ZHANG Wei,LIU Di,YU Tingting,et al.Study on the Difference of Organic Acid Components in Normal ‘Pingguoli’ Pear and Its Large-fruited Mutant at Different Developmental Stages[J].Northern Horticulture,2019,43(12):14-19.[doi:10.11937/bfyy.20183760]
普通型和大果芽变型苹果梨不同发育时期果实有机酸组分差异性
- Title:
- Study on the Difference of Organic Acid Components in Normal ‘Pingguoli’ Pear and Its Large-fruited Mutant at Different Developmental Stages
- Keywords:
- ‘Pingguoli’; bud mutant; fruit; organic acid; citrate acid; malic acid
- 文献标志码:
- A
- 摘要:
- 为明确苹果梨普通型和大果型芽变果实有机酸品质形成的差异及其产生的原因，以苹果梨及其大果型芽变果实为试材，采用高效液相色谱法（HPLC）测定了果实不同发育时期（细胞分裂期、果实迅速生长期和果实成熟期）中的柠檬酸、苹果酸、琥珀酸、奎宁酸和莽草酸含量。结果表明：1）苹果梨果实主要有机酸为苹果酸和柠檬酸，可分为柠檬酸型果实和苹果酸型果实，普通型苹果梨为柠檬酸型果实，大果型芽变果实为苹果酸型果实。2）无论是普通型苹果梨还是大果型芽变苹果梨在细胞分裂期均不产生柠檬酸；二者在细胞分裂期时奎宁酸含量最高；芽变型苹果梨其苹果酸含量在成熟期最高，普通型苹果梨其柠檬酸含量在成熟期最高；普通型苹果梨及芽变型苹果梨莽草酸含量均在细胞分裂期最高。
- Abstract:
- In order to clarify the difference in the formation of organic acid quality between normal ‘Pingguoli’ pear and its large-fruited mutant and the causes.Normal ‘Pingguoli’ pear and its large-fruited mutant were used as test materials.The contents of citric acid，malic acid，succinic acid，quinic acid and shikimic acid in different developmental stages of the fruit (cell division，rapid growth of fruit and fruit ripening)determined by high performance liquid chromatography (HPLC).The results showed that,1) The main organic acids of ‘Pingguoli’ pear were malic acid and citric acid，which could be divided into citric acid type and malic acid type fruit.‘Pingguoli’ pear was citric acid type fruit，large-fruited mutant of ‘Pingguoli’ pear became malic acid type fruit.No citric acid was produced in either ‘Pingguoli’ pear or its large-fruited mutant during cell division.Both of them had the highest content of quinic acid during cell division;the content of malic acid in the large-fruited mutant of ‘Pingguoli’ pear was the highest in the mature stage，and the content of citric acid in ‘Pingguoli’ pear was the highest in the mature stage;the shikimic acid contents of ‘Pingguoli’ pear and its large-fruited mutant were the highest in cell division period.
