WU Ting,GE Hong,YANG Shuhua,et al.Colchicine Induces Polyploidy in the Seeds of Phalaenopsis zhejiangensis[J].Northern Horticulture,2022,(02):71-78.[doi:10.11937/bfyy.20213229]
秋水仙素诱导象鼻兰种子产生多倍体
- Title:
- Colchicine Induces Polyploidy in the Seeds of Phalaenopsis zhejiangensis
- Keywords:
- Phalaenopsis zhejiangensis; seeds; colchicine; polyploid
- 文献标志码:
- A
- 摘要:
- 以象鼻兰种子为试验材料，采用浸泡法，研究了秋水仙素处理对蝴蝶兰种子染色体加倍的影响，以期获得诱导多倍体的适宜条件。结果表明：秋水仙素浸泡法诱导种子染色体加倍时，种子在液体培养基中培养5 d，再采用0.20%秋水仙素处理1 d的处理效果最好，萌发率达到6.41%，高于1.96%的自然萌发率，四倍体、嵌合体诱导率分别达到了27.75%、34.84%；四倍体植株与二倍体植株相比，形态与气孔特征均发生了明显变化，形态特征表现为，植株粗壮、根系发达且粗壮、植株生长较快等；气孔特征表现为单位面积气孔数减少，气孔增大，长宽分别增长25.19%和26.19%。该研究得到了秋水仙素浸泡象鼻兰种子诱导染色体加倍的适宜条件且提高了象鼻兰种子的萌发率，获得了四倍体植株和嵌合体植株，为把象鼻兰优异基因高效导入栽培品种奠定了基础，为蝴蝶兰体细胞无性系变异新品种的培育提供了参考依据。
- Abstract:
- Phalaenopsis zhejiangensis seeds were used as materials to explore the effect of colchicine treatment on the chromosome doubling,and was studied by immersion method to explore the suitable conditions for obtaining polyploidy,in order to obtain suitable conditions for inducing polyploidy.The results showed that the survival rate reached 6.41%,and tetraploid,and chimera rates induction were 27.75% and 34.84%,respectively,when the seeds were cultured in liquid medium for 5 days,and then 0.20% colchicine treatment for 1 day.Compared with diploid plants,the morphological and stomatal characteristics of tetraploid plants changed significantly.The plant was stronger,the root system was developed and robust,and the plant grew faster.The number of stomata per unit area decreased,but the length and width of stomata increased by 25.19% and 26.19%,respectively.In this study,suitable conditions for colchicine to induce chromosome doubling were obtained by soaking Phalaenopsis zhejiangensis seeds.Tetraploid plants and chimera plants were obtained,which laid the foundation for the efficient introduction of excellent genes from Phalaenopsis zhejiangensis into cultivated varieties.
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备注/Memo
第一作者简介：吴婷（1997-），女，硕士研究生，研究方向为植物遗传资源育种。E-mail：18811023178@163.com.责任作者：贾瑞冬（1980-），女，博士，副研究员，现主要从事花卉种植资源与育种等研究工作。E-mail：jiaruidong@caas.cn.基金项目：国家重点研发计划资助项目（2019YFD1001003）；农业部园艺作物生物学与种质创制重点实验室资助项目。收稿日期：2021-08-06