WANG Hong,ZHENG Xuan,KANG Chao,et al.Effects of Repeated Subcultures on Mycelial Characteristics andTTC-dehydrogenase Activity of Morchella sextelata[J].Northern Horticulture,2023,(11):123-128.[doi:10.11937/bfyy.20223523]
羊肚菌继代培养对菌丝特性及TCC-脱氢酶活性的影响
- Title:
- Effects of Repeated Subcultures on Mycelial Characteristics andTTC-dehydrogenase Activity of Morchella sextelata
- 文献标志码:
- A
- 摘要:
- 以六妹羊肚菌为试材，采用继代培养方法，研究了继代培养次数对菌丝的生长特性和TCC-脱氢酶活性的影响，以期为继代培养次数与菌种退化关系研究提供参考依据。结果表明：不同培养方式下，随着继代次数的增加，菌丝生长速率、生物量、TTC-脱氢酶活性均呈下降趋势，要保持较高酶活性和菌丝活力，继代次数应在5次以内，菌丝萌发快、粗壮、前端辐射状，TCC-脱氢酶活性高，且不同菌种培养方式对TTC-脱氢酶活性影响显著，要充分考虑种性特征与生产方式关系。
- Abstract:
- Taking Morchella sextelata as the test material,the effects of mycelial characteristics and TTC-dehydrogenase activity were investigated by repeated subculture,in order to provide reference for the relationship between repeated subculture and strain degradation.The results showed that under different cultivation conditions，the growth rate of mycelial，biomass and TTC-dehydrogenase activity decreased with repeated subculture increased.In order to maintain high enzyme activity and mycelial activity，repeated subculture in five times，and that mycelial germination was fast，thick and radiation in front.In addition，TTC-dehydrogenase activity was strongly and significantly affected by different methods of strain culture，and the relationship between species characteristics and mode of production should be fully considered.
参考文献/References:
[1]吴兴亮,卯晓岚,图力古尔,等.中国药用真菌[M].北京:科学出版社,2013.[2]贺新生.羊肚菌生物学基础、菌种分离制作与高产栽培技术[M].北京:科学出版社,2017.[3]李华,包海鹰,李玉.羊肚菌研究进展[J].菌物研究,2004(4):53-60.[4]龙正海.羊肚菌的研究及其应用开发前景[J].中国生化药物杂志,1997(3):160-162.[5]刘娜,宋莹,张敏,等.香菇继代培养对菌丝特性和显微结构的影响[J].北方园艺,2022(3):118-124.[6]刘伟,张亚,蔡英丽.我国羊肚菌产业发展的现状及趋势[J].食药用菌,2017,25(2):77-83.[7]刘伟,何培新.高羊肚菌菌株老化过程形态学研究[Z].贵阳，2014.[8]钱可晴,成盼盼,范君佩,等.羊肚菌菌种继代培养代数与菌种退化关系[J].菌物研究,2021,19(4):277-284.[9]康超,曾维军,郑旋,等.羊肚菌菌丝体TTC-脱氢酶及胞外酶活性变化[J].北方园艺,2022(5):119-124.[10]刘桂娟,姚方杰.食用菌菌种质量检测方法概述[Z].杭州，2008.[11]林清泉,丘雪红,郑壮丽,等.蛹虫草退化菌株的特征研究[J].菌物学报,2010,29(5):670-677.[12]沈业寿,卫青,张峻岭.美味侧耳子实体组织的细胞活力[J].中国食用菌,1995(6):33-35.[13]李红,张敏,肖千明,等.利用TTC-脱氢酶还原法快速检测黑木耳菌丝活力的研究[J].食用菌,2016(4):10-12.
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备注/Memo
第一作者简介：王虹（1976-），女，本科，助理工程师，现主要从事生物工程等研究工作。E-mail：279445802@qq.com.责任作者：康超（1987-），男，硕士，高级工程师，现主要从事食药用菌菌种研发、栽培及加工等研究工作。E-mail：464286916@qq.com.基金项目：贵州省科技计划资助项目（黔科合支撑[2022]114,黔科合服企[2019]4007-6）。收稿日期：2022-08-28