LIU Yulian,WU Yuxia,HUANG Chengjian,et al.Changes in Carbohydrate Content of ‘Ruaner Pear’ During Ambient Temperature Storage and Freezing-thawing Period[J].Northern Horticulture,2022,(18):83-89.[doi:10.11937/bfyy.20220628]
‘软儿梨’常温贮藏和冻融过程中糖类的变化
- Title:
- Changes in Carbohydrate Content of ‘Ruaner Pear’ During Ambient Temperature Storage and Freezing-thawing Period
- Keywords:
- ‘Ruaner Pear’; room temperature; freezing-thawing; after-ripening; sugar
- 文献标志码:
- A
- 摘要:
- 以‘软儿梨’为试材,采用分光光度法和高效液相色谱等方法,研究了常温贮藏及冻融对可溶性固形物、淀粉、淀粉酶及果糖、葡萄糖、蔗糖和山梨醇等指标的影响,以期为‘软儿梨’的采后贮藏和加工提供参考依据。结果表明:常温贮藏条件下‘软儿梨’可溶性固形物含量随贮藏时间延长而呈现上升趋势,而解冻过程出现先增加后降低的趋势,并在第4天可溶性固形物含量最高,且在后熟过程中可溶性固形物含量始终高于常温后熟;2种方式后熟过程中 ‘软儿梨’淀粉含量呈明显下降趋势,淀粉酶含量均出现先上升后迅速下降的趋势,冻融过程中淀粉含量始终高于常温后熟;常温后熟过程中果糖、葡萄糖和蔗糖含量呈“上升-下降”的趋势,而山梨醇含量始终呈下降趋势,在解冻过程中果糖含量始终呈现上升趋势,但上升较为缓慢,而蔗糖含量始终呈现下降趋势,葡萄糖和山梨醇含量与常温后熟变化趋势相同。冻融后熟可明显缩短后熟时间,显著提高果实中可溶性固形物含量,但其淀粉的降解没有常温后熟充分,可溶性糖含量在2种后熟过程中变化趋势有所差异,但最终对于糖含量的影响并不明显。
- Abstract:
- Taking ‘Ruaner Pear’ as the test material,the effects of normal temperature storage and freezing-thawing on soluble solids,starch,amylase,fructose,glucose,sucrose and sorbitol were studied by spectrophotometry and high performance liquid chromatography,in order to provide reference for the post harvest storage and processing of ‘Ruaner Pear’.The results showed that the soluble solids content of ‘Ruaner Pear’ increased with storage time,and increased first and then decreased during thawing,and the highest value of soluble solids appeared on the 4th day,and the soluble solids content was always higher than that of ‘Ruaner Pear’ during post-ripening.The starch content of ‘Ruaner Pear’ showed an obvious downward trend but amylase content showed an upward trend and a rapid downward trend,and the starch content in freezing-thrawing process was always higher than normal temperature.During the normal temperature storage,fructose,glucose and sucrose showed a trend of the rise and fall,but the content of sorbitol was always a downward trend.In the process of thawing fructose presented a trend of increasing all the time,but it increased more slowly,and sucrose showed a trend of decline,glucose and sorbitol showed same trends under normal temperature.Experimental results showed that post-ripening time could be significantly shortened after freezing-thawing,and soluble solids content in fruits could be significantly increased,but starch degradation was not as sufficient as post-ripening at room temperature.The variation trend of soluble sugars in the two post-ripening processes was different,but the final effect on sugar content was not obvious.
