BAI Helong,ZHU Wenxue,WANG Jing,et al.Supercritical CO2 Fluid Extraction of the Fatty Oil From Zizyphi spinosi Semen Optimized by Response Surface Methodology[J].Northern Horticulture,2016,40(13):136-140.[doi:10.11937/bfyy.201613036]
响应面法优化SFE-CO2提取酸枣仁中脂肪油及成分分析
- Title:
- Supercritical CO2 Fluid Extraction of the Fatty Oil From Zizyphi spinosi Semen Optimized by Response Surface Methodology
- Keywords:
- Zizyphi spinosi Semen; fatty oil; supercritical CO2 fluid extraction; response surface method; GC-MS
- 文献标志码:
- A
- 摘要:
- 以酸枣仁为研究对象，采用超临界流体萃取技术提取油酸枣仁中脂肪油成分，并利用响应面分析法分析了超临界流体萃取的试验条件，研究了系统压力、提取温度、动态萃取时间和酸枣仁粉末粒度4个影响因素对脂肪油提取效果的影响，并应用GC-MS技术对脂肪油中的主要成分进行了分析。结果表明：应用响应面法优化酸枣仁中脂肪油的SFE-CO2最佳提取方案为提取温度40 ℃，萃取压力395 bar，动态萃取时间2.5 h，酸枣仁样品粉末粒度为40目，其中脂肪油提取效果较好，提取率约为20%;应用GC-MS检测结果表明，脂肪油中的主要成分为10-十八碳烯酸、十八烷酸、9,12-十八碳二烯酸、十六烷酸等;脂肪油是其主要成分，占相对含量的60%以上;SFE-CO2萃取技术具有高效、方便、提取效率高、绿色环保等显著优点，适合于酸枣仁中脂肪油的提取。
- Abstract:
- Taking Zizyphi spinosi Semen as material,supercritical CO2 fluid extraction of the fatty oil from Zizyphi spinosi Semen was carried out,the extraction temperature,pressure,time and the sample particle size were independent determined and the conditions were evaluated by response surface methodology(RSM).The results showed that the maximum extraction yield of about 20% was achieved at extract temperature of 40 ℃,pressure of 395 bar,dynamic extraction time of 2.5 h and power particle size of 40 mesh.The chemical compositions of fatty oil were investigated by GC-MS.The results indicated that supercritical CO2 fluid extraction method was the suitable method,the main fatty acids were 10-octadecenoic acid,octadecanoic acid,9,12-octadecadienoic acid and palmitic acid,respectively.Supercritical fluid extraction was proved to an effective technique for extracting fatty oil from Zizyphi spinosi Semen.
参考文献/References:
[1]宋立人.中华本草[M].上海:上海科学技术出版社,1999:262. [2]张照荣,周凤琴,李建秀，等.酸枣仁品种整理及质量研究[J].山东中医药大学学报，1997，21(5):388. [3]梁柱华，杨建设，杨艺虹，等.酸枣仁中总黄酮的提取率测定研究[J].分析测试技术与仪器，2008,14(1)：34-37. [4]李兰芳，赵淑兰，吴树勋，等.酸枣仁脂肪油化学成分的研究[J].中药材，1993，16(3):29-30． [5]SHARIF M A,JUNG I Y,HYO J K,et al.Antiinflammatory activity of seed essential oil from Zizyphus jujube[J]．Food Chem Toxicol，2010，48:639-643． [6]吴和珍，陈婧，杨艳芳，等.超临界二氧化碳萃取酸枣仁油的指纹图谱研究[J].中国医院药学杂志，2007，27(1):24-27． [7]侯冬岩，回瑞华，杨梅，等.酸枣仁中挥发性化学成分分析[J].分析试验室，2003，22(3):84-86． [8]陈振德，许重远，谢立.超临界流体CO2萃取酸枣仁脂肪油化学成分的研究[J].中草药，2001，32(11):976-977． [9]MEZZOMO N，MILEO B R，FRIEDRICH M T，et al．Supercritical fluid extraction of peach(Prunus persica)almond oil:Process yield and extract composition[J]．Journal of Bioresource Technology，2010，101：5622-5632． [10] SNCHEZ-VICENTE Y，CABANAS A，RENUNCIO J A R，et al．Supercritical fluid extraction of peach(Prunus persica)seed oil using carbon dioxide and ethanol[J]．The Journal of Supercritical Fluids，2009，49：167-173． [11]贡济宇,赵启铎,蔡广知，等.超临界萃取酸枣仁油及其成分研究[J].长春中医学院学报,2005(21):58-59. [12]钟广华,梁宝钻,葛发欢.酸枣仁油的超临界CO2萃取[J].中药材,2002(25):407-408.
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备注/Memo
第一作者简介：白鹤龙(1980-)，男，吉林长春人，硕士研究生，研究方向为天然药物分析。E-mail：baihelong2000@126.com.责任作者：时东方(1978-)，男，吉林长春人，博士，副教授，研究方向为天然药物分析。E-mail：shidongfang3@163.com.基金项目：吉林省教育厅资助项目（吉教科合字[2013]第254号）。