YANG Ju,MU Hongmei,ZHANG Xiqi.Effects of Exogenous Nitric Oxide on Physiological Indexes of Ornamental Lagenaria siceraria Seedlings Under Salt Stress[J].Northern Horticulture,2023,(02):64-71.[doi:10.11937/bfyy.20222208]
外源一氧化氮对盐胁迫下观赏葫芦幼苗生理指标的影响
- Title:
- Effects of Exogenous Nitric Oxide on Physiological Indexes of Ornamental Lagenaria siceraria Seedlings Under Salt Stress
- Keywords:
- ornamental gourd; salt stress; sodium nitroprusside; photosynthetic characteristics; physiological characteristics
- 文献标志码:
- A
- 摘要:
- 以‘亚腰葫芦’为试材，采用喷施85 mmol?L-1 盐溶液的处理方法，研究了不同浓度硝普钠（0.01、0.05、0.10、0.25、0.50 mmol?L-1）对观赏葫芦盐胁迫的缓解作用，以期为外源一氧化氮缓解盐胁迫对葫芦幼苗的伤害提供参考依据。结果表明：硝普钠浓度为 0.10 mmol?L-1 时，显著提高了NaCl胁迫下葫芦的净光合速率、蒸腾速率、气孔导度，促进了叶绿素含量、可溶性糖含量、抗氧化物酶（SOD、APX、CAT）活性的升高，提高了葫芦的株高、干鲜质量、根系根长（Len）、表面积（SA）、体积（Vol）及根尖数（NTips）以及酮类化合物、酚类化合物、木质素含量。随着硝普钠浓度逐渐提高，这些指标呈现出先上升后下降的趋势。0.10 mmol?L-1硝普钠显著降低了游离脯氨酸含量，降低了丙二醛（MDA）〖JP+1〗积累，维持了细胞膨压，改善了细胞与环境的渗透调节。光合荧光参数的相关性分析，Pn、E、Gs、Fm、Fv/Fm、PI abs、psi(Eo)等 7 个参数之间呈正相关，随着硝普钠浓度的升高呈现先升后降的趋势，Fo、DIo/RC 等 2 个参数之间呈负相关，随着硝普钠浓度的提高呈现先降后升的趋势。综上，0.10 mmol?L-1硝普钠溶液为缓解葫芦盐胁迫的最佳浓度。
- Abstract:
- ‘Yayaohulu’ was used as the experimental material，and the treatment method of 85 mmol?L-1 salt stress was used to study the alleviating effects of different concentrations of sodium nitroprusside (0.01，0.05，0.10，0.25，0.50 mmol? L-1) on salt stress of ornamental gourd，in order to provide reference for exogenous nitric oxide to alleviate the damage of salt stress to gourd seedlings.The results showed that when the concentration of sodium nitroprusside was 0.10 mmol?L-1，the net photosynthetic rate，transpiration rate and stomatal conductance of Lagenaria siceraria under NaCl stress were significantly increased，the chlorophyll content and soluble sugar content were promoted，the activities of antioxidant enzymes (SOD，APX and CAT) were promoted，the plant height，dry and fresh welight，root length (Len)，surface area (SA)，volume (Vol) and root tip number (NTips) of Lagenaria siceraria were increased，and the contents of ketone compounds，phenolic compounds and lignin were increased.With the gradual increase of sodium nitroprusside concentration，these indexes showed a trend of first increase and then decrease.0.10 mmol?L-1 sodium nitroprusside significantly decreased free proline content，decreased malondialdehyde (MDA) accumulation，maintained cell swelling pressure and improved osmotic regulation of cells and the environment.Correlation analysis of photosynthetic fluorescence parameters，Pn，E，Gs，Fm，Fv/Fm，PI abs，psi (Eo) and other seven parameters were positively correlated，with the increase of nitrate concentration showed a trend of first increased and then decreased，Fo，DIo/RC and other two parameters were negatively correlated，with the increasing of sodium nitroprusside concentration showed a trend of first decreased and then increased.
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备注/Memo
第一作者简介：杨菊（1997-），女，硕士研究生，研究方向为植物生理生化。E-mail：1969378813@qq.com.责任作者：穆红梅（1974-），女，博士，副教授，现主要从事园林植物生物技术、园林植物种质资源研究与利用、园林植物分子生物学等研究工作。E-mail：652864242@qq.com.基金项目：山东省自然科学基金资助项目（ZR2020MC031）。收稿日期：2022-05-29