GE Lili,LI Wei,XUE Jungang,et al.Genetic Diversity Analysis of AFLP With Different Altitude Lonicera caerulea in Changbai Mountain[J].Northern Horticulture,2021,(18):48-53.[doi:10.11937/bfyy.20210799]
长白山不同海拔蓝靛果忍冬AFLP遗传多样性分析
- Title:
- Genetic Diversity Analysis of AFLP With Different Altitude Lonicera caerulea in Changbai Mountain
- Keywords:
- Lonicera caerulea; altitude; AFLP; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以吉林省长白山地区4个海拔梯度(600、1000、1 400 m和1 800 m)的野生蓝靛果忍冬为试材,采用AFLP分子标记技术,研究了不同海拔梯度野生蓝靛果忍冬间的遗传多样性,以期为蓝靛果忍冬野生资源的保护与利用和优质种质资源的选育提供参考依据。结果表明:野生种群间Nei基因多样性指数和Shannon信息指数的均值分别为0.467 5和0.660 2。总基因多样度为0.462 0,基因分化系数为0.006 5,说明总体上不同海拔种群间遗传多样性较高。各种群遗传多样性从高到低依次为1 400、1 000、680 m和1 800 m。基于遗传距离通过非加权组平均法(UPGMA)将4个海拔种群分为2组,其中低海拔600 m和1 000 m一组;高海拔1 400 m和1 800 m一组。
- Abstract:
- Taking the wild Lonicera caerulea of four altitude gradient that including 600 m,1 000 m,1 400 m and 1 800 m in Changbai Mountain of Jilin Province.AFLP molecular marker technology was used,the genetic diversity of wild blue honeysuckle between different altitude was studied,in order to provide reference for the conservation and utilization of wild resources of Lonicera caerulea and the breeding of high-quality germplasm resources.The results showed that the averages of genetic diversity index was 0.467 5,and the averages of Shannon information index was 0.660 2.The total gene diversity was 0.462 0 and the gene differentiation coefficient was 0.006 5,that suggests genetic diversity was higher among populations at different altitudes.The genetic diversity of each population from high to low was 1 400 m,1 000 m,680 m and 1 800 m.Based on genetic distance,the four altitudes were divided into two groups by unweighted group mean method (UPGMA),among which one was the low altitudes 600 m and 1 000 m and another was high altitude 1 400 m and 1 800 m.
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备注/Memo
第一作者简介:葛丽丽(1978-),女,硕士,实验师,现主要从事森林培育理论与技术等研究工作。E-mail:27208964@qq.com.责任作者:其其格(1970-),女,硕士,教授,现主要从事风景园林规划设计及园林植物应用等研究工作。E-mail:qqgsun@sina.com.基金项目:国家自然科学基金资助项目(31770667);中央财政林业科技推广示范资助项目(JLT2019-04)。收稿日期:2021-02-24