[1]徐启江,黄俊慧,何涛,等.AtWUS过表达诱导分蘖洋葱体细胞胚高频发生[J].北方园艺,2024,(1):24-30.[doi:10.11937/bfyy.20230079]
　XU Qijiang,HUANG Junhui,HE Tao,et al.AtWUS Promotes High Frequency Somatic Embryogenesis Potato Onion（Allium cepa L.var.Aggregatum G.Don）[J].Northern Horticulture,2024,(1):24-30.[doi:10.11937/bfyy.20230079]
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AtWUS过表达诱导分蘖洋葱体细胞胚高频发生

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年1 页码: 24-30 栏目: 出版日期: 2024-01-15
Title:
AtWUS Promotes High Frequency Somatic Embryogenesis Potato Onion（Allium cepa L.var.Aggregatum G.Don）
文章编号:
1001-0009(2024)01-0024-07
作者:
徐启江1; 2; 黄俊慧3; 何涛2; 邢志勇1; 2; 杨安妮1; 黄云彤1; 2
（1.右江民族医学院 生物医药与大健康现代产业学院，广西 百色 533000；2.右江民族医学院 医学检验学院，广西 百色 533000；3.百色市科学技术信息研究所，广西 百色 533000）
Author(s):
XU Qijiang1; 2; HUANG Junhui3; HE Tao2; XING Zhiyong1; 2; YANG Anni1; HUANG Yuntong1; 2
(1.Modern Industrial College of Biomedicine and Great Health，Youjiang Medical University for Nationalities，Baise，Guangxi 533000；2.School of Laboratory Medicine，Youjiang Medical University for Nationalities，Baise，Guangxi 533000；3.Baise Institute of Scientific and Technical Information，Baise，Guangxi 533000)
关键词:
分蘖洋葱; 茎尖外植体; 愈伤组织; 体细胞胚发生; AtWUS基因
Keywords:
potato onion (Allium cepa var.aggregatum Don); shoot apex explants; callus; somatic embryogenesis; AtWUS gene
分类号:
S 633.2
DOI:
10.11937/bfyy.20230079
文献标志码:
A
摘要:
以分蘖洋葱茎尖外植体诱导的愈伤组织为试材，采用农杆菌介导法，将外源基因AtWUS导入分蘖洋葱愈伤组织中，研究AtWUS对分蘖洋葱愈伤组织诱导体细胞胚发生的影响，以期建立分蘖洋葱体细胞胚高频发生体系。结果表明：过表达AtWUS可以显著促进分蘖洋葱愈伤组织的体细胞胚发生率（大约6倍），91%的双极性体细胞胚具有发育形成完整植株能力。RT-PCR分析结果表明，AtWUS在体细胞胚和转基因植株中高丰度表达。
Abstract:
Taking callus induced from shoot apex explants of potato onion (Allium cepa var.aggregatum Don) as test material，the foreign gene AtWUS genetic transformation was carried out by the agrobacterium-mediated transformation method with potato onion callus.The effects of AtWUS on somatic embryogenesis (SE) of potato onion calluswas studied，in order to develop a high-frequency SE regeneration systemvia over-expressing AtWUS from in potato onion callus.The results showed that overexpression of the AtWUS gene incallus promoted，as the percentage of explants giving rise to embryogenic tissues was significantly higher (×6) when AtWUS gene was over-expressed than in the control.Bipolar somatic embryos resulted 91% complete plant regeneration.RT-PCR analysis showed that AtWUS gene was highly expressed in somatic embryos and transgenic plants.

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备注/Memo

第一作者简介：徐启江（1969-），男，博士，教授，现主要从事被子植物分子系统发育遗传学、药用植物功能小分子合成机制与药理学及植物分子育种等研究工作。E-mail：qijiangxu@126.com.责任作者：黄云彤（1971-），女，硕士，教授，现主要从事药用植物功能小分子合成机制与药理学等研究工作。E-mail：01368@ymun.edu.cn.基金项目：广西自然科学基金面上资助项目（2023GXNSFAA026289）；2022年百色市科学研究与技术开发计划资助项目（百科2022002）；2023年第二批百色市科学研究与技术开发计划自筹经费资助项目（百科20232021）。收稿日期：2023-11-10

更新日期/Last Update: 2024-01-22