SHANG Xiaojing,LUO Nixing,ZHANG Fumei,et al.Identification of Alternaria tenuissima Pathogen of Raspberry Leaf Blotch and Its Biological Characteristics[J].Northern Horticulture,2021,(16):24-32.[doi:10.11937/bfyy.20210163]
树莓极细链格孢菌叶斑病病原菌鉴定及其生物学特性
- Title:
- Identification of Alternaria tenuissima Pathogen of Raspberry Leaf Blotch and Its Biological Characteristics
- Keywords:
- raspberry; leaf blotch; Alternaria tenuissima; identification of pathogens; biological characteristics
- 文献标志码:
- A
- 摘要:
- 从贵州省贵阳市树莓园的受害树莓叶片分离出致病菌S1，经致病性测定，形态学和分子生物学rDNA-ITS 序列相似性分析，鉴定病原菌S1为极细链格孢菌(Alternaria tenuissima)。采用控制单一变量法研究树莓极细链格孢菌叶斑病病原菌生物学特性，以期为由该致病菌所引起的树莓病害防治提供参考依据。结果表明：病原菌S1于25 ℃的恒温环境中生长最好，15 ℃时产孢量最高；最适生长pH为11，最适产孢pH为7；在24 h持续黑暗的环境中生长速度最快，产孢量最高的光照条件为半光照；碳源分别为淀粉和乳糖时生长最好和产孢量最高；以牛肉膏为氮源时生长最好，添加酵母粉时产孢量最多；在树莓煎叶培养基中生长最快，产孢量最多的培养基是PDA。
- Abstract:
- The pathogen S1 was isolated from the damaged raspberry leaves in the raspberry garden of Guiyang,Guizhou Province.After pathogenicity determination,morphology and molecular biology rDNA-ITS sequence similarity analysis,the pathogen S1 was identified as Alternaria tenuissima.The biological characteristics of leafy plaque pathogens were studied by using a single variable method of control,in order to provide a reference for the prevention and treatment of raspberry diseases caused by pathogenic bacteria.The results showed that the ideal temperature conditions for the pathogen S1 mycelial growth and sporulation were in the range of 25 ℃ and 15 ℃.The pathogen S1 was able to grow in a pH 11 and the optimal pH for sporulation and spore germination was 7.0.Strains grew fastest in continuous darkness,and the spore production was the highest in half light.The best carbon sources of starch and lactose was propitious to sporulation.The best nitrogen sources were beef extract for mycelial growth,and that for spore germination and sporulation was leavening agents.The highest spore production was raspberry leaf culture medium and PDA medium was the optimum media for spore production.
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备注/Memo
第一作者简介：尚晓静（1997-），女，硕士研究生，研究方向为林木病理学。E-mail:shangxiaojingcg@163.com.责任作者：侯瑞（1988-），女，博士，副教授，现主要从事林木病理等研究工作。E-mail:jiayouhourui123@163.com.基金项目：贵州省科技计划资助项目（黔科合支撑[2017]2567）；黔科合平台人才资助项目（[2018]5781）；国家自然科学基金资助项目（31801688）。收稿日期：2020-01-13