ZHANG Qin,KANG Mingli,ZHAO Ru,et al.Isolation and Identification of Pathogenic Bacteria Causing Postharvest Rot Disease in Green Walnuts[J].Northern Horticulture,2024,(4):74-80.[doi:10.11937/bfyy.20233560]
青皮核桃采后腐烂病病原菌分离与鉴定
- Title:
- Isolation and Identification of Pathogenic Bacteria Causing Postharvest Rot Disease in Green Walnuts
- 文章编号:
- 1001-0009(2024)04-0074-07
- 分类号:
- S 664.1
- 文献标志码:
- A
- 摘要:
- 以青皮核桃为试材，采用组织分离法分离病原菌，并依据柯赫式法则确定其致病性，结合病原菌形态学特征鉴定及rDNA-ITS序列测定分析鉴定病原菌的种类，以期为青皮核桃采后腐烂病的防控措施提供参考依据。结果表明：青皮核桃采后病害为炭疽病和黑斑病，分离出5种优势致病菌，根据病原菌形态学及rDNA-ITS序列分析法证实其中HT73、HT55为暹罗炭疽菌（Colletotrichum siamense）、HT12为胶孢炭疽（Colletotrichum gloeosporioides）、HL35为镰刀菌（Fusarium sp.）、HP92为葡萄座腔菌（Botryosphaeria dothidea）。该研究明确了引起青皮核桃腐烂病的主要致病菌，可为有效防止青皮核桃采后腐烂病提供参考依据。
- Abstract:
- Taking green walnut as the test material,pathogenic bacteria were isolated using tissue isolation method,and their pathogenicity was determined based on Koch′s rule.The morphological characteristics of the pathogenic bacteria and rDNA-ITS sequence analysis were combined to identify the types of pathogenic bacteria,in order to provide reference for the prevention and control measures of post harvest rot disease in green walnut.The results showed that the post harvest diseases of green walnut were anthracnose and black spot,and five dominant pathogenic bacteria were isolated.Based on the morphology of the pathogens and rDNA-ITS sequence analysis,it was confirmed that HT73 and HT55 were Colletotrichum siamense,HT12 was Colletotrichum gloeosporioides,HL35 was Fusarium sp,and HP92 was Botryospaeria dothidea.This study identified the main pathogenic bacteria causing green walnut rot disease,which could provide a basis for effectively preventing postharvest rot disease in green walnuts.
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备注/Memo
第一作者简介：张琴(1998-)，女，硕士研究生，研究方向为农产品采后保鲜与贮藏。E-mail:2466059837@qq.com.责任作者：康明丽（1973-），女，博士，教授，现主要从事农产品贮藏与加工等研究工作。E-mail kmingl@hebust.edu.cn.基金项目：河北省重点研发计划资助项目（21327101D）。收稿日期：2023-10-18