LI Bo,GAO Xiaomin,WANG Nan,et al.Community Composition and Diversity Analysis of Endophytes in Hippophae rhamnoides Seeds From Different Locations[J].Northern Horticulture,2024,(8):94-101.[doi:10.11937/bfyy.20233384]
不同产地沙棘种子内生菌群落组成与多样性分析
- Title:
- Community Composition and Diversity Analysis of Endophytes in Hippophae rhamnoides Seeds From Different Locations
- 文章编号:
- 1001-0009(2024)08-0094-08
- Keywords:
- Hippophae rhamnoides; seeds; endophytes; high-throughput sequencing; diversity
- 分类号:
- S 793.6
- 文献标志码:
- A
- 摘要:
- 以新疆、内蒙古、山西、陕西、甘肃6个产地的沙棘种子为试材，采用高通量测序及生物信息学分析方法，基于16S rRNA和ITS2序列，研究了沙棘种子内生细菌和真菌的菌群结构与多样性，以期为后续研究沙棘种子内生菌的生物活性，探寻沙棘内生功能菌群提供参考依据。结果表明：沙棘种子内生菌组成较为单一，内生细菌主要为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)等，内生真菌为子囊菌门(Ascomycota)、被孢霉门(Mortierellomycota)和担子菌门(Basidiomycota)；Alpha物种多样性分析结果表明，陕西志丹与新疆阿勒泰样品内生细菌的Shannon指数与Simpson指数存在显著差异；山西吕梁与陕西志丹样品内生真菌的Shannon指数和Chao1指数达到显著差异；NMDS分析显示不同样品的内生菌组成存在一定相似性；KEGG分析内生细菌共获得6个一级代谢系统和26个二级代谢通路。综上，不同产地沙棘种子内生菌多样性与群落组成存在差异性，这可能与其栖息地的环境因子密切相关。
- Abstract:
- In order to provide reference for the subsequent study of the biological activity of endophytes and explore the endophytic functional microbiota in Hippophae rhamnoides seeds，six samples were collected from Xinjiang，Inner Mongolia，Shanxi，Shaanxi and Gansu，the structure and diversity of endophytic bacteria and fungi were studied by high-throughput sequencing and bioinformatics analysis methods based on 16S rRNA and ITS2 sequence.The results showed that the composition of endophytes in H.rhamnoides seeds was relatively simple.The endophytic bacteria were mainly Proteobacteria，Firmicutes，Actinobacteria；the endophytic fungi were Ascomycete，Mortierellomycota and Basidiomycota.Alpha diversity analysis showed that the Shannon and Simpson indices of endophytic bacteria in Zhidan，Shaanxi and Altay，Xinjiang samples were significantly different.Meanwhile，the Shannon and Chao1 indices of endophytic fungi were significantly different between seed samples from Lyuliang，Shanxi and Zhidan，Shaanxi.Non-metric multidimensional scaling analysis showed certain similarity on the composition of endophytes in different seed samples.Kyoto Encyclopedia of Genes and Genomes analysis generated six primary metabolic systems and 26 secondary metabolic pathways in the endophytic bacteria.Accordingly，the diversity and community composition of endophytes in H.rhamnoides seeds from different regions were different，which may be closely related to their growing environments.
