YANG Lijun,LUO Wei,CUI Chenxu,et al.Optimization of Deproteinization Process and Biological Activity of Adiantum capillus-veneris Polysaccharide by Response Surface Methodology[J].Northern Horticulture,2023,(20):77-85.[doi:10.11937/bfyy.20230239]
响应面优化铁线蕨多糖脱蛋白工艺及生物活性研究
- Title:
- Optimization of Deproteinization Process and Biological Activity of Adiantum capillus-veneris Polysaccharide by Response Surface Methodology
- 文章编号:
- 1001-0009(2023)20-0077-09
- Keywords:
- Adiantum capillus-veneris; polysaccharide; Sevage method; lowering blood sugar; antioxidant
- 分类号:
- TS 241
- 文献标志码:
- A
- 摘要:
- 以铁线蕨为试材，采用单因素试验和响应面试验相结合的优化方法，对铁线蕨多糖脱蛋白工艺进行优化，测定了脱蛋白前后的铁线蕨多糖对α-葡萄糖苷酶的抑制活性与对DPPH自由基的清除能力，以期为铁线蕨多糖的综合利用提供参考依据。结果表明：铁线蕨多糖的最佳脱蛋白工艺条件为多糖溶液与Sevage试剂的体积比为2∶1，脱蛋白时间为10 min，脱蛋白次数为5次，蛋白质脱除率可达73.41%，多糖损失率为25.92%；未脱蛋白的铁线蕨多糖对α-葡萄糖苷酶与对DPPH自由基的IC50值分别为0.31、0.81 mg·mL-1；脱蛋白后的铁线蕨多糖对α-葡萄糖苷酶与对DPPH自由基的IC50值分别为0.26、0.59 mg·mL-1，相较于未脱蛋白分别提高了0.05、0.22 mg·mL-1，表明Sevage法脱除蛋白质可以有效提高铁线蕨多糖抑制α-葡萄糖苷酶的能力与抗氧化能力。
- Abstract:
- Taking Adiantum capillus-veneris as test material,the optimization method of combining single factor experiments and response interviews was used to optimize the deproteinization process of polysaccharides from Adiantum capillus-veneris.The effects of polysaccharides from Adiantum capillus-veneris before and after deproteinization were measured α-glucasidase inhibitory activity of glucosidase and its ability to scavenge DPPH free radicals were expected,in order to provide reference for the comprehensive utilization of polysaccharides from Adiantum capillus-veneris.The results showed that the optimal deproteinization process conditions of Adiantum capillus-veneris polysaccharides were as follows,the volume ratio between polysaccharide solution and Sevage reagent was 2∶1，the deproteinization time was 10 minutes，the deproteinization time was 5 times，the protein removal rate could reach 73.41%，and the polysaccharide loss was 25.92%.The IC50 of undeproteinized Adiantum capillus-veneris polysaccharides for α-glucosidase and DPPH radicals were 0.31,0.81 mg·mL-1，respectively.The IC50 of clematis Adiantum capillus-veneris polysaccharides for α-glucosidase and DPPH radicals were 0.26,0.59 mg·mL-1，respectively，which increased by 0.05,0.22 mg·mL-1 compared with undeproteinized proteins，respectively，which proved that the removal of protein by Sevage method could effectively improve the ability of Adiantum capillus-veneris polysaccharides to inhibit α-glucosidase and antioxidant capacity.
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备注/Memo
第一作者简介：杨立军（1993-），男，硕士，讲师，现主要从事天然产物研究与开发等研究工作。E-mail:yanglijun0417@126.com.责任作者：〖JP2〗董翠（1983-），女，硕士，讲师，现主要从事天然产物研究与开发等工作。E-mail:m18237678036@163.com.基金项目：国家自然科学基金资助项目（32002083）；河南省科技攻关资助项目（222102110247）；河南省重大科技专项资助项目（创新示范专项）（201111310900）；信阳农林学院青年基金资助项目（2019LG011,QN2021061）。收稿日期：2023-01-30