[1]徐卫平,刘凤娟,毕春晓,等.大岩桐SsPIN2基因的克隆及组织表达分析[J].北方园艺,2016,40(24):88-91.[doi:10.11937/bfyy.201624022]
　XU Weiping,LIU Fengjuan,BI Chunxiao,et al.Cloning of SsPIN2 Gene From Sinningia speciosa and Its Expression in Different Tissues[J].Northern Horticulture,2016,40(24):88-91.[doi:10.11937/bfyy.201624022]

点击复制

大岩桐SsPIN2基因的克隆及组织表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷期数: 2016年24 页码: 88-91 栏目: 出版日期: 2016-12-30

Title:: Cloning of SsPIN2 Gene From Sinningia speciosa and Its Expression in Different Tissues

作者:: 徐卫平1; 刘凤娟2; 毕春晓2; 胡鑫2; 徐全乐2; 蒋景龙1; （1.陕西理工大学生物科学与工程学院，陕西汉中 723001；2.西北农林科技大学生命科学学院，陕西杨凌 712100）

Author(s):: XU Weiping1; LIU Fengjuan2; BI Chunxiao2; HU Xin2; XU Quanle2; JIANG Jinglong1; (1.School of Biological Science and Engineering，Shaanxi Sci-tech University，Hanzhong，Shaanxi 723001；2.College of Life Sciences，Northwest A&F University，Yangling，Shaanxi 712100)

关键词:: 大岩桐; SsPIN2; 基因克隆; 表达分析

Keywords:: Sinningia speciosa; SsPIN2 gene; gene cloning; expression analysis

DOI:: 10.11937/bfyy.201624022

文献标志码:: A

摘要:: 以大岩桐为试材，采用RT-PCR方法对大岩桐SsPIN2基因进行了克隆，获得898 bp的基因序列，经BLAST比对，采用邻接法构建进化树；并利用半定量RT-PCR方法，研究了SsPIN2基因在大岩桐叶、茎尖、根、茎、花瓣和花萼组织的表达情况。结果表明：大岩桐SsPIN2基因与黄瓜CsPIN2、豌豆PsPIN2基因等亲缘关系较近；该基因在上述6种组织中均有表达组织，其中在茎尖、根、茎和花萼的表达量较高，在叶和花瓣中的表达量较低。

Abstract:: Sinningia speciosa were used as materials，and 898 bp cDNA in length of SsPIN2 gene was isolated.BLAST and phylogenetic trees via neighbor-joining (NJ) method were used to analysis sequence.And semi-quantitative RT-PCR was used to investigate the different expression level of SsPIN2 gene in different tissues of leaf，shoot tips，root，stem，petal and calyx.The results indicated that SsPIN2 shared a high sequence identity with CsPIN2 and PsPIN2. SsPIN2 was expressed in all of the above tissues and relatively higher in shoot tips，root，stem and calyx and relatively lower in leaf and petal.

参考文献/References:

[1]徐全乐,谢亚红,刘文婷,等.大岩桐高频再生体系建立的两种途径[J].园艺学报,2010,37(1):135-140.

[2]徐全乐.大岩桐组织培养研究进展[J].北方园艺,2011(23):166-170.

[3]庞基良,王利琳,胡江琴,等.重瓣紫蓝大岩桐组培苗的花同源异型现象[J].实验生物学报,2003,36(1):76-81.

[4]XU Q L,HU Z,LI C Y,et al.Tissue culture of Sinningia speciosa and analysis of the in vitro-generated tricussate whorled phyllotaxis (twp) variant[J].In Vitro Cell and Developmental Biology Plant,2009,45:583-590.

[5]胡鑫,徐全乐.连续组织培养引起大岩桐再生植株的表型变化[J].西北农业学报,2010,19(5):167-170.

[6]REINHARDT D.Phyllotaxis:a new chapter in an old tale about beauty and magic numbers[J].Curr Opin Plant Biol,2005,8:487-493.

[7]徐全乐,胡鑫.植物叶序的发生和影响因素[J].植物生理学通讯,2009,45(4):405-412.

[8]REINHARDT D,PESCE E R,STIEGER P,et al.Regulation of phyllotaxis by polar auxin transport[J].Nature,2003,426(6964):255-260.

[9]GUENOT B,BAYER E,KIERZKOWSKI D,et al.PIN1-independent leaf initiation in Arabidopsis[J].Plant Physiol,2012,159(4):1501-1510.

[10]CHEN Y,FAN X,SONG W,et al.Over-expression of OsPIN2 leads to increased tiller numbers，angle and short plant height through suppression of OsLAZY1[J].Plant Biotechnol J,2012,10(2):139-149.

[11]BENJAMINS R,QUINT A,WEIJERS D,et al.The PINOID protein kinase regulates organ development in Arabidopsis by enhancing polar auxin transport[J].Development,2001,128:4057-4067.

[12]LEE BH,JOHNSTON R,YANG Y,et al.Studies of aberrant phyllotaxy1mutants of maize indicate complex interactions between auxin and cytokinin signaling in the shoot apical meristem[J].Plant Physiol,2009,150:205-216.

[13]NAKAYAMA N,SMITH R S,MANDEL T,et al.Mechanical Regulationof Auxin-Mediated Growth[J].Curr Biol,2012,22:1468-1476.

[14]XU Q L,DONG J L,GAO N,et al.Transgenic lines of Begonia maculata generated by ectopic expression of PttKN1[J].Biologia,2011,66(2):251-257.