参考文献/References:
［1］中国科学院中国植物志编辑委员会.中国植物志-第七十三卷,第二分册［M］.北京:科学出版社,1983.［2］国家药典委员会.中华人民共和国药典·一部:2020年版［M］.北京:中国医药科技出版社,2020.［3］李晓燕,王林和,李连国,等.沙棘根系形态解剖特征与其生态适应性研究［J］.内蒙古农业大学学报(自然科学版),2007,28(4):62-66.［4］高佩,郭佳慧,何永超,等.中国沙棘根际四株假单胞菌的分离、鉴定及促生能力的比较［J］.北方园艺,2023(1):69-75.［5］王岚,张小民,林美珍,等.先锋植物沙棘Ⅰ:共生体是实现先锋作用的根本因素之一［J］.国际沙棘研究与开发,2006,4(1):10-17,24.［6］胡建忠.沙棘功能性食品开发探讨［J］.国际沙棘研究与开发,2007,5(4):16-20.［7］韩立柱,高欢,胡坤霞,等.沙棘籽油微乳的处方筛选及制备工艺研究［J］.中国现代中药,2020,22(12):2072-2079.［8］WANI Z A,ASHRAF N,MOHIUDDIN T,et al.Plant-endophyte symbiosis,an ecological perspective［J］.Applied Microbiology and Biotechnology,2015,99(7):2955-2965.［9］黄宝康,秦路平.药用植物内生菌的生物多样性及活性成分［J］.药学实践杂志,2006,24(4):193-196.［10］陈柯璇,汤雯婷,李丽娜,等.种子内生菌增强宿主植物重金属抗性的功能机制研究进展［J］.微生物学通报,2021,48(6):2187-2194.［11］陈鑫,李曦冉,杨姣姣.沙棘种子内生细菌对几种植物种子萌发的影响［J］.中国野生植物资源,2022,41(2):25-29.［12］杨镇,曹君.植物内生菌及其次级代谢产物的研究进展［J］.微生物学杂志,2016,36(4):1-6.［13］巩文峰,孙玉,王瑞琪,等.西藏油菜种子内生菌分离及具有多种促生特性的拮抗菌筛选［J］.植物保护学报,2022,49(4):1053-1062.［14］江曙,钱大玮,段金廒,等.植物内生菌与道地药材的相关性研究［J］.中草药,2008,39(8):1268-1272.［15］陈柯璇,李海燕.植物种子内生菌及其应用前景的研究进展［J］.中国野生植物资源,2021,40(11):40-44.［16］王红阳,康传志,王升,等.基于高通量测序和传统培养分离方法的药用植物内生菌资源研究策略［J］.中国中药杂志,2021,46(8):1910-1919.［17］龙锡,严希,洪佳丽,等.植物种子内生菌的研究进展［J］.浙江农业科学,2016,57(8):1319-1324.［18］THIJS S,de BEECK M O,BECKERS B,et al.Comparative evaluation of four bacteria-specific primer pairs for 16S rRNA gene surveys［J］.Frontiers in Microbiology,2017,8:494.［19］WANG F,MEN X,ZHANG G,et al.Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos［J］.AMB Express,2018,8(1):182.［20］TOJU H,TANABE A S,YAMAMOTO S,et al.High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples［J］.PLoS One,2012,7(7):e40863.［21］崔晋龙,郭顺星,肖培根.内生菌与植物的互作关系及对药用植物的影响［J］.药学学报,2017,52(2):214-221.［22］罗书岚,刘丽,李肖莉,等.不同时期白花前胡内生菌的多样性和差异性［J］.中国野生植物资源,2022,41(10):25-29.［23］陈绍通,戴军,姜雪萍,等.不同生长年限霍山石斛内生菌的多样性与差异性［J］.中国中药杂志,2019,44(6):1145-1150.［24］许国琪,刘怡萱,曹鹏熙,等.基于Illumina MiSeq测序技术对冰川棘豆(Oxytropis glacialis)不同组织内生菌多样性的研究［J］.生态学报,2021,41(12):4993-5003.［25］李琦,孙广宇.沙棘内生菌的分离与初步鉴定［J］.中国农学通报,2006,22(10):300-302.［26］张爱梅，韩雪英，张世虎，等.甘南高寒地区不同海拔西藏沙棘根瘤内生菌多样性［J］.生物资源，2018，40(2)：114-119.［27］张爱梅,郭保民,韩雪英,等.两种不同生境中国沙棘种子内生细菌的多样性［J］.生态学报,2020,40(15):5247-5257.［28］赵霞.水稻种子内生细菌多样性分析及核心微生物组的界定［D］.北京:中国农业科学院,2019.［29］冯玮娜,彭培好.四川牡丹根际微生物及种子内生菌组成［J］.东北林业大学学报,2020,48(1):88-94.［30］杜衎,耿燕楠,刘丽,等.北柴胡种子内生菌群落结构与多样性［J］.生物资源,2022,44(1):36-44.［31］涂然,黄文峰,冯正平,等.乌头内生细菌组成分析［J］.中国现代中药,2022,24(5):784-791.［32］徐祥林,骆海玉,颜小捷,等.罗汉果内生真菌多样性研究［J］.广西植物,2020,40(5):618-627.［33］刘蔚廷，陈家杰，冯佳威，等.云南、浙江、内蒙古禾本科植物内生真菌多样性研究［J］.菌物学报，2021，40(3)：502-513.［34］范雨,唐小乐,刘庆华,等.木本植物种子内生菌研究进展［J］.应用与环境生物学报,2022,28(5):1375-1383.
