YU Shu-hui,ZUO Man-man,YAN Hui,et al.Over-expression PtAUX1 of Populus tomentosa Carr.Affects the Reaction of Light[J].Northern Horticulture,2012,36(13):122-124.
毛白杨PtAUX1超表达对光照反应的影响
- Title:
- Over-expression PtAUX1 of Populus tomentosa Carr.Affects the Reaction of Light
- 文章编号:
- 1001-0009（2012）13-0122-03
- Keywords:
- auxin; auxin polar transport; Populus tomentosa Carr.
- 分类号:
- S 792.117
- 文献标志码:
- A
- 摘要:
- 利用构建好的PtAUX1的表达载体，转化毛白杨，获得转基因植株。通过PCR技术检测PtAUX1的转基因植株，分析转化植株在不同的光照长度和光照强度下的形态变化。结果表明：PtAUX1基因影响毛白杨对光照的敏感性，在低光照强度下，转基因毛白杨生长缓慢且叶片卷曲；表明PtAUX1基因通过生长素输入载体的不对称分布影响生长素的极性运输，进而参与叶片极性的建立，影响了器官形态和发育过程。
- Abstract:
- PtAUX1 vector constructed by our laboratory were transformed into Poplar，then get transgenic plants.The transgenic plants were contected by PCR，and the phenotypes were analyzed in the different illumination length and illumination intensity.The results showed that the transgenic plants influenced the sensitivity of light，leaves were curling in the low light intensity.These results indicated that PtAUX1 influence auxin polar transport by nonuniform distribution of import carrier，then participated in leaf polarity establishment，and influenced morphology and development of the organs.
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备注/Memo
第一作者简介：于淑惠（1984-），女，在读硕士，现主要从事园林分子植物育种研究。E-mail：shuhui19841015@126.com.
责任作者：樊金会（1965-），男，博士，副教授，现主要从事园林分子植物育种研究工作。E-mail：hjf2003@sdau.edu.cn.
基金项目：山东省科技计划资助项目（J07YF08）。
收稿日期：2012-04-26