ZHANG Qingyi,LI Yue,DONG Jiawei,et al.Effects of Exogenous ABA on Stomatal Characters and Photosynthetic Characteristics of Rhododendron chrysanthum Pall. Under Low Temperature Stress[J].Northern Horticulture,2022,(01):72-79.[doi:10.11937/bfyy.20213003]
外源ABA对低温胁迫下牛皮杜鹃气孔性状及光合性能的影响
- Title:
- Effects of Exogenous ABA on Stomatal Characters and Photosynthetic Characteristics of Rhododendron chrysanthum Pall. Under Low Temperature Stress
- 文献标志码:
- A
- 摘要:
- 以牛皮杜鹃（Rhododendron chrysanthum Pall.）为试材,采用脱落酸（ABA）预处理4 d,常温和低温胁迫下培养2 d后，分别对其进行叶绿素荧光含量与气孔大小测定，研究了外源ABA对低温胁迫下牛皮杜鹃PSⅡ光合特性与气孔性状的影响，以期为探究其抗寒性机制提供参考依据。结果表明：牛皮杜鹃幼苗的正常生长由于低温胁迫，受到显著抑制，导致PSⅡ最大光化学效率（Fv/Fm）、光化学淬灭系数（qP）以及非光化学淬灭系数（NPQ）、潜在活性（Fv/Fo）显著下降（P<0.05）。外源ABA能够显著缓解低温胁迫对牛皮杜鹃叶片光合能力的影响,优化气体交换过程，对气孔长度、宽度、长宽比影响显著（P<0.05），提高PSⅡ反应中心活性，提高光能利用率，促进物质积累，提高牛皮杜鹃对低温胁迫的耐受性。
- Abstract:
- Rhododendron chrysanthum Pall. was used as the test materials,after 4 days of pretreatment with ABA,and 2 days of culture under normal temperature and low temperature stress,the chlorophyll fluorescence content and stomata size were measured respectively. The effects of PSⅡ photosynthetic characteristics and stomatal traits of Rhododendron chrysanthum Pall.on exogenous ABA under low temperature stress were studied,in order to provide a reference basis for exploring the mechanism of cold resistance.The results showed that the normal growth of Rhododendron chrysanthum Pall. seedlings were significantly inhibited by low temperature stress,the maximum photochemical efficiency (Fv/Fm),photochemical quenching coefficient (qP),nonphotochemical quenching (NPQ) and potential activity (Fv /Fo) of PSⅡ decreased significantly (P<0.05). Under low temperature stress,exogenous ABA could significantly alleviate the effects on photosynthetic capacity of Rhododendron chrysanthum Pall. leaves,optimize the gas exchange
参考文献/References:
[1]ZHOU X F,CHEN S,WU H,et al.Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall[J].BioMed Central,2017,12(1):10.[2]郭贵华,刘海艳,李刚华,等.ABA缓解水稻孕穗期干旱胁迫生理特性的分析[J].中国农业科学,2014,47(22):4380-4391.[3]刘璐,刘芸伯,佟佳欣,等.低温胁迫下牛皮杜鹃MAPK级联参与ABA 信号转导的基因表达分析[J].江苏农业科学,2020,48(17):59-65.[4]李明军.植物与植物生理[M].重庆:重庆大学出版社,2015.[5]任昱,魏春光,郭小宇.6种荒漠植物叶片气孔性状比较分析[J].内蒙古农业大学学报(自然科学版),2021,42(2):21-26.[6]LIM C W,LUAN S,LE E，et al.A prominent role for RCAR3-mediated ABA signaling in response to Pseudomonas syringae pv.tomato DC3000 infection in Arabidopsis.[J].Plant & Cell Physiology,2014,55(10):1691-703.[7]勾畅.长白山牛皮杜鹃抗逆机理及耐寒microRNA393的初步研究[D].四平：吉林师范大学,2015.[8]朱健康,倪建平.植物非生物胁迫信号转导及应答[J].中国稻米,2016,22(6):52-60.[9]WANG P J,JIN S,CHEN X J,et al.Chromatin accessibility and translational landscapes of tea plants under chilling stress.[J].Hortic Res,2021,8(1):96.[10]安爽,高玉迪,麦迪努尔·玉苏普,等.外源脱落酸抑制蓝莓早花及相关基因表达特性研究[J].果树学报,2021,38(3):325-334.[11]郑淑霞,上官周平.近一世纪黄土高原区植物气孔密度变化规律[J].生态学报,2004(11):2457-2464.[12]许耀照,张芬琴,陈修斌,等.低温胁迫对彩椒幼苗生长指标及光合特性的影响[J].山西农业大学学报(自然科学版),2019,39(1):68-72.[13]项洪涛,李琬,郑殿峰,等.外源ABA对低温胁迫下小豆幼苗生理及产量的影响[J].干旱地区农业研究,2020,38(6):52-60.[14]张爱冬,张圣美,朱宗文,等.不同设施自然低温对丝瓜幼苗生长和生理特性的影响[J].江西农业学报,2019,31(10):34-38.[15]杨碧云,钟凤林,林义章.干旱对紫色小白菜光合特性及营养品质的影响[J].西北植物学报,2018,38(5):912-921.[16]段娜,贾玉奎,徐军,等.植物内源激素研究进展[J].中国农学通报,2015,31(2):159-165.[17]XIANG H，WANG T,ZHENG D,et al.ABA pretreatment enhances the chilling tolerance of a chilling-sensitive rice cultivar[J].Brazilian Journal of Botany,2017,40(4):853-860.[18]岑海燕,姚洁妮,翁海勇,等.叶绿素荧光技术在植物表型分析的研究进展[J].光谱学与光谱分析,2018,38(12):3773-3779.[19]DOU L R,HE K K,PENG J L,et al.The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA.[J].Nature communications,2021,12(1):2181.[20]项洪涛,齐德强,李琬,等.低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响[J].草业学报,2019,28(4):81-94.[21]张浩,吴子龙,付伟,等.外源脱落酸对NaCl盐胁迫下玉米幼苗生长、气孔特征及光合性能的影响[J].生态学杂志,2021,18(6):2005-2015.
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备注/Memo
第一作者简介：张庆怡（1998-），女，硕士研究生，研究方向为遗传学。E-mail：913819514@qq.com.责任作者：周晓馥（1964-），女，博士，教授，博士生导师，现主要从事植物分子生物学等研究工作。E-mail:zhouxiaofu@ jlnu.edu.cn.基金项目：吉林省科技发展计划资助项目（20190301059NY）；中央指导地方科技发展资金吉林省基础研究专项资助项目（202002016JC）。收稿日期：2021-07-16