[1]李忆,尹全,张瑶,等.黄肉猕猴桃‘金实4号’果实采后蒂腐病病原菌的分离及鉴定[J].北方园艺,2024,(13):91-96.[doi:10.11937/bfyy.20240666]
　LI Yi,YIN Quan,ZHANG Yao,et al.Isolation and Identification of Pathogenic Fungi Causing Post-harvest Stem-end Rot of ‘Jinshi No.4’ Kiwifruit[J].Northern Horticulture,2024,(13):91-96.[doi:10.11937/bfyy.20240666]
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黄肉猕猴桃‘金实4号’果实采后蒂腐病病原菌的分离及鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年13 页码: 91-96 栏目: 出版日期: 2024-07-15
Title:
Isolation and Identification of Pathogenic Fungi Causing Post-harvest Stem-end Rot of ‘Jinshi No.4’ Kiwifruit
文章编号:
1001-0009(2024)13-0091-06
作者:
李忆1; 尹全2; 张瑶3; 孙雷明4; 庄启国3
（1.四川省农业科学院 植物保护研究所，四川 成都 610066；2.四川省农业科学院 质量标准与检测技术研究所，四川 成都 610066；3.四川省自然资源科学研究院 中国-新西兰猕猴桃“一带一路”联合实验室，猕猴桃育种及利用四川省重点实验室，四川 成都 610015；4.中国农业科学院 郑州果树研究所，河南 郑州 450009）
Author(s):
LI Yi1; YIN Quan2; ZHANG Yao3; SUN Leiming4; ZHUANG Qiguo3
610066；2.Institute of Quality Standard and Testing Technology Research，Sichuan Academy of Agricultural Sciences，Chengdu，Sichuan 610066；3.China-New Zealand Belt and Road Joint Laboratory on Kiwifruit/Kiwifruit Breeding and Utilization Key Laboratory of Sichuan Province,Sichuan Province Academy of Natural Resource Science，Chengdu，Sichuan 610015；4.Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou,Henan 450009)
关键词:
‘金实4号’猕猴桃; 腐烂病; 病原菌; 鉴定
Keywords:
‘Jinshi No.4’ kiwifruit; rot disease; pathogenic fungi; identification
分类号:
S 663.4
DOI:
10.11937/bfyy.20240666
文献标志码:
A
摘要:
以猕猴桃新品种‘金实4号’采后病果为试材，取果实果蒂病组织分离腐烂病病原菌，按照柯赫氏法则确定分离病株的致病性，同时采用形态学结合分子生物学鉴定技术对病原菌种类进行鉴定,明确黄肉猕猴桃果实采后贮藏期引起腐烂的病菌种类，以期为黄肉猕猴桃采后腐烂病防治研究提供参考依据。结果表明：共有8种软腐病病原菌被鉴定，分别是拟茎点霉菌（Phomopsis sp.）、间座壳菌（Diaporthe cotoneastri）、小孢拟盘多毛孢（Pestalotiopsis microspora）、灰葡萄孢（Botrytis cinerea）、新壳梭孢（Neofusicoccum parvum）、链格孢（Alternaria sp.）、镰刀菌（Fusarium sp.）、葡萄座腔菌（Botryosphaeria dothidea）。
Abstract:
Naturally diseased fruit of ‘Jinshi No.4’ kiwifruit were used as test material.The fungi were isolated from a large number of rotten samples，and were identified through their morphologic characteristics and molecular analysis.Koch′s postulate was performed to confirm the identification.The fungi which cause post-harvest stem-end rot of yellow-flesh kiwifruit was identified,in order to provide reference for the research on the prevention and control of post-harvest rot disease of yellow-flesh kiwifruit．The results showed that eight different fungi were causable for stem-end rot of kiwifruit.They were Phomopsis sp.,Diaporthe cotoneastri,Pestalotiopsis microspora,Botrytis cinerea,Neofusicoccum parvum，Alternaria sp.,Fusarium sp.and Botryosphaeria dothidea.

参考文献/References:

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备注/Memo

第一作者简介：李忆（1982-），女，硕士，副教授，现主要从事植物病虫害防治等研究工作。E-mail：44182557@qq.com.责任作者：庄启国（1979-），男，博士，研究员，现主要从事抗性育种及病虫害防控等研究工作。E-mail:qgzhuang@126.com.基金项目：国家重点研发计划资助项目（2022YFD1600701）；四川省科技计划资助项目（2023JDZH0012，2024ZHYS0012，2024YFHZ0227）。收稿日期：2024-02-18

更新日期/Last Update: 2024-07-18