参考文献/References:
[1]CHEN F X,LIU X H,CHEN L S.Advances in research on organic acid metabolism in fruits[J].Journal of Fruit Science,2005,22(5);526-531.[2]胡志群,王惠聪,胡桂兵.高效液相色谱测定荔枝果肉中的糖、酸和维生素C[J].果树学报,2005(5):582-585.[3]梁俊,郭燕,刘玉莲,等.不同品种苹果果实中糖酸组成与含量分析[J].西北农林科技大学学报(自然科学版),2011,39(10):163-170.[4]BARTOLOZZI F,BERTAZZA G,BASSI D,et al.Simultaneous determination of soluble sugars and organic acids as their trimethylsilyl derivatives in apricot fruits by gas-liquid chromatography[J].Journal of Chromatography A,1997,758(1):99-107.[5]周元,傅虹飞.猕猴桃中的有机酸高效液相色谱法分析[J].食品研究与开发,2013,34(19):85-87.[6]胡红菊,王友平,陈启亮,等.不同熟期砂梨生长及主要糖酸含量动态的研究[J].湖北农业科学,2007(4):637-640.[7]姚改芳,杨志军,张绍铃,等.梨不同栽培种果实有机酸组分及含量特征分析[J].园艺学报,2014,41(4):755-764.[8]岳英,鲁晓燕,谢成龙,等.不同品种(品系)梨汁中有机酸含量分析[J].新疆农业科学,2012,49(10):1881-1886.[9]姚改芳.不同栽培种梨果实糖酸含量特征及形成规律研究[D].南京:南京农业大学,2011.[10]霍月青,胡红菊,彭抒昂,等.砂梨品种资源有机酸含量及发育期变化[J].中国农业科学,2009,42(1):216-223.[11]张树军.‘南果梨’大果型芽变的细胞、生理及分子基础研究[D].南京:南京农业大学,2011.[12]何志刚,李维新,林晓姿,等.枇杷果实成熟和贮藏过程中有机酸的代谢[J].果树学报,2005,22(1):23-26.[13]LAMIKANRA O,INYANG I D,LEONG S.Distribution and effect of grape maturity on organic acid content of red muscadine grapes[J].Journal of Agricultural and Food Chemistry,1995,43(12):3026-3028.[14]张秀梅,杜丽清,孙光明,等.菠萝果实发育过程中有机酸含量及相关代谢酶活性的[J].果树学报,2007(3):381-384.[15]SADKA A,ARTZI B,COHEN L,et al.Arsenite reduces acid content in citrus fruit，inhibits activity of citrate synthase but induces its gene expression[J].Journal of the American Society for Horticultural Science,2000,125:288-293.[16]WU B H,QUILOT B,GENARD M,et al.Changes in sugar and organic acid concentrations maturation in peaches,P,davidiana and hybrids as analyzed by principal component during fruit analysis[J].Scientia Horticul,2005,103:42-39.[17]NAGY S,SHAW P E.Tropical and subtropical fruits:Composition,properties and uses[M].Westport,Connecticut:AVI Publishing，Inc.,1980.[18]陈发兴,刘星辉,陈立松.枇杷果肉有机酸组分及有机酸在果实内的分布[J].热带亚热带植物学报,2008(3):236-243.[19]潘瑞炽.植物生理学[M].2版.北京:高等教育出版社,1995:337.[20]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990(6):55-57.[21]陈发兴,刘星辉,陈立松.果实有机酸代谢研究进展[J].果树学报,2005,22(5):526-531.[22]刘建军,姜鲁燕,赵祥颖,等.L-苹果酸的应用及研究进展[J].中国食品添加剂,2003(3):53-56，52.[23]沙守峰.梨有机酸组分及含量变化与遗传鉴定[D].南京:南京农业大学,2012.
相似文献/References:
[1]王旭,曲柏宏,刘振坤.延边苹果梨苯丙氨酸解氨酶基因克隆及植物表达载体的构建[J].北方园艺,2013,37(07):106.
WANG Xu,QU Bai-hong,LIU Zhen-kun.Clone and Construction of Plant Expression Vector of Phenylalanine Ammonia Lyase Gene of Pyrus pyrifolia cv. Pingguoli in Yanbian Area[J].Northern Horticulture,2013,37(12):106.
[2]王鹏,丛明亮,权跃,等.两种壁蜂在苹果梨园上的访花特性研究[J].北方园艺,2012,36(07):19.
WANG Peng,CONG Ming-liang,QUAN Yue,et al.Study on Two Kinds of Osmia’s Behavior of Flower-visiting in Apple-pear Orchard[J].Northern Horticulture,2012,36(12):19.
[3]何建华,邵建柱,郭玉,等.早熟芽变“嘎拉”与普通“嘎拉”苹果的生物学性状比较[J].北方园艺,2012,36(14):20.
