YANG Zhitao,ZHANG Yanping,ZHU Libao,et al.Exogenous Humic Acid Effects on Muskmelon Seedling Cold Resistance[J].Northern Horticulture,2023,(02):25-31.[doi:10.11937/bfyy.20221391]
外源腐殖酸灌根对甜瓜幼苗抗冷性的影响
- Title:
- Exogenous Humic Acid Effects on Muskmelon Seedling Cold Resistance
- Keywords:
- muskmelon; seedlings; humic acid; the cold resistance
- 文献标志码:
- A
- 摘要:
- 以厚皮甜瓜“玉菇”为试材，待幼苗长至两叶一心时，分别用200、300、400、800 mg?L-1腐殖酸进行灌根；四叶一心时，采用昼/夜温度为10 ℃/5 ℃进行低温处理，测定甜瓜幼苗的株高、茎粗、鲜质量、光合荧光、根系活力、丙二醛(MDA)、电解质渗透率以及抗氧化酶活性，研究了在低温胁迫下不同浓度腐殖酸处理对甜瓜幼苗生长及生理指标的影响，以期为腐殖酸在甜瓜抗冷应用上提供参考依据。结果表明：腐殖酸处理显著提高了幼苗在低温胁迫条件下的根系活力、叶绿素含量和净光合速率，显著降低了低温胁迫条件下幼苗细胞质膜透性和 MDA 含量，提高了抗氧化酶（超氧化物歧化酶、过氧化物酶）活性，且300 mg?L-1处理均达到最大值。综上所述，不同浓度腐殖酸处理对低温胁迫下甜瓜幼苗的光合特性以及生长、生理指标差异显著，其中以腐殖酸浓度为300 mg?L-1处理效果最佳。
- Abstract:
- The thick-skinned muskmelon ‘Jade Mushroom’ was used as the test material，and the method of root irrigation with 200,300,400 mg?L-1 and 800 mg?L-1 humic acid was used.When the four-leaves peroid，the day/night temperature was 10 ℃/5 ℃ for low temperature treatment.By measuring the plant height，stem diameter，fresh weight，photosynthetic fluorescence，root activity，malondialdehyde，electrolyte permeability and antioxidant enzyme activity of melon seedlings，the effects of different concentrations of humic acid on the growth and growth of melon seedlings under low temperature stress were studied，in order to provide reference for the application of humic acid in melon cold resistance.The results showed that humic acid treatment significantly increased the root vigor，chlorophyll content and net photosynthetic rate of seedlings under low temperature stress conditions，significantly decreased the cytoplasmic membrane permeability and MDA content of seedlings under low temperature stress conditions，and increased antioxidant enzymes (SOD，POD) activity，and 300 mg?L-1 treatment reached the maximum value.In conclusion，different concentrations of humic acid treatments had significant differences in photosynthetic characteristics，growth and physiological indexes of melon seedlings under low temperature stress，and the treatment with humic acid concentration of 300 mg?L-1 had the best effect.
参考文献/References:
