CUI Hongxin,GUAN Sihui,CHAI Yaqian,et al.Effects of Soil Saline-alkali Stress Degree on Photosynthetic Characteristics of Tomato[J].Northern Horticulture,2023,(17):15-24.[doi:10.11937/bfyy.20225299]
土壤盐碱胁迫程度对番茄光合特性的影响
- Title:
- Effects of Soil Saline-alkali Stress Degree on Photosynthetic Characteristics of Tomato
- 文章编号:
- 1001-0009(2023)17-0015-10
- 关键词:
- 盐碱胁迫; 叶绿素荧光诱导动力学; 盆栽试验; PSⅡ; 电子传递
- Keywords:
- salin-alkali stress; chlorophyll fluorescence induction kinetics; pot experiments; PSⅡ; electron transport
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以C2和C5 2个不同抗性番茄种质为试材，采用盆栽土培的方式，设置3个不同胁迫程度盐碱土处理，以非盐碱胁迫土壤为对照，研究了不同盐碱胁迫程度对2个番茄品种光合气体交换参数，以及对叶片快速叶绿素荧光动力学曲线及参数的影响，以期为进一步全面解析番茄耐盐碱机制和抗盐碱育种提供参考依据。结果表明：盐碱胁迫通过对番茄叶绿素合成的抑制，对光合气体交换参数产生影响，从而降低净光合速率；盐碱胁迫降低了2个番茄品种最大光化学效率、实际光化学效率、光合电子传递速率、光化学淬灭系数；同时盐碱胁迫改变了2个品种番茄OJIP曲线形状。综上可知，盐碱胁迫会导致C2和C5品种PSⅡ反应中心活性、电子传递效率和光化学效率降低，OEC和PSⅡ受体侧受到损伤，叶绿素含量下降，从而使净光合速率降低。在盐碱胁迫条件下，C2品种较C5品种具有较高的PSⅡ反应中心活性和电子传递速率，提高净光合速率，以及通过耗散过量光能，减少盐碱胁迫带来损害，因此生长受抑制程度显著低于C5品种。
- Abstract:
- Taking C2 and C5 tomato germplasm with different resistance as experimental materials，pot experiments were carried out and three saline-alkali soil treatments with different stress degrees were set up.Compared with non-saline-alkali stressed soil，the effects of different saline-alkali stress degrees on photosynthetic gas exchange parameters of two tomato varieties，as well as the rapid chlorophyll fluorescence kinetic curve and parameters of leaves were studied，in order to provide reference for further comprehensive analysis of tomato salt-alkali tolerance mechanism and salt-alkali resistance breeding.The results showed that saline-alkali stress reduced the net photosynthetic rate and affected the parameters of light and gas exchange by inhibiting the synthesis of chlorophyll in tomato.Saline-alkali stress reduced the maximum photochemical efficiency，actual photochemical efficiency，photosynthetic electron transfer rate and photochemical quenching coefficient of the two tomato varieties.At the same time，saline-alkali stress also changed the shape of OJIP curve of two tomato varieties.To sum up，saline-alkali stress would reduce the activity，electron transfer efficiency and photochemical efficiency of PSⅡ reaction center of C2 and C5 varieties，harm OEC and PSⅡ receptor side，reduce chlorophyll content，and thus reduce the net photosynthetic rate.In case of saline-alkalitress，C2 variety had higher PSⅡ reaction center activity and electron transfer rate than C5 variety，improved the net photosynthetic rate，and reduced the damage caused by saline-alkali stress by dissipating excess light energy，so the growth inhibition degree was significantly lower than C5 variety.
