YUE Wansong,HUA Rong,SUN Dafeng,et al.Biological Characteristics and Domestic Cultivation of Lepista nuda[J].Northern Horticulture,2023,(06):112-119.[doi:10.11937/bfyy.20223118]
紫丁香蘑生物学特性及驯化栽培
- Title:
- Biological Characteristics and Domestic Cultivation of Lepista nuda
- 文献标志码:
- A
- 摘要:
- 以采自云南省嵩明县的野生紫丁香蘑为试验材料，采用碳源、氮源、pH和温度4个因素进行单因素试验，研究了不同培养条件对菌丝生长的影响。同时，在对其菌丝体最适培养条件研究结果的基础上，采用覆土栽培法对其进行驯化栽培研究，以期为紫丁香蘑的人工栽培提供参考依据。结果表明：菌丝体最适生长温度为25 ℃，最适pH为8.0，最适碳源为麦芽糖，最适氮源为蛋白胨，在以麦秸、棉籽壳、木屑和麸皮为栽培料时，可获得子实体。
- Abstract:
- A wild Lepista nuda collected from Songming area,Yunnan Province,was used as test material.The effects of carbon source,nitrogen source,pH and temperature on growth of the mycelium were investigated by single factor test to investigate effects of different culture conditions on the growth of mycelia.At the same time,based on the results of the study on the optimal culture conditions of the mycelium,the domestication cultivation of the fungus were studied by using soil covering cultivation method,in order to provide reference for the artificial cultivation of Lepista nuda.The results showed that the optimal growth temperature of the mycelium was 25 ℃,the optimal pH of growth was 8.0,the optimal carbon source and nitrogen source were maltose and peptone,respectively.The fruiting body could form in the substrate composed of cottonseed shell,sawdust and bran.
参考文献/References:
[1]李挺,宋斌,林群英,等.我国香蘑属真菌研究进展[J].安徽农业科学,2011,39(13):7579-7581.[2]桂明英,马绍斌,郭相,等.西南大型真菌(Ⅰ)[M].上海:上海科学技术文献出版社,2016.[3]OUZOUNI P K,PETRIDIS D,ROLLER W,et al.Nutritional value and metal content of wild edible mushrooms collected from West Macedonia and Epirus，Greece[J].Food Chemistry,2009,115(4):1575-1580.[4]云南省梳理出227种野生食用菌和14种条件可食野生菌[J].食药用菌,2019(2):128.[5]图力古尔,李玉.中国名蘑100[J].菌物研究,2012,10(3):154-157,172.[6]周峰,王瑞娟,李玉,等.珍稀食药用菌紫丁香蘑的研究进展[J].食用菌学报,2010,17(4):79-83.[7]戴玉成,杨祝良.中国药用真菌名录及部分名称的修订[J].菌物学报,2008,27(6):801-824.[8]DULGER B,ERGUL C C,GUCIN F.Antimicrobial activity of the macrofungus Lepista nuda[J].Fitoterapia,2002,73(7):695-697.[9]庄司当,王波.紫丁香蘑林地栽培试验[J].国外农学:国外食用菌,1989(4):1.[10]戴彩云,催晓明.茶园自生食用菌紫丁香蘑的驯化栽培[J].贵州茶叶,1994,1(1):24-26.[11]卯晓岚,文华安,庄文颖,等.中国大型真菌[M].郑州:河南科学技术出版社,2000.[12]余金凤,陈正启,周汐,等.云南变绿红菇的遗传多样性与群体遗传分化研究[J].食药用菌,2020,28(3):178-184.[13]朱峥,张雷,徐济责.左家保护区的野生紫丁香蘑生物学特性[J].东北林业大学学报,2015,43(8):25-28.[14]崔婉茹,郭普宇,李冰,等.不同碳氮源对紫丁香蘑菌丝生长的对比研究[J].中国林副特产,2019(3):4-6.[15]赵秉浩,刘虹,郭丽杰,等.三种香蘑属野生食用菌菌株的培养条件优化[J].微生物学通报,2019,46(9):2445-2456.[16]胡会泽,图力古尔,张锁峰.五台山“台蘑”资源调查[J].菌物研究,2020,18(1):10-19.[17]罗正明,刘秀丽,贾艳青,等.四种五台山野生食用菌蛋白质营养价值评价[J].食品工业科技,2015,36(2):349-354.[18]胡会泽.五台山食药用真菌资源及“台蘑”的研究[D].长春：吉林农业大学,2020.[19]魏海莲,刘志曦,张婉洁,等.云南昭通野生紫丁香蘑群体的遗传多样性分析[J].北方园艺,2015(17):132-136.[20]邢鹏杰,高婷婷,马世玉,等.紫丁香蘑菌丝纯培养物的分类鉴定及最佳培养基筛选[J].分子植物育种,2017,15(12):5231-5234.[21]吉建成,赵林,王同海,等.一株野生紫丁香蘑的分离纯化与鉴定[J].青岛农业大学学报(自然科学版),2019,36(3):183-187.[22]周峰,王瑞娟,李玉,等.珍稀食药用菌紫丁香蘑的研究进展[J].食用菌学报,2010,17(4):79-83.[23]姜雪,庞惟俏,杨洋,等.两株野生紫色香蘑菌株的鉴定[J].北方园艺,2016(22):152-156.[24]唐平华,杨杰,黄亿,等.一种紫丁香蘑种植方法[P].中国:CN110679389B,2020-01-14.[25]蒋洪久,李朝云,黄亿,等.高粱秸秆栽培紫丁香蘑技术探索[J].现代农机,2022(3):104-105.[26]赵辉,孙传齐,徐德,等.花脸香蘑新品种‘达香蘑1号’[J].园艺学报,2021,48(S2):2859-2860.[27]唐庆明,邹莉,孙少辉,等.灰紫香蘑的分离纯化及ITS序列鉴定[J].安徽农业科学,2014,42(14):4192-4193,4201.