参考文献/References:
[1]张莹,曹玉芬,田路明,等.秋子梨野生资源部分表型性状遗传多样性评价及观赏优异资源的筛选[J].中国果树,2019(6):91-94.[2]王文辉,佟伟,贾晓辉.我国冻梨生产历史,产业现状与问题分析[J].保鲜与加工,2015(6):1-6.[3]张忠,马朝玲,丁若珺,等.不同解冻方式对软儿梨果实品质与抗氧化物质含量的影响[J].食品科学,2018,39(3):236-244.[4]LIU Y,WU Y,CHE F,et al.Physical-chemical composition and quality related changes in Ruaner pear (Pyrus ussuriensis) during freezing-thawing period[J].Molecules,2019,24(14):2611.〖JP〗[5]任世霞.软儿梨中有效成分的探究及相关产品的开发[D].西宁:青海师范大学,2011.[6]赵生元,宋柯.软儿梨果酒的研制[J].酿酒科技,2009(1):87-88.[7]杨生辉,罗光宏.无糖软儿梨含片生产工艺研究[J].食品科技,2008(1):42-45.[8]马姝雯,李建萍.软儿梨汁饮料的研制[J].饮料工业,2002,5(6):21-24.[9]杨晓龙,王文辉,王志华,等.五种秋子梨果实采后品质变化及生理差异研究[J].保鲜与加工,2017,3(694):34-39.[10]潘晓倩,申琳,生吉萍.苹果采后软化过程中糖类物质代谢的研究进展[J].中国食物与营养,2011(11):29-32.[11]张永平,乔永旭,俞景权,等.园艺植物果实糖积累的研究进展[J].中国农业科学,2008,41(4):1151-1157.[12]YANG X T,SONG J,CAMPBELL-PALMER L,et al.Effect of ethylene and 1-MCP on expression of genes involved in ethylene biosynthesis and perception during ripening of apple fruit[J].Postharvest Biology Technology,2013,78:55-66.[13]于年文,李俊才,王家珍,等.不同时期采收的南果梨后熟过程中糖分的变化规律[J].中国农学通报,2011,27(20):123-126.[14]YU N W,LI J C,WANG J ZH,et al.The Changes about sugar components of Nanguo Pear of different harvest times during after-ripening[J].Chinese Agricultural Science Bulletin,2011,27(20):123-126.[15]BEN-ARIE R,KISLEV N.Ultrastructural changes in the cell wallsof ripening apple and pear fruit[J].Plant Physiology,1979,64:197-202.[16]YOSHIOKA H,AOBA K,KASHIMURA Y.Molecular weight and degree of methoxylation in cell wall polyuronide during softening inpear and apple fruit[J].Journal of the American Society for Horticultural Science,1992,117(4):600-606.[17]周宏伟,吴耕西.长把梨贮藏中多聚半乳糖醛酸酶与果胶甲酯酶的作用[J].山东农业大学学报,1992,23(1):67-70.[18]王宪泽.生物化学实验技术原理和方法[M].北京:中国农业大学出版社,2002.[19]岳虹,赵贞,刘丽君,等.高效液相色谱法测定发酵乳饮料中的果糖、葡萄糖、蔗糖、麦芽糖及乳酸含量[J].乳业科学与技术,2017,40(2):23-26.[20]王绍美,赵讲芬.茶多酚对采后猕猴桃果实生理生化变化的影响[J].果树科学,2000,17(4):273-276.[21]赵纪伟.不同安梨株系果实贮藏过程中品质变化研究[D].保定:河北农业大学,2011.[22]陈俊伟,张上隆,张良诚.果实中糖的运输、代谢与积累及其调控[J].植物生理与分子生物学学报,2004(1):1-10.[23]DENG L L,PAN X Q,CHEN L,et al.Effects of preharvestnitric oxide treatment on ethylene biosynthesis and soluble sugars metabolism in‘Golden Delicious’apples[J].Posthar-vest Biology Technology,2013,84:9-15.[24]于年文,李俊才,王家珍,等.南果梨果实糖积累过程中相关酶的作用[J].西南农业学报,2011(3):919-923.[25]王永章,张大鹏.发育过程中苹果果实的β-淀粉酶:活性、数量变化和亚细胞定位[J].中国科学(C辑),2002,32(3):201-210.[26]徐曈晖,李洋.冻融方法对果蔬品质维持的研究进展[J].食品工业,2021,42(5):240-252.[27]何娇.冷冻对果蔬制品的影响[J].农产品加工,2011(9):11.[28]齐秀东,魏建梅,赵美微,等.‘京白梨’果实后熟软化与糖、淀粉代谢及其基因表达的关系[J].中国农业科学,2015,48(13):2591-2599.[29]RUAN Y L.Sucrose metabolism:gateway to diverse carbon use and sugar signaling[J].Annual Review of Plant Biology,2014,65(1):33-67.[30]苏静,祝令成,刘茜,等.果实糖代谢与含量调控的研究进展[J].果树学报,2022(2):266-279.
相似文献/References:
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备注/Memo
第一作者简介:刘玉莲(1975-),女,博士,副教授,现主要从事果树遗传育种和品质改良等研究工作。E-mail:yulianliu@126.com.基金项目:达州市重点研发计划资助项目(21ZDYF0020);甘肃农业大学学科建设基金资助项目(GAU-XKJS-2018-227)。收稿日期:2022-02-23