参考文献/References:
［1］ 朱金方，刘京涛，陆兆华,等.盐胁迫对中国柽柳幼苗生理特性的影响［J］.生态学报，2015，35(15)：5140-5146.［2］ ZELM E V，ZHANG Y，TESTERINK C.Salt tolerance mechanisms of plants［J］.Annual Review of Plant Biology，2020，71(1)：507-513.［3］ 郭家鑫，鲁晓宇，陶一凡，等.盐碱胁迫对棉花生长和养分吸收的影响［J］.干旱地区农业研究,2022，40(4)：23-32,59.［4］ SHI D，SHENG Y.Effect of various salt-alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors［J］.Environmental and Experimental Botany，2004，54(1)：54-75.［5］ 李学孚，倪智敏，吴月燕，等.盐胁迫对‘鄞红’葡萄光合特性及叶片细胞结构的影响［J］.生态学报,2015，35(13)：4436-4444.［6］ YANG J Y，ZHENG W，TIAN Y，et al.Effects of various mixed salt-alkaline stresses on growth，photosynthesis，and photosynthetic pigment concentrations of medicago ruthenica seedlings［J］.Photosynthetica，2011，49(2)：275-284.［7］ KALAJI H M，JAJOO A，OUKARROUM A，et al.Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions［J］.Acta Physiologiae Plantarum，2016，38(4)：38-42.［8］ 刘卫国，丁俊祥，邹杰，等.NaCl对齿肋赤藓叶肉细胞超微结构的影响［J］.生态学报,2016，36(12)：3556-3563.［9］ GRIEVE C M，LESCH S M，MAAS E V，et al.Leaf and spikelet primordia initiation in salt-stressed wheat［J］.Crop Science，1993，33(6)：1286-1294.［10］张继峯，王振华，张金珠，等.盐碱胁迫对滴灌加工番茄生理生长和干物质积累的影响［J］.水土保持学报,2019，33(1)：270-276. ［11］严衍禄，刘心生.叶绿素测定方法的研究［J］.北京农业大学学报,1982(2)：53-67.［12］温泽林.外源GSH介导NO调控番茄幼苗盐适应性研究［D］.石河子:石河子大学，2018.［13］杨建军，张国斌，郁继华,等.盐胁迫下内源NO对黄瓜幼苗活性氧代谢和光合特性的影响［J］.中国农业科学，2017，50(19)：3778-3788.［14］SCHANSKER G，SRIVASTAVA A，GOVINDJE E，et al.Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves［J］.Functional Plant Biology，2003，30(7)：785-796.［15］朱延凯，王振华，李文昊.不同盐胁迫对滴灌棉花生理生长及产量的影响［J］.水土保持学报，2018，32(2)：298-305.［16］SHARKEY T D，IMAI K，FARQUHAR G D，et al.A direct confirmation of the standard method of estimating intercellular partial pressure of CO2［J］.Plant Physiology，1982，69(3)：657-659.［17］张春梅，邹志荣，黄志，等.外源亚精胺对干旱胁迫下不同品种番茄幼苗光合作用的影响［J］.干旱地区农业研究,2010，28(3)：182-187.［18］王素平，郭世荣，胡晓辉，等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响［J］.江西农业大学学报,2006(1)：32-38.［19］周静.藜麦对NaHCO3胁迫适应性机制研究［D］.长春:东北师范大学，2017.［20］BAKER N R，ROSENQVIST E.Applications of chlorophyll fluorescence can improve crop production strategies：An examination of future possibilities［J］.Journal of Experimental Botany，2004，55(403)：1607-1621.［21］吴鹏，吕剑，郁继华，等.褪黑素对盐碱复合胁迫下黄瓜幼苗光合特性和渗透调节物质含量的影响［J］.应用生态学报，2022，33(7)：1901-1910.［22］邵瑞鑫，李蕾蕾，郑会芳，等.外源一氧化氮对干旱胁迫下玉米幼苗光合作用的影响［J］.中国农业科学,2016，49(2)：251-159. ［23］WANG L Z，WANG L M，XIANG H T，et al.Relationship of photosynthetic efficiency and seed-setting rate in two contrasting rice cultivars under chilling stress［J］.Photosynthetica，2016，54(4)：581-588.［25］LAZAR D.The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light［J］.Functional Plant Biology，2006，33(1)：9-30.［26］陆思宇，杨再强，张源达,等.高温条件下光周期对鲜切菊花叶片光合系统荧光特性的影响［J］.中国农业气象,2020，41(10)：632-643.［27］李鹏民.快速叶绿素荧光诱导动力学在植物逆境生理研究中的应用［D］.泰安:山东农业大学，2007.
相似文献/References:
[1]赵九洲,胡立盼,徐志然,等.甜瓜幼苗耐盐碱性及缓解盐碱胁迫γ-氨基丁酸浓度的筛选[J].北方园艺,2014,38(09):1.
ZHAO Jiu-zhou,HU Li-pan,XU Zhi-ran,et al.The Screening of Melon（Cucumis melo L.）Seedling Cultivars for Salt-alkaline Tolerance and the Concentration of γ-aminobutyric Acid Alleviating Salt-alkaline Stress[J].Northern Horticulture,2014,38(17):1.
[2]李红,李波,王烨,等.卫星搭载苜蓿叶片愈伤组织对盐碱胁迫的响应[J].北方园艺,2013,37(05):100.
LI Hong,LI Bo,WANG Ye,et al.Alfalfa Leaf Callus on Space Flight Induction and Response on Saline-alkali Stress[J].Northern Horticulture,2013,37(17):100.
