GAO Pei,GUO Jiahui,HE Yongchao,et al.Isolation and Identification of Four Pseudomonas strains From Hippophae rhamnoides subsp.sinensis Rousi and Comparison of Their Growth Promoting Ability[J].Northern Horticulture,2023,(01):69-75.[doi:10.11937/bfyy.20221622]
中国沙棘根际四株假单胞菌的分离、鉴定及促生能力的比较
- Title:
- Isolation and Identification of Four Pseudomonas strains From Hippophae rhamnoides subsp.sinensis Rousi and Comparison of Their Growth Promoting Ability
- Keywords:
- wild Hippophae rhamnoides subsp.sinensis Rousi; rhizosphere microorganisms; identification of strains; growth promoting bacteria
- 文献标志码:
- A
- 摘要:
- 以青海野生中国沙棘根际土为试材，从中筛选出具有一定促生能力的菌株，利用传统培养法对野生中国沙棘根际促生菌进行分离，利用平板划线法对菌株进行分纯。通过形态特征、生理生化试验和16S rDNA对分离菌株进行种属鉴定，运用溶磷法对菌株的解磷能力进行测定，用原子吸收仪进行解钾能力的测定，以期为挖掘具有潜在应用价值的高效根际促生菌株创造条件。结果表明：根据生理生化和16S rDNA分子鉴定获得4株分离菌株，4株菌归属于假单胞菌属，能力测定结果显示K7溶解有机磷半径为13.71 mm，K28溶解无机磷半径为7.62 mm，K39解钾率为14.33 μg?mL-1。所得菌株中的3株分别是高效的解有机磷、解无机磷和解钾菌株，表明3株菌株制成混合菌剂可作为开发高效菌肥的潜在材料。
- Abstract:
- Taking Qinghai wild Hippophae rhamnoides subsp.sinensis Rousi rhizosphere soil as material,strains with certain growth promoting ability were screened out.The rhizosphere growth promoting bacteria of wild Hippophae rhamnoides subsp.sinensis Rousi were isolated by traditional culture method,and the strains were purified by plate scribing method,in order to create conditions for excavating high-efficiency rhizosphere growth promoting strains with potential application value.The results showed that morphological characteristics,physiological and biochemical tests,and 16S rDNA,the phosphorus removal capacity of the isolated strains was determined by the phosphorus removal method,and the potassium removal capacity was determined by the atomic absorption meter.Four strains of bacteria were isolated.According to the physiological,biochemical and 16S rDNA molecular identification results,the four strains belonged to Pseudomonas.The ability test results showed that the radius of K7 dissolved organic phosphorus was 13.71 mm,the radius of K28 dissolved inorganic phosphorus was 7.62 mm,and the potassium dissolution rate of K39 was 14.33 μg?mL-1.The three strains obtained were efficient strains for dissolving organic phosphorus,inorganic phosphorus and potassium respectively,indicating that the three strains could be used as potential materials for developing high-efficiency bacterial fertilizer.
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备注/Memo
第一作者简介：高佩（1996-），女，硕士研究生，研究方向为植物资源开发利用。E-mail：1639245486@qq.com.责任作者：马玉花（1978-），女，博士，教授，硕士生导师，现主要从事森林培育理论与技术、植物资源开发利用等研究工作。E-mail：qhxnmyh@163.com.基金项目：国家自然科学基金资助项目(31660071)；青海省科技厅资助项目(2017-ZJ-734)；2020年青海省“高端创新人才千人计划”资助项目。收稿日期：2022-04-20