SUN Pengbo,WANG Zhijun,GE Gentu,et al.Research Progress of Alfalfa Salt and Alkali Stress Tolerance and Mitigation Measures[J].Northern Horticulture,2023,(21):131-137.[doi:10.11937/bfyy.20230885]
紫花苜蓿耐盐碱胁迫与缓减措施的研究进展
- Title:
- Research Progress of Alfalfa Salt and Alkali Stress Tolerance and Mitigation Measures
- 文章编号:
- 1001-0009(2023)21-0131-07
- Keywords:
- alfalfa; saline-alkali stress; molecular biology; mitigation measures
- 分类号:
- S 359
- 文献标志码:
- A
- 摘要:
- 紫花苜蓿是一种重要的豆科牧草,然而,由于紫花苜蓿对盐碱胁迫的反应在遗传和生理上都很复杂,对其耐盐碱胁迫机理研究以及如何缓减盐碱胁迫,充分发挥紫花苜蓿的优势是具有挑战性的。该文基于转录组学、蛋白质组学和代谢组学等分子生物学的研究方法,阐述紫花苜蓿的耐盐碱机理与减缓盐碱胁迫的生理反应,并探讨了紫花苜蓿耐盐碱的机理的应用潜力,以期为盐碱胁迫下紫花苜蓿的耐盐碱机理、缓减盐碱胁迫的损害及紫花苜蓿种植方面提供参考依据。
- Abstract:
- Alfalfa is an important legume forage.However,since the response of alfalfa to salt-alkali stress is both genetically and physiologically complex,it is challenging to study the mechanism of its salt-alkali stress tolerance and how to reduce salt-alkali stress and give full play to its advantages.This study was based on molecular biology methods,such as the transcriptomics,proteomics and metabolomics,expounds the mechanism of salt tolerance of alfalfa and slow the physiological reaction of salinity-alkalinity stress,and discussed the application of the mechanism of salinity tolerance of alfalfa potential,so as to the salt-tolerant mechanism of alfalfa under salinity stress,ease salinity-alkalinity stress damage and alfalfa planting provided reference.
参考文献/References:
[1]SONG Y,LV J,MA Z,et al.The mechanism of alfalfa (Medicago sativa L.) response to abiotic stress[J].Plant Growth Regulation,2019,89:239-249.[2]李丽,王馨,张悦婧,等.非生物胁迫对烟草悬浮细胞胞内和胞外ATP水平的影响[J].植物研究,2023,43(2):179-185.[3]王晓春,杨天辉,王川,等.混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J].中国农学通报,2022,38(19):139-145.[4]张晶,杨树青,郑彦,等.生物措施显著提高农牧交错区盐渍土的暗管排盐效果[J].植物营养与肥料学报,2022,28(12):2299-2309.[5]冯前会,赵于清,吴忧,等.盐碱地苜蓿对断奶羔羊生长发育和健康状况的影响[J].经济动物学报,2022,26(3):172-177,185.[6]ZHANG H H,LI X,GUAN Y P,et al.Physiological and proteomic responses of reactive oxygen species metabolism and antioxidant machinery in mulberry (Morus alba L.) seedling leaves to NaCl and NaHCO3 stress[J].Ecotoxicology and Environmental Safety,2020,193:110259.[7]孙鲁鹏,杨洋,王亚娟,等.有机液体肥对盐碱胁迫下油菜幼苗生理及光合特性的影响[J].北方园艺,2022(8):1-8.[8]刘建新,刘瑞瑞,刘秀丽,等.外源硫化氢对盐碱胁迫下裸燕麦叶片有机酸和激素含量的影响[J].植物研究,2023,43(1):76-89.[9]LI J,ESSEMINE J,SHANG C,et al.Combined proteomics and metabolism analysis unravels prominent roles of antioxidant system in the prevention of alfalfa (Medicago sativa L.) against salt stress[J].International Journal of Molecular Sciences,2020,21(3):909.[10]李中清.褪黑素对混合盐碱胁迫下梭梭幼苗生理特性的影响[J].现代园艺,2023,46(2):16-17,40.[11]王文团.耐盐碱细菌筛选与生物菌肥对盐碱地作物的影响[D].泰安:山东农业大学,2020.[12]孙绍营,王健鸿,王静文,等.盐碱胁迫对细叶百合种子萌发和幼苗生长的影响[J].江苏农业科学,2022,50(24):106-112.[13]侯雪,陈雨洁,李春苗,等.调环酸钙对盐碱胁迫下绿豆苗期生长的调控作用[J].作物杂志,2022,211(6):174-180.[14]胡钰婕,吴玺,王证德,等.盐碱胁迫对马铃薯生理生态特性的影响研究进展[J].热带农业科学,2022,42(12):80-85.