[15]XU Q L,RUAN M Y,HU X.Ectopic expression of the PttKN1 gene in coleus (Solenostemon scutellarioides)[J].Indian J Genet,2013,73(4):419-425.

[16]JACKSON D,HAKE S.Control of phyllotaxis in maize by the abphyl1 gene[J].Development,1999,126:315-323.

[17]GIULINI A,WANG J,JACKSON D.Control of phyllotaxy by thecytokinin-inducible response regulator homologue ABPHYL1[J].Nature,2004,430:1031-1034.

[18]PALAUQUI J C,LAUFS P.Phyllotaxis:In Search of the Golden Angle[J].Curr Biol,2011,21:502-504.

[19]PRASAD K,GRIGG S P,BARKOULAS M,et al.Arabidopsis PLETHORA transcription factors control phyllotaxis[J].Curr Biol,2011,13:1123-1128.

[20]ITOH J,HIBARA K,KOJIMA M,et al.Rice DECUSSATE controls phyllotaxy by affecting the cytokinin signaling pathway[J].Plant J,2012,72:869-881.

[21]YANG F,BUI H T,PAUTLER M,et al.A maize glutaredoxin gene，abphyl2，regulates shoot meristems size and phyllotaxy[J].Plant Cell,2015,27:121-131.

[22]SMITH R S,GUYOMARCH’H S,MANDEL T,et al.A plausible model of phyllotaxis[J].PNAS,2006,103:1301-1306.

相似文献/References:

[1]尹智慧,宋丽莉,司亮,等.大岩桐叶片再生体系的建立及卡那霉素敏感性研究[J].北方园艺,2013,37(10):100.
　YIN Zhi-hui,SONG Li-li,SI Liang,et al.Study on Optimization of Tissue Culture System of Sinningia speciosa Benth. and the Sensitivity on Kanamycin[J].Northern Horticulture,2013,37(24):100.
[2]郭　丽.大岩桐水分胁迫生理生化的研究[J].北方园艺,2011,35(18):0.[doi:10.11937/bfyy.2011018033]
　Ｓｔｕｄｙｏｎ　ｔｈｅ　Ｅｆｆｅｃｔ　ｏｆ　Ｄｒｏｕｇｈｔ　Ｓｔｒｅｓｓ　ｏｎ　Ｐｈｙｓｉｏｌｏｇｉｃａｌ　Ｉｎｄｅｘ　ｏｆ　Ｓｉｎｎｉｎｇｉａｓｐｅｃｉｏｓａ[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.2011018033]
[3]徐全乐.大岩桐组织培养研究进展[J].北方园艺,2011,35(23):0.[doi:10.11937/bfyy.2011023060]
　Ｒｅｓｅａｒｃｈ　Ｐｒｏｇｒｅｓｓ　ｏｎ　Ｔｉｓｓｕｅ　Ｃｕｌｔｕｒｅ　ｏｆ　Ｇｌｏｘｉｎｉａ[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.2011023060]
[4]李建民,文清成,吕守成,等.大岩桐的组培快繁及其产业化技术研究[J].北方园艺,2011,35(04):0.[doi:10.11937/bfyy.21104055]
　LI Jian-min,WEN Qing,chengI,et al.Study on Rapid Propagation and Industrial Procedure of Sinningia speciosa[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.21104055]
[5]王海洪.大岩桐组织培养及其练苗移栽技术[J].北方园艺,2008,32(04):0.[doi:10.11937/bfyy.200804092]
　[J].Northern Horticulture,2008,32(24):0.[doi:10.11937/bfyy.200804092]
[6]任如意,赵金良,宗宪春,等.大岩桐叶片组织培养及其组织学观察[J].北方园艺,2008,32(06):0.[doi:10.11937/bfyy.200806083]
　REN Ru -yi,ZHAO Jin -liang,ZONG Xian -chun,et al.Tissue Culture With Leaves of Sinningia speciosa and Histological Observation[J].Northern Horticulture,2008,32(24):0.[doi:10.11937/bfyy.200806083]
[7]刘雪莲,顾地州,高艳蕊,等.大岩桐腋芽丛生的诱导及植株再生[J].北方园艺,2008,32(07):0.[doi:10.11937/bfyy.200807093]
　LIU Xue -lian,GU Di -zhou,GAO Yan -rui,et al.Multiple Shoot Induction and Plantlet Regeneration in Sinningia speciosa[J].Northern Horticulture,2008,32(24):0.[doi:10.11937/bfyy.200807093]
[8]毕春晓,刘凤娟,曲瑞红,等.大岩桐SsKN1基因的克隆及组织表达分析[J].北方园艺,2018,42(18):69.[doi:10.11937/bfyy.20180190]
　BI Chunxiao,LIU Fengjuan,QU Ruihong,et al.Cloning of SsKN1 Gene From Sinningia speciosa and Its Expression in Different Tissues[J].Northern Horticulture,2018,42(24):69.[doi:10.11937/bfyy.20180190]

备注/Memo

第一作者简介：徐卫平（1994-），女，硕士研究生，研究方向为植物抗逆生物学。E-mail：xweiping16@163.com.责任作者：蒋景龙（1980-），男，博士，讲师，硕士生导师，研究方向为植物学。E-mail：jiangjinglong511@163.com.基金项目：国家自然科学基金资助项目（31401910）；中国博士后科学基金面上资助项目（2016M590975）；陕西省科技厅自然科学基础研究计划资助项目（2014JQ3113）；陕西省教育厅自然科学研究资助项目（14JK1158）。

更新日期/Last Update: 2017-02-13