GAO Zhu,CHEN Lu,ZHANG Xiaoli,et al.Effects of Interplanting Lupin on Dynamic Changes of Antioxidant Enzymes Activity in Kiwifruit[J].Northern Horticulture,2021,(22):38-46.[doi:10.11937/bfyy.20211438]
羽扇豆套种对猕猴桃抗氧化酶活性动态变化的影响
- Title:
- Effects of Interplanting Lupin on Dynamic Changes of Antioxidant Enzymes Activity in Kiwifruit
- Keywords:
- lupin; kiwifruit; interplanting; antioxidant enzymes
- 文献标志码:
- A
- 摘要:
- 以五年生早熟“红阳”、晚熟“金艳”和“金魁”猕猴桃品种为试材，采用套种与不套种羽扇豆的比较方法，研究了羽扇豆套种后对不同时期3种猕猴桃叶片和果实中5种抗氧化酶活性（SOD、CAT、POD、GR和APX）动态变化的影响，以期为羽扇豆作为绿肥作物栽培对促进夏季猕猴桃植株生长和果实发育影响的研究提供参考依据。结果表明：3种猕猴桃叶片和果实5种抗氧化酶活性变化趋势为先升高后降低。“红阳”叶片谷胱甘肽还原酶（GR）活性、“金艳”叶片GR和抗坏血酸过氧化物酶（APX）活性、“金魁”叶片过氧化氢酶（CAT）、过氧化物酶（POD）和APX活性平均值高于果实。套种羽扇豆后，“红阳”叶片POD活性峰值与“金艳”和“金魁”叶片GR活性峰值、“金艳”果实超氧化物歧化酶（SOD）和GR活性峰值与“金魁”果实CAT和GR活性峰值比对照提前15~30 d到达；“红阳”叶片GR活性和果实CAT活性，以及“金艳”和“金魁”叶片与果实的POD活性比对照活性提高最大。可见，套种羽扇豆后，不仅可以影响3种猕猴桃叶片和果实抗氧化酶活性峰值到达的时间，而且显著提高了不同生长时期的抗氧化酶平均活性；其中对3种猕猴桃GR活性影响最大，POD和CAT活性影响次之，APX和SOD活性影响较小。综上所述，“金艳”果实具有较强的自我保护系统，“金魁”果实具有较强的耐热性。
- Abstract:
- Five-year-old kiwifruit early-maturing cultivar ‘Hongyang’ and late-maturing cultivar ‘Jinyan’ and ‘Jinkui’ were used as experimental materials,the comparison method of intercropping and non-intercropping lupin were used to explore the effects on the activities of five antioxidant enzymes (SOD,CAT,POD,GR and APX) in leaves and fruits of three kiwifruit cultivars at different growth stages,in order to provide a reference for the interplanting lupin as green fertilizer to promote kiwifruit plant growth and fruit development in summer.The results showed that the activities of five antioxidant enzymes in leaves and fruits of three kiwifruit cultivars increased first and then decreased.The activities of GR in ‘Hongyang’ leaves，GR and APX in ‘Jinyan’ leaves，CAT，POD and APX in ‘Jinkui’ leaves were higher than those in fruits.After interplanting lupin，the peak values of POD activities in ‘Hongyang’ and GR activities in ‘Jinyan’ and ‘Jinkui’ leaves，as well as SOD and GR activities in ‘Jinyan’ and CAT and GR activities in ‘Jinkui’ fruit were reached 15-30 days earlier than the control.In addition，the GR activities in leaves and CAT activities in fruits of ‘Hongyang’，POD activities in leaves and fruits of ‘Jinyan’ and ‘Jinkui’ increased the most than that of the control.These showed that the peak time of antioxidant enzyme activities in the leaves and fruits of three kiwifruit cultivars were not only affected，but the average antioxidant enzyme activities in different growth stages were significantly improved.Among them，GR activities had the greatest influence，POD and CAT activities had the second，and APX and SOD activities had little influence in three kiwifruit cultivars.The comprehensive study showed that ‘Jinyan’ fruit had a strong self-protection system，and ‘Jinkui’ fruit had a strong heat resistance.
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备注/Memo
第一作者简介：高柱（1981-），男，博士，副研究员，现主要从事抗逆性植物筛选及高值农业等研究工作。E-mail:jxaugz2008@126.com.责任作者：王小玲（1979-），女，博士，研究员，现主要从事园艺植物高值利用等研究工作。E-mail：wangxiaoling1979@126.com.基金项目：江西省科学院高端科技人才专项基金资助项目（2020GDRC1）；江西省重点研发计划资助项目（20181BBF60009，20192BBHL80002）。收稿日期：2021-04-02