[3]公婷婷,冯金朝,薛达元,等.盐碱胁迫对两个品种葡萄光合特性的影响[J].北方园艺,2013,37(19):5.
GONG Ting-ting,FENG Jin-chao,XUE Da-yuan,et al.Influence of Salinity-alkalinity Stress on the Photosynthetic Characteristics of Two Grape Varieties[J].Northern Horticulture,2013,37(17):5.
[4]李 瑞 昌１,曹 帮 华 ２.盐 碱 胁 迫 对 两 种 地 被 竹 生 长 指 标 的 影 响[J].北方园艺,2011,35(13):0.[doi:10.11937/bfyy.2011013029]
.Ｔ ｈ ｅ Ｅ ｆ ｆ ｅ ｃ ｔ ｓ ｏ ｆ Ｓ ａ ｌ ｉ ｎ ｅ － ａ ｌ ｋ ａ ｌ ｉ ｓ ｔ ｒ ｅ ｓ ｓ ｏ ｎ Ｇ ｒ ｏ ｗ ｔ ｈ Ｉ ｎ ｄ ｅ ｘ ｏ ｆ ｔ ｈ ｅ Ｔ ｗ ｏ Ｂ ａ ｍ ｂ ｏ ｏ Ｖ ａ ｒ ｉ ｅ ｔ ｉ ｅ ｓ[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.2011013029]
[5]王 连 君,王 铭,冯 玉 才.盐碱胁迫对山葡萄光合特性的影响[J].北方园艺,2008,32(01):0.[doi:10.11937/bfyy.200801014]
W ANG Lian -jun,WANG Ming,FENG Yu -cai.Effect of Saline -Alkali Stress on Photosynthesis Characteristic of VitisAmurensis Rupr[J].Northern Horticulture,2008,32(17):0.[doi:10.11937/bfyy.200801014]
[6]王 连 君,王 铭,冯 玉 才.盐 碱 胁 迫 对 山 葡 萄 的 危 害[J].北方园艺,2007,31(11):0.[doi:10.11937/bfyy.2007011006]
W ANG Lian - jun,WANG Ming,FENG Yu - cai.The Harm of Saline - Alkali Stress on Vitis Amurensis Rupr[J].Northern Horticulture,2007,31(17):0.[doi:10.11937/bfyy.2007011006]
[7]董园园１,李晓薇,姚娜,等.盐碱胁迫下羊草转录因子的转录组分析[J].北方园艺,2017,41(07):115.[doi:10.11937/bfyy.201707024]
DONG Yuanyuan,LI Xiaowei,YAO Na,et al.Transcription Factor Identification and Analysis in Leymus chinensis Transcriptome[J].Northern Horticulture,2017,41(17):115.[doi:10.11937/bfyy.201707024]
[8]刘莹,徐关印.盐碱胁迫下黄瓜丙二醛含量及有机硅的缓解效应[J].北方园艺,2017,41(22):1.[doi:10.11937/bfyy.20171212]
LIU Ying,XU Guanyin.Malondialdehyde Content in Cucumber Under Neutral-alkaline Salt Stress and Mitigative Effect of Organic Silicon[J].Northern Horticulture,2017,41(17):1.[doi:10.11937/bfyy.20171212]
[9]黄修梅,郝丽珍,惠霖,等.复合盐碱胁迫对蒙古高原野韭种子萌发的影响[J].北方园艺,2018,42(01):1.[doi:10.11937/bfyy.20171063]
HUANG Xiumei,HAO Lizhen,HUI Lin,et al.Effects of Salt-alkali Mixed Stresses on Seed Germination of Allium ramosum From Mongolia Plateau[J].Northern Horticulture,2018,42(17):1.[doi:10.11937/bfyy.20171063]
[10]徐雯,刘芳,裴毅,等.盐碱胁迫对虞美人种子萌发的影响[J].北方园艺,2018,42(10):97.[doi:10.11937/bfyy.20172889]
XU Wen,LIU Fang,PEI Yi,et al.Effect of Salt Alkali Stress on Seed Germination of Papaver rhoeas L.[J].Northern Horticulture,2018,42(17):97.[doi:10.11937/bfyy.20172889]
备注/Memo
第一作者简介：崔洪鑫（1998-），男，硕士研究生，研究方向为设施蔬菜逆境生理。E-mail：c18899535650@163.com.责任作者：刁明（1968-），男，博士，教授，现主要从事园艺作物生理生态信息农业等研究工作。E-mail：diaoming@shzu.edu.cn.基金项目：国家自然科学基金-新疆联合基金重点资助项目（U1903202）。收稿日期：2022-12-30