SHA Jin-long,LI Jian,LI Zhi-gang.Effect of Different Organic Materials Treatment Patterns on Bacterial Diversity in Sandy Soil[J].Northern Horticulture,2014,38(03):97-101.
不同方式有机物料处理对沙化土壤细菌多样性的影响
- Title:
- Effect of Different Organic Materials Treatment Patterns on Bacterial Diversity in Sandy Soil
- 文章编号:
- 1001-0009(2014)03-0097-05
- 关键词:
- 有机物料; 细菌多样性; 变性梯度凝胶电泳（DGGE）; 16S rDNA
- Keywords:
- organic materials; bacterial diversity; denatured gradient gel electrophoresis (DGGE); 16S rDNA
- 分类号:
- S 154.1
- 文献标志码:
- A
- 摘要:
- 以宁夏银川腹地的沙化土壤为试材，使用变性梯度凝胶电泳法(DGGE)，以不做任何处理的土壤为对照（CK），研究了添加杨树碎片、杨树碎片＋柳树树枝覆盖、树枝混合粉碎材料覆盖的3种有机物处理方式对沙化土壤细菌多样性的影响。结果表明：3个处理均不同程度改变了土壤细菌群落结构，群落结构的丰富度大小依次为树枝混合粉碎材料覆盖＞杨树碎片+柳树树枝覆盖＞CK＞添加杨树碎片；聚类分析结果显示，CK和树枝混合粉碎材料覆盖，添加杨树碎片和杨树碎片+柳树树枝覆盖分别被聚类到一起，相似性分别是63%和67%；并且分离鉴定出了与降解有机物料相关的菌（Kocuria sp.NEAU-ST5-33）相似性为96%的菌。
- Abstract:
- Taking the sandy soil in Yinchuan interior desert as material,the effect of sandy soil bacterial diversity in different organic materials treatments(1.with 5% poplar chips mixed with soil；2.with 5% poplar chips mixed with soil and covered with willow branches on surface；3.with soil surface covered by wood chips) were studied,with no treatment as control(CK),the soil bacterial in defferent treatment were studied by the denatured gradient gel electrophoresis (DGGE).The results showed that under the treatments 1，2，3，the structure of bacterial community changed in different degrees.The abundance of community structure was highest in treatment 3，then in 2 and lowest in 1.The results of clustering analysis showed that，two groups were clustered.They respectively were CK and 3，which similarity was 63% and treatment 1 and 2，which similarity was 67%.One bacteria which had the 96% similarity with bacteria related to organic materials decomposition was isolated and identified.
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备注/Memo
第一作者简介：沙金龙（1989-），男，宁夏西吉人，硕士研究生，现主要从事生物化学与分子生物学等研究工作。E-mail：shajinlong12@aliyun.com.