HE Jian-hua,SHAO Jian-zhu,GUO Yu,et al.Comparison of Biological Characteristics Between ‘Gala’ Apple and Its Early Maturing Bud Mutation[J].Northern Horticulture,2012,36(12):20.
[4]杨宏霞,曲柏宏,刘振坤,等.延边苹果梨PyDFR基因的克隆及表达分析[J].北方园艺,2015,39(04):99.[doi:10.11937/bfyy.201504023]
YANG Hong-xia,QU Bai-hong,LIU Zhen-kun,et al.Molecular Cloning and Expression Analysis on PyDFR Gene of Pingguoli in Yanbian[J].Northern Horticulture,2015,39(12):99.[doi:10.11937/bfyy.201504023]
[5]王 家 珍,李 俊 才,刘 成,等.苹果梨杂种后代亲本性状遗传倾向的研究[J].北方园艺,2010,34(09):0.[doi:10.11937/bfyy.201009008]
W ANG Jia -zhen,LI Jun -cai,LIU Cheng,et al.Study on Parental T raits Genetic Predisposition of Apple -pear Hybrid Offspring[J].Northern Horticulture,2010,34(12):0.[doi:10.11937/bfyy.201009008]
[6]王雪,曲柏宏,鹿艳新,等.苹果梨果皮花色素苷合成相关基因PyANS的克隆与表达分析[J].北方园艺,2016,40(05):108.[doi:10.11937/bfyy.201605030]
WANG Xue,QU Baihong,LU Yanxin,et al.Molecular Cloning and Expression of PyANS in ‘Pingguoli’ During the Pigmentation[J].Northern Horticulture,2016,40(12):108.[doi:10.11937/bfyy.201605030]
[7]张俊苗,李文胜,史进.短枝条红型“红富士”苹果芽变光合特性的日变化研究[J].北方园艺,2016,40(06):24.[doi:10.11937/bfyy.201606006]
Study on Diurnal Variations of Photosynthetic Characteristics of a New Spur and Stripe Red Fuji Apple.Study on Diurnal Variations of Photosynthetic Characteristics of a New Spur and Stripe ‘Red Fuji’ Apple[J].Northern Horticulture,2016,40(12):24.[doi:10.11937/bfyy.201606006]
[8]李 含 芬,马 春 晖,尹 晓 宁.苹果梨柱头数目对种子和果实发育的影响[J].北方园艺,2009,33(05):0.[doi:10.11937/bfyy.200905022]
LI Han - fen,MA Chun- hui,YIN Xiao - ning.Effects of Stigma on the Development of Seeds and Fruit of Pear Cultivar `Pingguoli’[J].Northern Horticulture,2009,33(12):0.[doi:10.11937/bfyy.200905022]
[9]卢 立 华,武 泽 民,宋 海 森,等.苹 果 梨 树 环 剥 效 应 试 验[J].北方园艺,2009,33(08):0.[doi:10.11937/bfyy.200908058]
[J].Northern Horticulture,2009,33(12):0.[doi:10.11937/bfyy.200908058]
[10]庄 得 凤,曹 后 男,宗 成 文,等.苹 果 梨 优 良 授 粉 品 种 的 选 择[J].北方园艺,2008,32(01):0.[doi:10.11937/bfyy.200801009]
ZHUANG De -feng,CAO Hou-nan,ZONG Chen -wen,et al.The Choice of Excellent Pollinated Varieties in Pingguoli[J].Northern Horticulture,2008,32(12):0.[doi:10.11937/bfyy.200801009]
备注/Memo
第一作者简介：张微(1995-)，女，硕士研究生，研究方向为果树发育生物学。E-mail：zwybu234@163.com.责任作者：刘迪(1973-)，男，博士，副教授，现主要从事果树及菌物发育生物学等研究工作。E-mail：liudi@ybu.edu.cn.基金项目：吉林省教育厅“十三五”科学技术资助项目(JJKH20170462KJ)；吉林省科技厅自然科学基金资助项目(20170101022JC)。收稿日期：2018-11-26