YANG Sheng,CHEN Jiaru,LIANG Chunhui,et al.Screening of Genes Related to the Elongation Growth of Cucumbers Under Low-light Stress[J].Northern Horticulture,2025,(23):30-38.[doi:10.11937/bfyy.20251513]
弱光胁迫下黄瓜伸长生长相关基因筛选
- Title:
- Screening of Genes Related to the Elongation Growth of Cucumbers Under Low-light Stress
- 文章编号:
- 1001-0009(2025)23-0030-09
- Keywords:
- low-light stress; cucumber; elongation growth; RNA-Seq analysis; gene screening
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以高代纯合的耐弱光黄瓜品系M67与不耐弱光品系M14为试材，通过弱光胁迫分析黄瓜株高、节间长度及茎组织内激素含量的表型变化，结合转录组测序分析及qRT-PCR验证，探讨弱光对黄瓜伸长生长及相关基因表达的影响，以期为耐弱光分子标记开发及探索弱光抗性机制提供参考依据。结果表明：弱光胁迫显著抑制M14的伸长生长，其株高与第2节间长分别下降51.3%和37.3%，而M67的生长未受显著影响。M14茎组织中生长素（IAA）与赤霉素（GA）含量极显著降低，吲哚-3-丙酸（IPA）和玉米素核苷（ZR）含量显著降低，油菜素内酯（BR）含量则极显著升高；M67茎组织中GA含量上升，IPA和ZR含量分别显著和极显著降低，IAA和BR含量则无明显变化。通过转录组分析共鉴定出932个差异表达基因（DEGs），KEGG富集于苯丙氨酸代谢、淀粉和蔗糖代谢及植物激素信号传导等相关通路，结合表型分析共筛选出10个弱光相关候选基因，涉及生长素、赤霉素及细胞分裂素等激素信号通路相关基因。其中，弱光胁迫后生长素正调控因子SAUR表达量在不耐弱光品系M14茎组织中显著降低，负调控因子AUX/IAA和GH3显著升高；DELLA蛋白相关基因Csa3G043910/Csa5G623560在M14植株茎中显著下调表达，这些基因的表达可能是不耐弱光品系植株受弱光胁迫后植株显著矮化的主要原因。
- Abstract:
- Taking the high-generation homozygous low-light-tolerant cucumber strain M67 and the low-light-intolerant strain M14 as the test materials,through the phenotypic changes analysis of plant height，internode length and hormone content in stem tissue of cucumbers after low-light stress，combined with transcriptome sequencing analysis and qRT-PCR verification，the effects of low-light on the elongation growth of cucumbers and the expression of related genes were explored,in order to provide reference for the molecular markers development of low-light resistance and the exploration of the mechanism of low-light resistance.The results showed that low-light stress significantly inhibited the elongation growth of M14，with the plant height and the length of the second internode decreasing by 51.3% and 37.3% respectively，while the growth of M67 was not significantly affected.In the stem tissue of M14,the contents of auxin (IAA),gibberellin (GA) decreased extremely significantly，and the contents of indole-3-propionic acid (IPA) and zeatin riboside (ZR) decreased significantly,while the levels of brassinolide (BR) increased extremely significantly.In the stem tissue of M67,the GA content increased,the contents of IPA and ZR decreased significantly and extremely significantly respectively,there was no obvious change in IAA and BR.Transcriptome analysis identified a total of 932 differentially expressed genes (DEGs)，with KEGG enriched in pathways related to phenylalanine metabolism，starch and sucrose metabolism，and plant hormone signaling.Combined with phenotypic analysis，a total of 10 candidate genes related to low light were screened out，involving genes related to hormone signaling pathways such as auxin，gibberellin，and cytokinin.Among them，after low-light stress，the expression level of the positive auxin regulatory factor SAUR significantly decreased in the stem tissues of the M14 strain，while the negative regulatory factors AUX/IAA and GH3 significantly increased.The DELLA protein-related genes Csa3G043910/Csa5G623560 were significantly downregulated in the stem of M14 plants.The expression of these genes might be the main reason for the significant dwarfization of plants in low-light-tolerant varieties after being subjected to low-light stress.
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备注/Memo
第一作者简介：杨升（1971-），男，硕士，高级农艺师，现主要从事蔬菜栽培及育种等研究工作。E-mail:yangsheng1971@163.com.责任作者：李丹丹（1981-），女，博士，教授，现主要从事黄瓜遗传与分子育种等研究工作。E-mail:lidandan342@126.com.基金项目：国家自然科学基金青年基金资助项目（32002043）。收稿日期：2025-04-23