HU Lei,CHEN Xi,LIU Zhen,et al.Cloning and Functional Analysis of [STBX]MIR156c[STBZ] Gene in Chrysanthemum morifolium[J].Northern Horticulture,2023,(20):53-60.[doi:10.11937/bfyy.20231731]
菊花MIR156c基因的克隆与功能分析
- Title:
- Cloning and Functional Analysis of [STBX]MIR156c[STBZ] Gene in Chrysanthemum morifolium
- 文章编号:
- 1001-0009(2023)20-0053-08
- Keywords:
- Chrysanthemum; MIR156c; gene cloning; functional analysis
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以菊花‘Jinba’和拟南芥为试材，采用生物信息学、分子生物学方法，研究了菊花MIR156c基因的前体序列及二级结构、进化关系、表达模式、生长发育的功能，以期为菊花生长发育的分子机制提供参考依据。结果表明:克隆的菊花MIR156c的序列长度为212 bp。cmo-MIR156c能形成典型的茎环结构，在成熟序列的第11位具有特异性，与已报道的菊花miR156相比，第14位的碱基也不相同，进化分析显示关系较远。miR156的成熟序列保守性强，其前体序列其它部分差异较大，特别是发夹区。cmo-MIR156c在菊花各器官中存在表达差异，其中根和管状花表达量最高。cmo-MIR156c在拟南芥中异源表达后改变莲座叶数目、分枝数及开花时间等，并影响花和角果的发育。
- Abstract:
- Taking Chrysanthemum ‘Jinba’ and Arabidopsis thaliana as experimental materials，the precursor sequence and secondary structure，evolutionary relationship，expression pattern，growth and development function of Chrysanthemum MIR156c gene were studied by bioinformatics and molecular biology methods，in order to provide reference for the molecular mechanism of Chrysanthemum growth and development.The results showed that the sequence length of the cloned Chrysanthemum MIR156c was 212 bp.cmio-MIR156c could form a typical stem-loop structure，which was specific at the 11th position of the mature sequence.Compared with the reported Chrysanthemum miR156，the 14th base was also different，and evolutionary analysis showed that the relationship was far.The mature sequence of miR156 was highly conserved，and the rest of its precursor sequence was quite different，especially the hairpin region.The expression of cmo-MIR156c was different in various organs of Chrysanthemum，with the highest expression in roots and tubular flowers.The heterologous expression of cmo-MIR156c in Arabidopsis changed the number of rosette leaves，the number of branches and flowering time，and affected the development of flowers and siliques.
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备注/Memo
第一作者简介：胡磊（1996-），男，硕士研究生，研究方向为植物激素与生长发育。E-mail：2694111032@qq.com.责任作者：黄志刚（1979-），男，博士，副教授,现主要从事植物激素与生长发育等研究工作。E-mail：klphzg@163.com.基金项目：国家重点研发计划资助项目(2018YFD1000404);湖南省自然科学基金资助项目(2018JJ2170)。收稿日期：2023-05-19