相似文献/References:
[1]关志华,王忠红.三十四份樱桃番茄种质材料耐低温性鉴定[J].北方园艺,2014,38(13):24.
GUAN Zhi-hua,WANG Zhong-hong.Cold Tolerance Appraisal on Seed of 34 Cherry Tomato Germplasm[J].Northern Horticulture,2014,38(06):24.
[2]鲁海菊,董梅,崔同敏,等.枇杷内生真菌多样性初步研究[J].北方园艺,2014,38(04):100.
LU Hai-ju,DONG Mei,CUI Tong-min,et al.Preliminary Study on Diversity of Endophytic Fungi From Eriobotrya japonica[J].Northern Horticulture,2014,38(06):100.
[3]董平,赵培宝,任爱芝.蝴蝶兰灰霉病菌的分离鉴定[J].北方园艺,2014,38(04):109.
DONG Ping,ZHAO Pei-bao,REN Ai-zhi.Isolation and Identification of Botrytis cinerea?in Phalaenopsis[J].Northern Horticulture,2014,38(06):109.
[4]祁鹤兴,胡美娟,王 丽,等.苦豆子内生拮抗放线菌的筛选及活性菌株鉴定[J].北方园艺,2014,38(08):86.
QI He-xing,HU Mei-juan,WANG Li,et al.Screening and Identification of Antagonistic Endophytic Actinomycetes Isolated from Sophora alopecuroides L.[J].Northern Horticulture,2014,38(06):86.
[5]蒋 卉,朱丽霞,郭东起.冬枣采后黑腐病病原菌分离鉴定及控制研究[J].北方园艺,2014,38(09):133.
JIANG Hui,ZHU Li-xia,GUO Dong-qi.Study on the Isolation,Identification and Control of Black Mould Pathogen of Winter Jujube After Postharvest[J].Northern Horticulture,2014,38(06):133.
[6]欧立军,孙海军.基于ITS序列的蔷薇纲二十六个物种遗传多样性分析[J].北方园艺,2013,37(11):94.
OU Li-jun,SUN Hai-jun.Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences[J].Northern Horticulture,2013,37(06):94.
[7]王瑞虎,关 鑫,陈秀玲,等.番茄灰霉病菌的鉴定及系统发育树分析[J].北方园艺,2013,37(06):127.
WANG Rui-hu,GUAN Xin,CHEN Xiu-ling,et al.Identification and Phylogenetic Tree Analysis of Tomato Gray Mould Pathogen[J].Northern Horticulture,2013,37(06):127.
[8]肖淑芹,白 戈,姜晓颖,等.沈阳地区土壤木霉菌分类鉴定[J].北方园艺,2013,37(23):129.
XIAO Shu-qin,BAI Ge,JIANG Xiao-ying,et al.Identification and Classification of Trichoderma spp. in Shenyang Area[J].Northern Horticulture,2013,37(06):129.
[9]徐丽鸣,辛 焱,徐 飞.菜豆种质资源色泽性状鉴定与相关性分析[J].北方园艺,2012,36(18):63.
XU Li-ming,XIN Yan,XU Fei.Seed Color Character Identification and Correlation Analysis on Snap Bean Germplasm Resources[J].Northern Horticulture,2012,36(06):63.
[10]宋敏丽,温祥珍,李亚灵,等.六个番茄品种的耐热性鉴定[J].北方园艺,2012,36(21):5.
SONG Min-li,WEN Xiang-zhen,LI Ya-ling,et al.Heat Tolerance Identification of Six Tomato Varieties[J].Northern Horticulture,2012,36(06):5.
备注/Memo
第一作者简介：岳万松（1990-），男，硕士，助理研究员，现主要从事食用菌育种栽培等研究工作。E-mail：459440031@qq.com.责任作者：刘绍雄（1987-），男，硕士，副研究员，现主要从事食用菌资源开发与菌种选育及栽培技术等研究工作。E-mail:305475365@qq.com.基金项目：云南省科技人才与平台计划资助项目（202005AE160001）；云南省乡村振兴科技专项资助项目（202204BP090012）；云南省重大科技专项计划资助项目（202002AE320003)。收稿日期：2022-07-28