CHEN Jingtian,YANG Yunshu,YANG Wenyi,et al.Breeding of Excellent Material Salt Tolerant Mutants of Stevia rebaudiana by 60Co-γ Ray Irradiation[J].Northern Horticulture,2022,(09):100-108.[doi:10.11937/bfyy.20213831]
60Co-γ射线辐射甜叶菊诱变选育优质耐盐突变体
- Title:
- Breeding of Excellent Material Salt Tolerant Mutants of Stevia rebaudiana by 60Co-γ Ray Irradiation
- 文献标志码:
- A
- 摘要:
- 以甜叶菊叶片愈伤组织为突变体材料，创制并筛选耐盐突变体材料。采用 60Co-γ射线辐射对甜叶菊叶片愈伤组织进行诱变的方法，利用高盐培养基进行初步筛选，然后对初筛后的材料进行水培盐胁迫处理复筛（100 mmol?L-1NaCl、60 mmol?L-1Na2SO4、70 mmol?L-1NaHCO3营养液、处理20 d），研究突变体的株高、茎粗、节间长、叶鲜质量、茎鲜质量、叶干质量、茎干质量、叶长、叶宽等主要农艺性状，分析各类型糖苷含量，以期为甜叶菊优良品种选育提供参考依据。结果表明：经过辐射诱变后，成功得到13株再生苗，初步耐盐筛选共得到9份耐盐材料，复筛后得到7份耐盐突变体材料。1~3号为耐NaCl植株，4~7号为耐Na2SO4植株，未得到耐NaHCO3突变体植株。7份耐盐突变体材料农艺性状和糖苷含量均出现了不同程度的变异，最终得到1份综合性状优良的耐NaCl突变体材料。通过对突变体的创制和筛选，共得到7份耐盐新材料，1份优良材料。
- Abstract:
- Taking Stevia rebaudiana leaf callus as mutant materials,a salt-tolerant mutant material was created and screened.The callus of Stevia rebaudiana leaves was induced by 60Co-γ ray radiation,and the high-salt medium was used for preliminary screening.Then 100 mmol?L-1 NaCl,60 mmol?L-1 Na2SO4 and 70 mmol?L-1 NaHCO3 nutrient solution were used to treat the material after the initial screening for 20 days under water culture salt stress.The plant height,stem diameter,internode length,leaf fresh weight,stem fresh weight,leaf dry weight,stem dry weight,leaf length,leaf width and other major agronomic traits were measured,and the content of glycosides of various types was analyzed,in order to provide reference for the breeding of exceltent varieties of Stevia rebaudiana Bertoni.The results showed that after radiation mutagenesis,13 regenerated seedlings were successfully obtained,9 salt-tolerant materials were obtained through preliminary salt-tolerant screening,and 7 salt-tolerant mutant materials were obtained after re-screening.No.1-3 were NaCl-tolerant plant,No.4-7 were Na2SO4-tolerant plant,and no NaHCO3-tolerant mutant plant had been obtained.The agronomic characters and glucoside contents of 7 Rinds salt-tolerant mutant materials all showed varying degrees of variation,and finally a NaCl-tolerant mutant material with excellent comprehensive properties was obtained.Through the creation and screening of mutants,7 new salt tolerant materials and 1 excellent material were obtained.
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备注/Memo
第一作者简介：陈竞天(1995-)，男，硕士研究生，研究方向为药用植物资源评价与利用。E-mail:605722405@qq.com.责任作者：吴卫（1970-），女，博士，教授，现主要从事药用植物资源评价与利用等研究工作。E-mail:ewuwei@sicau.edu.cn.基金项目：国家自然科学基金资助项目（31671757）；四川省中医药重点学科建设资助项目（2021-16-4）。收稿日期：2021-09-14