TANG Yiheng,CHENG Dandan,XU Tao,et al.Identification of Pathogenic Fungi Causing Rot Disease in Dictyophora rubrovolvata and Study on Fungicide Sensitivity[J].Northern Horticulture,2026,(6):91-100.[doi:10.11937/bfyy.20253198]
红托竹荪腐烂病病原真菌鉴定及药剂敏感性研究
- Title:
- Identification of Pathogenic Fungi Causing Rot Disease in Dictyophora rubrovolvata and Study on Fungicide Sensitivity
- 文章编号:
- 1001-0009(2026)06-0091-10
- 分类号:
- S436.411
- 文献标志码:
- A
- 摘要:
- 以安徽省合肥市长丰县红托竹荪种植基地采集的腐烂红托竹荪菌蛋为试材，采用组织分离法进行病原菌的分离和纯化，通过形态特征观察及EF1-α序列比对，鉴定了红托竹荪腐烂病的病原;并采用菌丝生长速率法，研究了10种化学杀菌剂和8种植物源杀菌剂对该病原真菌的抑制率，以期为高效防控红托竹荪腐烂病提供参考依据。结果表明：红托竹荪腐烂病的病原真菌为钩状木霉Trichoderma hamatum；咪鲜胺、苯甲丙环唑、多菌灵和百菌清可高效防治该真菌病害。其中，45%咪鲜胺水乳剂表现最优，在8 000倍稀释浓度下仍保持80%以上抑制率。植物源药剂中，厚朴酚、香芹酚和蛇床子素表现出显著抑菌活性；厚朴酚在低浓度（10 μg·mL-1）时抑制率达60%，且随浓度升高效果稳定。该研究首次提出厚朴酚具有高效防控钩状木霉的潜力，其天然来源特性为绿色防控提供了新方向。
- Abstract:
- Taking the rotten Dictyophora rubrovolvata egg samples collected from the cultivation base in Changfeng County,Hefei City,Anhui Province as test material,the pathogenic bacteria were isolated and purified by tissue separation method.Through morphological characteristics observation and EF1-α sequence comparison,the rot disease of Dictyophora rubrovolvata was identified.The inhibitory rate of 10 chemical fungicides and 8 plant-based fungicides on this pathogenic fungus was studied by the mycelium growth rate method,in order to provide a reference for efficient control of the rot disease of Dictyophora rubrovolvata.The results showed that the pathogenic fungus of Dictyophora rubrovolvata was identified as Trichoderma hamatum.Prochloraz,Difenoconazole Propiconazole,Carbendazim and Chlorothalonil exhibited high efficacy against the pathogenic fungus.Among these,45% Prochloraz EW showed optimal performance,maintaining over 80% inhibition rate even at an 8 000-fold dilution concentration.For botanical agents,Magnolol,Carvacrol and Osthole displayed significant antifungal activity.Magnolol achieved a 60% inhibition rate at a low concentration (10 μg·mL-1),with stable efficacy observed across increasing concentrations.The study first proposed that Magnolol has the potential for high-efficiency control of Trichoderma hamatum,and its natural origin offered a novel direction for green disease control strategies.
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备注/Memo
第一作者简介：唐义恒(1996-)，男，本科，助理工程师，现主要从事食用菌高效生产及病虫害绿色防控等研究工作。E-mail：1941700041@qq.com.责任作者：陈名君（1980-），女，博士，副教授，现主要从事真菌分子系统学及病虫害绿色防控等研究工作。E-mail：mjchen@ahau.edu.cn.基金项目：安徽省高等学校自然科学研究重点资助项目（2024AH050480）；校企合作技术推广资助项目（KJ24272）；2025年国家级大学生创新创业资助项目（202510364066）。收稿日期：2025-09-08