ZHAO Bo,LI Jingjian,MAO Shizhong,et al.Application of DNA Barcoding Based on the Chloroplast ndhA Intron Region in Classification of Begonia (Begoniaceae)[J].Northern Horticulture,2016,40(16):103-107.[doi:10.11937/bfyy.201616027]
基于叶绿体ndhA基因内含子序列的DNA条形码在秋海棠属物种鉴定中的应用
- Title:
- Application of DNA Barcoding Based on the Chloroplast ndhA Intron Region in Classification of Begonia (Begoniaceae)
- Keywords:
- Begonia; ndhA intron region; DNA barcoding; species identification
- 文献标志码:
- A
- 摘要:
- 以18种秋海棠属植物为试材，基于叶绿体ndhA基因内含子片段，利用Codon Code Aligner软件拼接序列后，通过MEGA软件进行比对并计算19个秋海棠属植物种间及种内遗传距离，最后利用邻接法构建分子系统树，研究了该片段作为DNA条形码对秋海棠属植物进行物种鉴定的可行性。结果表明：秋海棠属19个种类共59个个体的ndhA基因内含子序列长度为1 182 bp，在所考察的候选秋海棠属植物中具有最大的种间变异和较小的种内变异，且二者存在极显著差异。在系统树中，秋海棠属植物每一物种能够分别形成各自独立的分支，表现出良好单系性。基于ndhA基因内含子的DNA条形码在识别秋海棠属植物物种方面和传统形态学基本一致。该研究表明，以ndhA基因内含子作为秋海棠属植物DNA条形码进行物种鉴定具有一定的可行性。
- Abstract:
- Taking 58 samples from 18 species of Begonia as materials，chloroplast ndhA intron region was used as a popular marker of DNA barcoding for species identification to evaluate its species resolution rates in the genus Begonia.The sequences of the ndhA intron region were assembled using the Codon Code Aligner，and then aligned and the genetic distances were computed using MEGA 5.0 in accordance with the kimura 2-parameter (K2P) model.The neighbor-joining (NJ) phylogenetic trees were constructed.The results showed that the ndhA intron region was about 1 182 bp in length，and exhibited the highest inter-specific divergence，and this was significantly higher than the intra-specific variation.The ndhA intron region had reasonable high identification efficiency among all tested samples.The NJ trees revealed that all individuals of each species formed a strong monophyletic group.Species identification with ndhA intron region in cluster supported the taxonomy based on morphological characters.The conclusion suggested that chloroplast ndhA intron region was a valid DNA barcoding gene for species identification in genus Begonia.
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备注/Memo
第一作者简介：赵博（1981-），女，河南南阳人，博士,副研究员，研究方向为植物细胞学与分子系统发育。E-mail：calljone@163.com.责任作者：唐文秀（1973-），女，本科，副研究员，现主要从事珍稀濒危植物保护等研究工作。E-mail:tangwx99@163.com.基金项目:广西自然科学基金资助项目（2012GXNSFBA053075）。