[J].陕西农业科学,2010(2):104-105.[11]李文兵,白进玲,王颖.我国华北区甜菜苗期未知叶斑病害的诊断鉴定[J].中国糖料,2018,40(5):13-15.[12]王玉.菜豆树叶斑病病原学及药剂敏感性研究[D].长春:吉林农业大学,2017.[13]马婉琴,蔡苏,钱永生,等.球毛壳菌引起杭白菊叶枯病的首次报道[J].杭州师范大学学报（自然科学版）,2015,14(4):390-393.[14]梁海林,童志武,朱笃.球毛壳菌次级代谢产物及其生物活性研究进展[J].天然产物研究与开发,2018,30(4):702-707.
相似文献/References:
[1]李进,李杰,段俊杰,等.不同种衣剂对番茄苗期两种病害的防效及其产量的影响[J].北方园艺,2017,41(09):111.[doi:10.11937/bfyy.201709024]
LI Jin,LI Jie,DUAN Junjie,et al.Effect of Different Seed Coating on Control Disease and Yield at Seedling Stage of Tomato[J].Northern Horticulture,2017,41(17):111.[doi:10.11937/bfyy.201709024]
备注/Memo
第一作者简介：刘南南（1991-），女，山东聊城人，硕士研究生，研究方向为园林植物栽培与保护。E-mail：1150122446@qq.com.责任作者：王桂清（1968-），女，河北泊头人，博士，教授，现主要从事植物保护的教学与科研等工作。E-mail：wangguiqing@lcu.edu.cn.基金项目：山东省重点研发计划资助项目（2014GNC110020）；山东省聊城市科技发展计划资助项目（2014GJH10）。收稿日期：2019-01-23