[15]ZHANG J T,MU C S.Effects of saline and alkaline stresses on the germination,growth,photosynthesis,ionic balance and anti-oxidant system in an alkali-tolerant leguminous forage Lathyrus quinquenervius[J].Soil Science and Plant Nutrition,2009,55(5):685-697.[16]MUNNS R,DAY DA,FRICKE W,et al.Energy costs of salt tolerance in crop plants[J].New Phytologist,2020,225(3):1072-1090.[17]任怀新,王冬梅,王慧,等.生物炭对盐碱胁迫下黑麦草和紫花苜蓿光合及抗氧化特征的影响[J].农业工程学报,2021,37(17):116-123.[18]ZHU K,ZHANG J,CUI W,et al.Role of heme oxygenase-1 in spermidine-induced alleviation of salt toxicity during alfalfa seed germination[J].Plant and Soil,2014,375:275-287.[19]陈如男,任春元,李贺,等.外源褪黑素通过诱导SlRR基因表达调控番茄盐碱胁迫响应[J].北方园艺,2021(10):1-8.[20]龚远博,胡吉怀,胡丁猛,等.丛枝菌根真菌对盐碱胁迫下杜梨幼苗生长和生理特性的影响[J].西北植物学报,2022,42(8):1320-1329.[21]ZHANG Y,LING H J,WANG D,et al.Foxtail millet CBL4 (SiCBL4) interacts with SiCIPK24,modulates plant salt stress tolerance[J].Plant Molecular Biology Reporter,2017,35:634-646.[22]ZHANG C,BIAN M,YU H,et al.Identification of alkaline stress-responsive genes of CBL family in sweet sorghum (Sorghum bicolor L.)[J].Plant Physiology and Biochemistry,2011,49(11):1306-1312.[23]AN Y,YANG X X,ZHANG L,et al.Alfalfa MsCBL4 enhances calcium metabolism but not sodium transport in transgenic tobacco under salt and saline-alkali stress[J].Plant Cell Reports,2020,39:997-1011.[24]李红,李波,邬婷婷,等.紫花苜蓿耐苏打盐碱相关基因的转录组学分析[J].草地学报,2019,27(4):848-858.[25]DU B,ZHAO W,AN Y,et al.Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco[J].Biology Open,2019,8(9):bio043505.[26]MA J,QIU D,XU L,et al.Overexpression of alfalfa γ-tocopherol methyltransferase (γ-TMT) gene increases salt susceptibility of transgenic Arabidopsis in seed germination[J].Environmental and Experimental Botany,2020,180:104264.[27]坚伟宁.紫花苜蓿MsERF003基因的克隆及其在碳酸盐胁迫响应中的功能分析[D].长春:长春师范大学,2020.[28]CHEN T,YANG Q,ZHANG X,et al.An alfalfa (Medicago sativa L.) ethylene response factor gene,MsERF11,enhances salt tolerance in transgenic Arabidopsis[J].Plant Cell Reports,2012(31):1737-1746.[29]金勋,杨柳,李娜,等.植物逆境生物学中的蛋白质组学技术运用探析[J].现代园艺,2022,45(2):180-182.[30]李晴,沈陈金鑫,薛付冲,等.化学蛋白质组学方法解析天然产物生物合成途径[J].三峡大学学报(自然科学版),2022,44(4):107-112.[31]陈玲玲.敖汉苜蓿小花与种子响应硼胁迫的蛋白质组学与代谢组学分析[D].北京:中国农业大学,2017.[32]XIONG J,SUN Y,YANG Q,et al.Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots[J].Proteome Science,2017,15(1):1-19.[33]LI J,ESSEMINE J,SHANG C,et al.Combined proteomics and metabolism analysis unravels prominent roles of antioxidant system in the prevention of alfalfa (Medicago sativa L.) against salt stress[J].International Journal of Molecular Sciences,2020,21(3):909.[34]MA Q,KANG J,LONG R,et al.Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination[J].Molecular Biology Reports,2017,44:261-272.[35]GAO Y,CUI Y,LONG R,et al.Salt-stress induced proteomic changes of two contrasting alfalfa cultivars during germination stage[J].Journal of the Science of Food and Agriculture,2019,99(3):1384-1396.[36]RAHMAN M A,ALAM I,KIM Y G,et al.Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach[J].Plant Physiology and Biochemistry,2015,89:112-122.[37]刘悦,李青超,兰英,等.植物根际促生菌的筛选、定殖分析及对玉米生长的影响[J].黑龙江农业科学,2023(3):73-78.[38]马欣,成妍,马蓉丽.植物根围促生细菌促生机制研究进展[J].山东农业科学,2019,51(5):148-154.[39]刘冠一,刘艳玲,刘博文,等.含ACC脱氨酶活性的复合菌株提高苜蓿抗盐碱能力研究[J].核农学报,2017,31(5):1022-1028.[40]LIU J,TANG L,GAO H,et al.Enhancement of alfalfa yield and quality by plant growth-promoting rhizobacteria under saline-alkali conditions[J].Journal of the Science of Food and Agriculture,2019,99(1):281-289.[41]李昌宁,李政璇,曹全熙,等.5株植物根际促生菌对紫花苜蓿生长和品质的影响[J].草原与草坪,2018,38(3):29-34.[42]向君亮,唐呈瑞,王佳琦,等.盐碱胁迫下一株促进苜蓿生长的细菌筛选与鉴定[J].干旱地区农业研究,2019,37(2):266-272.[43]代金霞,田平雅,沈聪,等.耐盐植物根际促生菌筛选及促生效应研究[J].生态环境学报,2021,30(5):968-975.[44]张丽辉,赵骥民.Na2CO3胁迫下不同PGPR对紫花苜蓿幼苗生理的影响[J].安徽农业科学,2010,38(32):18045-18047.[45]ANSARI M,SHEKARI F,MOHAMMADI M H,et al.Salt-tolerant plant growth-promoting bacteria enhanced salinity tolerance of salt-tolerant alfalfa (Medicago sativa L.) cultivars at high salinity[J].Acta Physiologiae Plantarum,2019,41:1-13.[46]李宇兴,王海娇,李明鸽,等.产多胺菌鉴定及其对黄瓜幼苗的促生作用研究[J].扬州大学学报(农业与生命科学版),2023,44(1):49-53.[47]LOU Y H,GUAN R,SUN M J,et al.Spermidine application alleviates salinity damage to antioxidant enzyme activity and gene expression in alfalfa[J].Ecotoxicology,2018(27):1323-1330.[48]PALMA F,LPEZ-GMEZ M,TEJERA N A,et al.Involvement of abscisic acid in the response of Medicago sativa plants in symbiosis with Sinorhizobium meliloti to salinity[J].Plant Science,2014,223:16-24.[49]夏关雪莹.盐碱胁迫下苜蓿对亚精胺浸种的生理应答特征[D].沈阳:沈阳农业大学,2019.[50]张茂明,丁俊杰,刘凯,等.盐碱胁迫下外源硅对水稻绥粳306离子平衡调节的复式研究[J].黑龙江农业科学,2022(11):22-26.[51]MENG Y,YIN Q,YAN Z,et al.Exogenous silicon enhanced salt resistance by maintaining K+/Na+ homeostasis and antioxidant performance in alfalfa leaves[J].Frontiers in Plant Science,2020(11):1183.[52]刘慧霞,王康英,郭正刚.不同土壤水分条件下硅对紫花苜蓿生理特性及品质的影响[J].中国草地学报,2011,33(3):22-27.[53]LIU D,LIU M,LIU X L,et al.Silicon priming created an enhanced tolerance in alfalfa (Medicago sativa L.) seedlings in response to high alkaline stress[J].Frontiers in Plant Science,2018(9):716.[54]王小山,顾洪如.外源硅对紫花苜蓿抗盐性的影响[J].江苏农业科学,2009(6):266-269.[55]张昌爱,辛淑荣,王国良,等.不同灌水方式对盐碱地苜蓿生长及土壤水盐动态的影响[J].排灌机械工程学报,2017,35(3):271-276.[56]钱志豪,韩丙芳,刘自婷,等.渗灌对宁夏引黄灌区苜蓿生长性状及水分利用率的影响[J].草业学报,2020,29(3):147-156.[57]郭耀东,程曼,赵秀峰,等.轮作绿肥对盐碱地土壤性质、后作青贮玉米产量及品质的影响[J].中国生态农业学报,2018,26(6):856-864.[58]MBARKI S,SKALICKY M,TALBI O,et al.Performance of Medicago sativa grown in clay soil favored by compost or farmyard manure to mitigate salt stress[J].Agronomy,2020,10(1):94.[59]吕会刚,康俊梅,龙瑞才,等.播种量和行距配置对盐碱地紫花苜蓿草产量及品质的影响[J].草业学报,2019,28(3):164-174.[60]卢发光.种植密度和施氮量对沿海盐碱地紫花苜蓿生长、生理、产量和品质的影响[D].扬州:扬州大学,2022.[61]马先锋,周泉佚,曹志东,等.行距对不同品种紫花苜蓿生长和产量的影响[J].畜牧兽医杂志,2016,35(4):11-14.[62]XIONG X,WEI Y,CHEN J,et al.Transcriptome analysis of genes and pathways associated with salt tolerance in alfalfa under non-uniform salt stress[J].Plant Physiology and Biochemistry,2020,151:323-333.