[1]焦自高,孙建磊,朱晓蕾,等.大拱棚厚皮甜瓜早春优质高效生产技术[J].农业知识,2021(4):18-21.[2]HUANG W，ZHANG S B，CAO K F.The different effects of chilling stress under moderate light intensity on photosystem Ⅱ compared with photosystem I and subsequent recovery in tropical tree species[J].Photosynthesis Research，2010,103(3):175-182.[3]江梦圆,杨再强,王明田,等.花期低温寡照对番茄植株生长及果实发育的影响[J].江苏农业科学,2018,46(7):125-131.[4]刘丰娇,张晓伟,李福德，等.黄瓜富氢水浸种对低温下幼苗光合碳同化及氮代谢的影响[J].园艺学报，2020，47(2):287-300.[5]韩敏,曹逼力,刘树森，等.低温胁迫下番茄嫁接苗根穗互作对叶片光合作用及氮代谢的影响[J].园艺学报，2018，45(5):897-907.[6]吴佩,崔金霞,杨志峰等.外源一氧化氮对低温下黄瓜幼苗光系统Ⅱ原初光化学反应及光合机构活性的影响[J].植物生理学报,2019(6):11.[7]张燕,方力,姚照兵.PEG对烟草幼苗耐低温胁迫能力的生理效应[J].西北农业学报,2003(1)：63-67.[8]白亚丽.日光温室果菜有机肥替代及化肥减施对其生长和土壤环境的影响[D].郑州:河南农业大学,2018.[9]裴瑞杰,王俊忠,冀建华,等.腐殖酸肥料与氮肥配施对土壤理化性质的影响[J].江苏农业科学,2018,46(19):331-334.[10]贺笑.氮肥和腐殖酸配施对藜麦生长、产量及氮肥利用率的影响[D].太原:山西师范大学，2019.[11]YAN L，FENG F，JIAN L W，et al.Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping peanut：A three-year experiment[J].Scientific Reports，2019,9(10).[12]赵兴俊,张涛.腐殖酸肥料对黄瓜苗期形态的影响[J].农业与技术,2015,35(18):1-8.[13]刘敏,李絮花,刘文博,等.腐殖酸对番茄苗期氮素代谢的影响[J].腐殖酸,2020(5):54.[14]闫军营,孙笑梅,程传凯,等.腐殖酸与氮肥配施对豫北潮土冬小麦光合特性的影响[J].江苏农业科学,2020,48(21):104-110.[15]姚艳艳.“含腐殖酸水溶肥料”在辣椒上的肥效试验[J].现代农业,2016(4):11-12.[16]王春良,李锋,刘晓丽,等.腐殖酸钠与四种农药混用对苹果黄蚜的增毒效果[J].腐殖酸,2012(2):45.[17]申逸杰.腐殖酸水溶肥料对马铃薯抗旱生理特性的影响[D].呼和浩特:内蒙古农业大学,2016.[18]邹琦.植物生理学实验指导.[M].北京:中国农业出版社,2000.[19]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003[20]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[21]王春萍,黄启中,雷开荣,等.低温下辣椒幼苗叶绿素荧光特性及其与品种耐性的关系[J].园艺学报,2015,42(9):1798-1806.[22]闫军营,孙笑梅,程传凯,等.腐殖酸与氮肥配施对豫北潮土冬小麦光合特性的影响[J].江苏农业科学,2020,48(21):104-110.[23]王红艳.嫁接对甜瓜幼苗耐低温性及光合产物运转的影响[D].沈阳:沈阳农业大学,2018.[24]刘文革,王鸣,阎志红,等.冷锻炼对不同倍性西瓜幼苗SOD、POD活性及MDA含量的影响[J].西北植物学报,2004,24(4):5.[25]赵立坤,高志奎,王梅,等.果袋微域环境对温室小型番茄果实生长及果实光系统Ⅱ活性的影响[J].北方园艺,2013(2):5-9.[26]熊炳平,雷泞菲,刘金渠,等.低温胁迫下常春藤对外源脱落酸的生理响应[J].北方园艺,2022(3):71-78.[27]张庆怡,李悦,董佳伟,等.外源ABA对低温胁迫下牛皮杜鹃气孔性状及光合性能的影响[J].北方园艺,2022(1):72-79.[28]张攀,柴华,杨梅,等.低温胁迫下黄花苜蓿根部的渗透调节和抗氧化能力变化[J].黑龙江畜牧兽医,2021(19):113-117.[29]刘倩倩,马寿宾,冯希环,等.嫁接对高温和低温胁迫下辣椒幼苗快速叶绿素荧光诱导动力学特性的影响[J].园艺学报,2016，43(5):885-896.[30]李慧,王妙媛,彭立新,等.NaCl胁迫对胡卢巴幼苗抗氧化酶活性和丙二醛含量的影响[J].华北农学报,2012,27(2)185-188.[31]白静.低温胁迫下不同抗寒性甘蓝型冬油菜生理响应及DNA甲基化研究[D].兰州:甘肃农业大学,2019.[32]徐红霞,周慧芬,李晓颖,等.低温胁迫下枇杷不同发育阶段的花果转录组比较分析[J].园艺学报,2021,48(9):1680-1694.[33]李盼华.低温胁迫对早春开花植物花器官抗寒性及花色的影响[D].郑州:河北农业大学,2011.[34]田景花.核桃低温胁迫下的生理反应及钙信使系统的适应性变化[D].郑州:河北农业大学,2008.[35]张陇艳.陆地棉对低温胁迫的响应及耐冷性鉴定[D].郑州:河北农业大学,2021.[36]刘宛,李培军,周启星,等.短期菲胁迫对大豆幼苗超氧化物歧化酶活性及丙二醛含量的影响[J].应用生态学报,2003,14(4):581-584.
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备注/Memo
第一作者简介：杨志桃（1995-），女，硕士研究生，研究方向为甜瓜栽培及生理。E-mail：1442282496@qq.com.责任作者：张彦萍（1966-），女，博士，教授，博士生导师，现主要从事设施蔬菜逆境生理及生长调控等研究工作。E-mail：18330236116@163.com.基金项目：国家西甜瓜产业技术体系资助项目（CARS-26-25）；河北省现代种业科技创新专项资助项目（21326306D）；河北省科技支撑资助项目（21327210D）；河北省农业科技成果转化资金专项资助项目（20826901D）；河北省四个一科技示范基地资助项目（2060405）；河北省蔬菜产业体系岗位专家资助项目(HBCT2018030202)。收稿日期：2022-04-08