WANG Jie,LIANG Liting,XIE Xinyi,et al.Isolation and Identification of a Pathogen of Cinnamon Anthracnose and Screening of Biocontrol Bacteria[J].Northern Horticulture,2024,(8):102-108.[doi:10.11937/bfyy.20233694]
一株肉桂炭疽病病原菌分离鉴定及生防菌筛选
- Title:
- Isolation and Identification of a Pathogen of Cinnamon Anthracnose and Screening of Biocontrol Bacteria
- 文章编号:
- 1001-0009(2024)08-0102-07
- 分类号:
- S 436.8
- 文献标志码:
- A
- 摘要:
- 以广西防城港人工栽培的肉桂病叶为试材,采用内生真菌分离纯化法、形态学观察、ITS测序和平板对峙等方法,研究了肉桂炭疽病病原菌的分类地位及其拮抗菌株的筛选,以期为肉桂炭疽病生防菌开发提供参考依据。结果表明:从广西防城港肉桂病叶中分离出6株真菌(B1~B6),B6明显致病。通过形态和分子生物学鉴定该菌株为炭疽菌Colletotrichum gloeosporioides。平板对峙结果表明,RG256拮抗效果最佳,抑制率为77.05%±5.06%,鉴定为Gliocladiopsis sp.。综上所述,明确广西防城港肉桂炭疽病的病原真菌为炭疽病菌 (Colletotrichum gloeosporioides),并筛选到对肉桂炭疽病病原真菌有拮抗作用的生防菌株,为肉桂炭疽病的绿色防治提供参考依据。
- Abstract:
- Guangxi Fangchenggang diseased cinnamon leaves in cultivation were used as experimental materials.The taxonomic status of cinnamon anthracnose pathogen and the screening of its antagonistic strains were investigated by using the endophytic fungi isolation and purification technology method,morphological observation,ITS sequencing and plate confrontation,in order to provide reference for the development of biocontrol fungi against cinnamon anthracnose.The results showed that 6 strains (B1-B6) of fungi were isolated from cinnamon diseased leaves in Fangchenggang,Guangxi,and B6 was clearly pathogenic.The strain was identified as Colletotrichum gloeosporioides by morphology and molecular biology.The results of the tablet confrontation showed that RG256 had the best antagonistic effect,with an inhibition rate of 77.05%±5.06%,and was identified as Gliocladiopsis sp..In summary,it had been clarified that the pathogenic fungus of cinnamon anthracnose in Fangchenggang,Guangxi was Colletotrichum gloeosporioides,and biocontrol strains with antagonistic effects against the pathogenic fungi of cinnamon anthracnose had been screened,providing reference for the green control of cinnamon anthracnose.
参考文献/References:
[1]国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2020.[2]高铭哲,李婷,田晨琪,等.肉桂化学成分与药理作用研究进展[J].亚太传统医药,2021,17(11):201-205.[3]梁晓静,安家成,黎贵卿,等.肉桂特色资源加工利用产业发展现状[J].生物质化学工程,2020,54(6):18-24.[4]单体江,叶聪,冯冲,等.肉桂、樟树和阴香病害及其综合防治研究进展[J].江苏农业科学,2021,49(15):40-47.[5]BIAN J Y,FANG Y L,SONG Q,et al.The fungal endophyte Epicoccum dendrobii as a potential biocontrol agent against Colletotrichum gloeosporioides[J].Phytopathology,2021,111(2):293-303.[6]胡丽杰,闫思远,李嘉泓,等.枸杞内生真菌对胶孢炭疽菌Colletotrichum gloeosporioides的拮抗作用及生防潜力[J].植物保护,2020,46(1):125-133.[7]吕佳,罗碧,钱家萍,等.广东肉桂内生真菌多样性及其抗肉桂枝枯病菌初步研究[J].菌物学报,2022,41(3):435-449.[8]冯亚萍.伊犁黑果小檗内生真菌的分离鉴定、化学成分及活性研究[D].伊犁:伊犁师范大学,2022.[9]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.[10]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001.[11]苏秀丽,李惠敏,唐辉,等.黄花倒水莲内生真菌多样性及抑菌活性分析[J].