WANG Ke-feng,ZHAO Chun-li,CHEN Li-fei,et al.Study on Rapid Propagationin vitro of Mukdenia rossii（Oliv.）Koidz[J].Northern Horticulture,2012,36(05):135-136.
槭叶草离体快繁的研究
- Title:
- Study on Rapid Propagationin vitro of Mukdenia rossii（Oliv.）Koidz
- 文章编号:
- 1001-0009（2012）05-0135-02
- Keywords:
- Mukdenia rossii（Oliv.）Koidz; seedling; tissue culture
- 分类号:
- S 681.903.6
- 文献标志码:
- A
- 摘要:
- 以槭叶草实生无菌苗为外植体，MS培养基为基础，通过添加不同种类和浓度的激素，筛选出槭叶草组织培养的最适培养基。结果表明：种子较好的灭菌时间为0.1%升汞 6 min；增殖的最适培养基为MS+6-BA 0.1 g/L+NAA 0.1 g/L，其增殖倍数高达5.1，并且苗生长状态良好；生根最适培养基为1/2MS。
- Abstract:
- The experiment was based on MS culture media and Mukdenia rossii（Oliv.）Koidz.Used explants were from seedlings of the germination seeds in vitro.An appropriate culture medium was obtained via regulating the concentrations of cytokinin and auxin.The optimal sterilization method for seeds Mukdenia rossii（Oliv.）Koidz was 1% HgCl2 6 min.The propagation coefficient of 5.1 times indicated that MS+6-BA 0.1 mg/ L+NAA 0.1 mg/ L was the optimal culture medium for subculture proliferation.The best rooting medium was 1/2MS.The survival rate of the plantlets was shown to be up to 97%.
参考文献/References:
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[3]黄弘轩,任同庆,韩文志.槭叶草对兔心房肌的正性肌力作用[J].吉林医学院学报,1988,2(2):21-23.
[4]李爱民,李昌禹,路文鹏.槭叶草的繁殖[J].特种经济动植物,2008（7）:40.
[5]宫敬俐.槭叶草驯化栽培技术研究[D].北京:中国农业科学研究院,2007.
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备注/Memo
第一作者简介：王克凤（1985-），女，在读硕士，现主要从事植物组织培养研究工作。
责任作者：顾德峰（1956-），男，教授，现主要从事园林植物组织培养工作。E-mail:wkf-2009@163.com。
基金项目：吉林省科学技术厅资助项目（20100259）。
收稿日期：2011-12-08