[1]连青龙,田婧,鲁少尉,等.菊花乙烯响应因子CmRAP2-12的克隆与表达特性分析[J].北方园艺,2018,42(17):89-96.[doi:10.11937/bfyy.20180365]
　LIAN Qinglong,TIAN Jing,LU Shaowei,et al.Cloning,Characterization and Expression Analysis of ERF CmRAP2-12 in Chrysanthemum morifolium[J].Northern Horticulture,2018,42(17):89-96.[doi:10.11937/bfyy.20180365]
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菊花乙烯响应因子CmRAP2-12的克隆与表达特性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年17 页码: 89-96 栏目: 出版日期: 2018-09-10
Title:
Cloning,Characterization and Expression Analysis of ERF CmRAP2-12 in Chrysanthemum morifolium
作者:
连青龙1; 2; 田婧1; 2; 鲁少尉1; 2; 马宁1; 2; 鲍顺淑1; 2; 李邵1; 2
（1.农业部规划设计研究院 设施农业研究所，北京 100125；2.农业部农业设施结构工程重点实验室，北京 100125）
Author(s):
LIAN Qinglong1; 2; TIAN Jing1; 2; LU Shaowei1; 2; MA Ning1; 2; BAO Shunshu1; 2; LI Shao1; 2
(1.Institute of Facility Agriculture,Chinese Academy of Agricultural Engineering,Beijing 100125；2.Key Laboratory of Farm Building in Structure and Construction,Ministry of Agriculture,Beijing 100125)
关键词:
菊花; 乙烯转录因子; 淹水胁迫; 低氧; 表达
Keywords:
Chrysanthemum morifolium; ethylene response factor(ERF); waterlogging stress; hypoxic; expression
DOI:
10.11937/bfyy.20180365
文献标志码:
A
摘要:
以菊花(Chrysanthemum morifolium)品种‘神马’为试材，采用RT-PCR与RACE、Real-time RT-PC等技术，克隆了菊花乙烯转录因子CmRAP2-12基因全长的cDNA序列片段，Real-time RT-PCR分析了水淹胁迫对菊花CmRAP2-12基因的表达模式的影响，以期降低水淹条件下对菊花生长的迫害性影响。结果表明：得到的CmRAP2-12的cDNA序列为1 335 bp；CmRAP2-12保守氨基酸的N末端含有一个具有Ⅶ ERFs 亚家族特征的氨基酸序列，且聚类到RAP2-12亚家族一类，并预测亚细胞定位在细胞核中。Real-time RT-PCR分析显示，CmRAP2-12在菊花的根、茎、叶中均有表达，并且在经过0～72 h的淹水胁迫后，CmRAP2-12基因在菊花根系中的表达量均呈现一个先升高后降低的趋势，并在处理后48 h达到最高值，淹水胁迫提高了菊花根系乙烯响应转录因子RAP2-12的表达水平。该试验将为进一步研究RAP2-12对提高菊花水淹低氧耐受性的调节机理与分子响应机制提供参考依据。
Abstract:
In this study,a full-length cDNA named CmRAP2-12 was cloned in Chrysanthemum morifolium ‘Jinba’ roots by RT-PCR and RACE,and the real time RT-PCR was used to analyze the expression pattern of the three genes.The results showed that cDNA sequence of CmRAP2-12 obtained in this study was 1 335 bp,CmRAP2-12 conserved N-terminal amino acids contained a Ⅶ ERFs subfamily characteristic amino acid sequence,and clustered to RAP2-12 subfamily. CmRAP2-12 was predicted subcellular localization in the nucleus.Real-time RT-PCR analysis showed that CmRAP2-12 was all expressed in root,stem and leaf in Chrysanthemum.CmRAP2-12 expression levels presented a first increased and then decreased trend,after waterlogging stress 0-72 hours.It was treated after 48 hours reached the highest value.The expression levels of ethylene response transcription factor 〖STBX〗RAP2-12 〖STBZ〗was increased in Chrysanthemum roots and affected by hypoxic response when suffering from waterlogging stress.This study would provide the theoretical basis for further research 〖STBX〗RAP2-12 〖STBZ〗to improve chrysanthemum flooding tolerance to hypoxia and molecular mechanisms regulating response mechanism.

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备注/Memo

第一作者简介：连青龙（1981-），男，博士，高级工程师，研究方向为设施园艺生物技术。E-mail：qinglong_lian@163.com.基金项目：国家自然科学基金资助项目（31401896，31501805）。收稿日期：2018-03-12

更新日期/Last Update: 2018-09-19