相似文献/References:
[1]代惠萍,赵 桦,李军超,等.锌对紫花苜蓿生理生化特性的影响及积累能力研究[J].北方园艺,2014,38(10):50.
DAI Hui-ping,ZHAO Hua,LI Jun-chao,et al.Effects of Zn on the Physiological and Biochemical Characteristics and Accumulation Abilities of??Medikago sativa?L.[J].Northern Horticulture,2014,38(21):50.
[2]江超,夏阳,杨克强.盐胁迫下紫花苜蓿苗期根部生长及生理特性研究[J].北方园艺,2014,38(05):68.
JIANG Chao,XIA Yang,YANG Ke-qiang.Research on the Root Physiological Characteristics of Medicago sativa L.Seedling Under Salt Stress[J].Northern Horticulture,2014,38(21):68.
[3]朱华敏,李海梅,杨国锋,等.施肥对紫花苜蓿生产性能的影响[J].北方园艺,2013,37(14):174.
ZHU Hua-min,LI Hai-mei,YANG Guo-feng,et al.Effect of Fertilizer Application on Production Performance of Alfalfa[J].Northern Horticulture,2013,37(21):174.
[4]段亚楠,卢福荣,茹艺,等.紫花苜蓿组织培养高频再生体系的建立[J].北方园艺,2013,37(15):127.
DUAN Ya-nan,LU Fu-rong,RU Yi,et al.Establishment of High Frequency Regeneration System of Medicago sativa[J].Northern Horticulture,2013,37(21):127.
[5]赵九洲,胡立盼,徐志然,等.甜瓜幼苗耐盐碱性及缓解盐碱胁迫γ-氨基丁酸浓度的筛选[J].北方园艺,2014,38(09):1.
ZHAO Jiu-zhou,HU Li-pan,XU Zhi-ran,et al.The Screening of Melon(Cucumis melo L.)Seedling Cultivars for Salt-alkaline Tolerance and the Concentration of γ-aminobutyric Acid Alleviating Salt-alkaline Stress[J].Northern Horticulture,2014,38(21):1.
[6]孔 静,裴宗平,杜 旼,等.水分胁迫下AM真菌对紫花苜蓿生长及抗旱性的影响[J].北方园艺,2014,38(09):179.
KONG Jing,PEI Zong-ping,DU Min,et al.Effects of AM Fungi on Alfalfa Growth and Drought Resistance Under Water Stress[J].Northern Horticulture,2014,38(21):179.
[7]李红,李波,王烨,等.卫星搭载苜蓿叶片愈伤组织对盐碱胁迫的响应[J].北方园艺,2013,37(05):100.
LI Hong,LI Bo,WANG Ye,et al.Alfalfa Leaf Callus on Space Flight Induction and Response on Saline-alkali Stress[J].Northern Horticulture,2013,37(21):100.
[8]公婷婷,冯金朝,薛达元,等.盐碱胁迫对两个品种葡萄光合特性的影响[J].北方园艺,2013,37(19):5.
GONG Ting-ting,FENG Jin-chao,XUE Da-yuan,et al.Influence of Salinity-alkalinity Stress on the Photosynthetic Characteristics of Two Grape Varieties[J].Northern Horticulture,2013,37(21):5.
[9]金文斌,张凡兵.紫花苜蓿生长特性及品质对不同刈割强度的响应[J].北方园艺,2014,38(21):72.
JIN Wen-bin,ZHANG Fan-bing.Response of Quality and Growth Characteristics of Medicago sativa for the Different Intensity of Cutting[J].Northern Horticulture,2014,38(21):72.
[10]李雪芬.氮添加对紫花苜蓿生长特性及光合生理特性的影响[J].北方园艺,2015,39(02):158.[doi:10.11937/bfyy.201502046]
LI Xue-fen.Effect of Nitrogen Addition on Growth and Photosynthetic Physiological Characteristics of Medicago sativa[J].Northern Horticulture,2015,39(21):158.[doi:10.11937/bfyy.201502046]
备注/Memo
第一作者简介:孙鹏波(1996-),男,博士研究生,研究方向为草产品加工理论与技术。E-mail:911795772@qq.com.责任作者:贾玉山(1962-),男,博士,教授,现主要从事牧草加工理论与技术等研究工作。E-mail:jys_nm@sina.com.基金项目:“科技兴盟”行动重点专项资助项目(KJXM-EEDS-2020010);内蒙古自治区科技计划资助项目(2021GG0109);国家自然科学基金资助项目(32260346)。收稿日期:2023-03-20