ZHANG Meihua,CUI Yue,ZHANG Shaobin.Expression Analysis of Pea Actin Isoform （PEAc3） Under Different Drought Conditions[J].Northern Horticulture,2017,41(08):107-110.[doi:10.11937/bfyy.201708024]
豌豆肌动蛋白异型体PEAc3在不同干旱条件下的表达分析
- Title:
- Expression Analysis of Pea Actin Isoform （PEAc3） Under Different Drought Conditions
- 关键词:
- 豌豆; 肌动蛋白异型体; 实时荧光定量RT-PCR
- 文献标志码:
- A
- 摘要:
- 以豌豆幼苗为试材，采用半定量和实时荧光定量RT-PCR技术，研究了不同浓度PEG 6000对肌动蛋白异型体PEAc3表达的影响，为深入了解豌豆幼苗抗旱分子机理以及筛选耐旱豌豆品种奠定基础。结果表明：与正常供水处理相比，15% PEG 6000处理下PEAc3基因的表达量略有升高，20% PEG 6000处理下PEAc3基因的表达量显著升高，低于10% PEG 6000处理，PEAc3的表达量基本没有变化，而25% PEG 6000处理下PEAc3基因的表达量则有所下降。不同干旱条件下PEAc3基因表达量不同这一结果表明，PEAc3基因可能参与豌豆幼苗适应干旱胁迫的生理生化过程，该基因有可能用于豌豆抗旱性强弱的分子筛选。
- Abstract:
- Pea seedlings were used as experimental materials，and the expression of PEAc3 was studied under different concentrations of PEG 6000 by using semi quantitative RT-PCR and real-time fluorescence quantitative RT-PCR technique，which made a good foundation for understanding the molecular mechanism of drought resistance in pea seedlings and select pea cultivars of drought tolerant.The results showed that compared with the normal water supply，the expression of PEAc3 gene was significantly increased under 20% PEG 6000 and a slight increase under 15% PEG 6000，and was not changed below 10% PEG 6000.However，the expression of PEAc3 gene was decreased under 25% PEG 6000.The expression of PEAc3 gene in different drought conditions showed that the PEAc3 gene might be involved in the physiological and biochemical process of the pea seedling adaptation to drought stress，and the gene might be used for the molecular screening of the drought resistance of pea.
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备注/Memo
第一作者简介：张美华（1990-），女，河南商丘人，硕士研究生，研究方向为植物生物化学与分子生物学。E-mail：601849776@qq.com.责任作者：张少斌（1973-），男，辽宁彰武人，博士，副教授，现主要从事蛋白质工程与作物生物技术等研究工作。E-mail：zsb010024@163.com.基金项目：辽宁省自然科学基金资助项目（2013020072）。