SHI Xiaoyan,SHI Jie,LI Qianjin,et al.Identification of Apple Water Rot Pathogen and Screening of Control Agents[J].Northern Horticulture,2025,(2):67-75.[doi:10.11937/bfyy.20242686]
苹果水烂病病原菌鉴定及防治药剂筛选
- Title:
- Identification of Apple Water Rot Pathogen and Screening of Control Agents
- 文章编号:
- 1001-0009(2025)02-0067-09
- Keywords:
- ‘Fuji’ apples; water rot disease; biological characteristics; toxicity measurement; pathogen isolation and identification
- 分类号:
- S 661.1
- 文献标志码:
- A
- 摘要:
- 以陕西省洛川县部分果业公司贮藏冷库中发生水烂病的苹果果实为试材，采用组织分离法进行病原菌鉴定，并开展病原菌生物学特性、品种抗性和药剂筛选等方面的研究工作，明确陕西省洛川县‘富士’苹果水烂病病原菌的种类，筛选出防治水烂病的有效杀菌剂，以期为该病的诊断和防控提供参考依据。结果表明：引起‘富士’苹果贮藏期水烂病的病原菌属于担子菌门、伞菌纲、伞菌目、挂钟菌科；该病原菌的最适生长温度为20~25 ℃，最适pH为6~7；室内毒力测定结果显示不同杀菌剂的抑菌效果存在差异，效果最好的是四霉素及多抗霉素，EC50值分别为0.033 3 μg·mL-1和0.037 7 μg·mL-1；纳他霉素的活性次之，其EC50值为0.832 5 μg·mL-1；曲酸、壳聚糖效果较差。
- Abstract:
- The apple fruits with water rot in the cold storage of some fruit companies in Luochuan county，Shaanxi Province were used as the test materials.The pathogens were identified by tissue isolation method，and the biological characteristics，variety resistance and fungicide screening of pathogens were carried out to clarify the species of pathogens of ‘Fuji’ apple water rot in Luochuan county，Shaanxi Province，and screen out effective fungicides for the prevention and control of water rot，in order to provide reference for the diagnosis and prevention and control of the disease.The results showed that the pathogens causing water rot of the ‘Fuji’ apple during storage belonged to Basidiomycota，Agaricomycetes，Agaricomycetes，and Campylobacteraceae.The optimum growth temperature of the pathogen was 20-25 ℃ and the optimum pH was 6-7.The results of toxicity measurement showed that the antibacterial effects of different fungicides were different.The best effect was tetramycin and polyoxin，with EC50 value of 0.033 3 μg·mL-1 and 0.037 7 μg·mL-1，respectively.The EC50 value of natamycin was 0.832 5 μg ·mL-1.The effect of kojic acid and chitosan was poor.
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备注/Memo
第一作者简介：石晓燕（1998-），女，硕士研究生，研究方向为园艺产品采后生理及贮藏保鲜技术。Email:1846156526@qq.com.责任作者：任小林（1964-），男，博士，教授，博士生导师，现主要从事园艺产品采后生理、贮藏保鲜及果实成熟衰老分子生物学等研究工作。Email:tjw689@126.com.基金项目：陕西省关键核心技术攻关资助项目（2024NYGG003）；陕西省苹果产业转型升级关键技术研发及产业化示范科技重大专项资助项目（2020zdzx030501）；财政部和农业农村部国家苹果产业技术体系资助项目（CARS27）。收稿日期：2024-06-26