北方园艺,2022(6):99-106.[12]施祖荣,张云霞,黄江华,等.广东地区墨兰和大花蕙兰炭疽病菌的鉴定[J].仲恺农业工程学院学报,2013,26(2):22-25.[13]杨卓颖,梁晓静,曾祥艳,等.肉桂一年生实生苗年生长节律及其生物量分配规律[J].中国农学通报,2023,39(8):21-26.[14]PERFECT S E,HUGHES H B,O’CONNELL R J,et al.Colletotrichum: A model genus for studies on pathology and fungal-plant interactions[J].Fungal Genetics and Biology,1999,27(2/3):186-198.[15]裴张新,王志华,于静亚,等.园林植物炭疽病研究进展[J].中国森林病虫,2023,42(6):33-38.[16]MUNKVOLD G P.Fusarium species and their associated mycotoxins[M]//Mycotoxigenic Fungi.New York:Humana Press,2017:51-106.[17]GAO Y,ZHAI F Y,ZHANG Y B,et al.Neopestalotiopsis rosae causing black spot on leaf and fruit of pecan (Carya illinoinensis) in China[J].Plant Disease,2022:PDIS07211541PDN.[18]ZHANG D,HE J,CHENG P,et al.4-methylumbelliferone (4-MU) enhances drought tolerance of apple by regulating rhizosphere microbial diversity and root architecture[J].Horticulture Research,2023,10(7):uhad099.[19]WANG Q,YANG R,PENG W,et al.Tea plants with gray blight have altered root exudates that recruit a beneficial rhizosphere microbiome to prime immunity against aboveground pathogen infection[J].Frontiers in Microbiology,2021,12:774438.[20]LIU L,CHEN Z,LIU W,et al.Cephalosporin C biosynthesis and fermentation in Acremonium chrysogenum[J].Applied Microbiology and Biotechnology,2022,106(19):6413-6426.[21]KIM H J,LI X J,KIM D C,et al.PTP1B inhibitory secondary metabolites from an Antarctic fungal strain Acremonium sp.SF-7394[J].Molecules,2021,26(18):5505.
相似文献/References:
[1]梁晓静,韦晓娟,陈金艳,等.不同施肥处理对肉桂容器苗早期生长的影响[J].北方园艺,2014,38(02):165.
LIANG Xiao-jing,WEI Xiao-juan,CHEN Jin-yan,et al.Effect of Different Fertilizer Treatments on the Seedling Growth of Cinnamomum cassia Presl[J].Northern Horticulture,2014,38(8):165.
[2]周慧杰,郭 玲.苹果炭疽病菌生物学特性研究[J].北方园艺,2012,36(22):133.
ZHOU Hui-jie,GUO Ling.Study on Biological Characteristics of Apple Anthrax Bacteria[J].Northern Horticulture,2012,36(8):133.
[3]田 玉 红,李 日 南,邹 克 兴,等.蒸馏时间对肉桂油化学成分及加香效果的影响[J].北方园艺,2011,35(05):0.[doi:10.11937/bfyy.201105042]
TIAN Yu -hong,LI Ri -nan,ZOU Ke -xing,et al.Influence of Distillation Time on the Composition of Essential Oil from Cinnamomumcassia Presl and Its T obacco Flavoring Effect[J].Northern Horticulture,2011,35(8):0.[doi:10.11937/bfyy.201105042]
[4]张宝清,翟雅鑫,薛丽芳,等.四季秋海棠炭疽病菌生物学特性及生防因子的筛选[J].北方园艺,2021,(10):66.[doi:10.11937/bfyy.20202508]
ZHANG Baoqing,ZHAI Yaxin,XUE Lifang,et al.Biological Characteristic of Colletotrichum truncatum on Begonia semperflorens and Screening of Biocontrol Factors[J].Northern Horticulture,2021,(8):66.[doi:10.11937/bfyy.20202508]
备注/Memo
第一作者简介:王洁(2000-),女,硕士研究生,研究方向为珍稀药用植物和药用真菌资源。E-mail:873167025@qq.com.责任作者:谭小明(1979-),男,博士,研究员,硕士生导师,现主要从事珍稀药用植物和药用真菌资源等研究工作。E-mail:txm1978@126.com.基金项目:广西科技重大专项资助项目(桂科AA22096029);中央引导地方科技发展资金资助项目(桂科ZY22096003);广西壮瑶药重点实验室资助项目(桂科基字[2014]32号)。收稿日期:2023-10-27