相似文献/References:
[1]田英翠,曹受金,曹基武.六种榉树种子形态特征及萌发特性的研究[J].北方园艺,2014,38(11):71.
TIAN Ying-cui,CAO Shou-jin,CAO Ji-wu.Study on Morphology and Germination Characteristics of Seeds of Six Species of Zelkova schneideriana [J].Northern Horticulture,2014,38(8):71.
[2]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(8):86.
[3]曾月娥,秦富仓,岳永杰.准格尔旗砒砂岩区沙棘林下草本植物组成及多样性分析[J].北方园艺,2013,37(22):98.
ZENG Yue-e,QIN Fu-cang,YUE Yong-jie.Analysis on Plant Composition and Diversity of Hippophae Forest in Sandstone Areas of Jungar Banner[J].Northern Horticulture,2013,37(8):98.
[4]马子林.藏药马尿泡种子吸水特性及抗旱性的初步研究[J].北方园艺,2013,37(22):164.
MA Zi-lin.Preliminary Study on Water Absorbing Character and Drought Resistance of the Seed of Tibetan Medicine Przewalskia tangutica Maxim[J].Northern Horticulture,2013,37(8):164.
[5]徐小玉,张凤银,张方俊.氯化钠胁迫对雏菊种子萌发及幼苗生长的影响[J].北方园艺,2014,38(14):52.
XU Xiao-yu,ZHANG Feng-yin,ZHANG Fang-jun.Effect of NaCl Stress on Seed Germination and Seedling Growth of Bellis perennis[J].Northern Horticulture,2014,38(8):52.
[6]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(8):1.
[7]张 芹,徐学山,李保会,等.不同处理条件对暴马丁香种子萌发的影响[J].北方园艺,2014,38(01):54.
ZHANG Qin,XU Xue-shan,LI Bao-hui,et al.Effects of Different Treatments Conditions on the Germination of Syringa reticulatassp. amurensisSeeds[J].Northern Horticulture,2014,38(8):54.
[8]崔霞,梁燕,王玲慧.不同浓度化学诱变剂甲基磺酸乙酯对番茄种子发芽的影响[J].北方园艺,2013,37(14):28.
CUI Xia,LIANG Yan,WANG Ling-hui.Effect of Different Concentrations of Chemical Mutagen EMS on Tomato Seed Germination[J].Northern Horticulture,2013,37(8):28.
[9]仲莎,姜守阳,刘桂花,等.化学药剂处理对黑籽南瓜种子发芽率的影响[J].北方园艺,2013,37(14):34.
ZHONG Sha,JIANG Shou-yang,LIU Gui-hua,et al.Effect of Chemicals on the Germination Rate of Cucurbita ficifolia Bauche Seeds[J].Northern Horticulture,2013,37(8):34.
[10]张瑜,卞勇,关力,等.药用植物高山红景天无土育苗研究[J].北方园艺,2013,37(10):150.
ZHANG Yu,BIAN Yong,GUAN Li,et al.Study on Soilless Seedling Rearing of Medicinal Plant Rhodiola sachaliensis[J].Northern Horticulture,2013,37(8):150.
备注/Memo
第一作者简介：李铂(1986-)，男，博士，副教授，现主要从事药用植物生理等研究工作。E-mail：boli_sntcm@163.com.责任作者：宋忠兴(1974-)，男，本科，副主任药师，现主要从事中药材质量评价等研究工作。E-mail：szx74816@sina.com.基金项目：内蒙古自治区2019年度重大科技专项资助项目(2019ZD005)；国家中药材产业技术体系建设专项资助项目(CARS-21)；陕西省高校青年科技创新团队资助项目(陕教［2019］90号)。收稿日期：2023-10-07