XING Lingkai,CAO Shun,LI Weiwen,et al.Microbial Diversity Analysis of Trichosanthes kirilowii Maxim. Fruits and Identification of Pathogenic Pathomycete[J].Northern Horticulture,2024,(18):100-107.[doi:10.11937/bfyy.20240552]
栝楼病果微生物多样性分析及病原菌的分离鉴定
- Title:
- Microbial Diversity Analysis of Trichosanthes kirilowii Maxim. Fruits and Identification of Pathogenic Pathomycete
- 文章编号:
- 1001-0009(2024)18-0100-08
- Keywords:
- Trichosanthes kirilowii Maxim.; pathomycete; next generation sequencing; molecular identification
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 以栝楼健康果和病果为试材，采用高通量测序技术与分子鉴定的方法，研究栝楼病果与健康果表面微生物多样性差异，探究引起栝楼果实致病菌的种类,以期为栝楼病害的科学防治提供参考依据。结果表明:从所有样本中共得到8个门、24个纲、54个目、133个科、206个属、286个种，其中属水平排列前5的依次为间座壳菌属（Diaporthe）、枝孢菌属（Cladosporium）、蔓枯菌属（Stagonosporopsis）、炭疽菌属（Colletotrichum）、酵母菌属（Hannaella）。患病果实表面微生物的集中度高，与健康组微生物群落结构具有明显差异，其〖JP+2〗中丰度较高微生物主要为间座壳属（Diaporthe）、炭疽菌属（Colletotrichum）真菌；选择4株分离纯化后的典型菌株进行DNA分子鉴定，结果显示为炭疽属（Colletotrichum）、间座壳属（Diaporthe）真菌，验证了微生物多样性分析结果。综合分析可知，引起潜山市栝楼果实病害的病原菌为间座壳属（Diaporthe）、炭疽菌属（Colletotrichum）真菌。
- Abstract:
- Taking healthy and diseased fruits of Trichosanthes kirilowii as the test material,next generation sequencing (NGS) technology and molecular identification method were used to analyze the healthy and diseased fruits to explore the species of pathomycete of T.kirilowii fruits,in order to provide reference for scientific prevention and control of diseases in Trichosanthes kirilowii.The results showed that a total of 8 phyla,24 orders,54 classes,133 families,206 genera,and 286 species were obtained from all samples.Among them,the top five genera were Diaporthe,Cladosporium,Stagonosporopsis,Colletotrichum and Hannaella.There was a high concentration of microorganisms on the diseased fruits,and the microbial community structure was significantly different from the healthy group.The microorganisms with high abundance were mainly Diaporthe and Colletotrichum.The fungus of Trichosanthes kirilowii Maxim. were isolated and purified.Four typical strains were selected for DNA molecular identification.The results showed that the fungi were Colletotrichum,Diaporthe,which verified the results of microbial diversity analysis.In summary,Diaporthe and Colletotrichum fungi were identified as the pathomycete causing the disease of T.kirilowii in Qianshan city.
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备注/Memo
第一作者简介：邢铃凯（1998-），男，硕士研究生，研究方向为中药资源开发与利用。E-mail：2453049529@qq.com.责任作者：谢冬梅（1975-），女，博士，副教授，现主要从事中药资源开发与中药质量评价等研究工作。E-mail:xiedongmei96@126.com.基金项目：安徽中医药大学-潜山市医药健康发展技术研究中心科研攻关资助项目(2021QSHZ01-2)。收稿日期：2024-02-01