HAO Dazhi,CHEN Jie,LIU Peng,et al.Effects of Seaweed Residue Combined With Microbial Agent on Cucumber Seedling Growth[J].Northern Horticulture,2021,(19):1-7.[doi:10.11937/bfyy.20210142]
海藻渣复配微生物菌剂对黄瓜幼苗生长的影响
- Title:
- Effects of Seaweed Residue Combined With Microbial Agent on Cucumber Seedling Growth
- 文献标志码:
- A
- 摘要:
- 以“申青一号”黄瓜为试材,通过测定黄瓜幼苗的出苗率、株高、根长、根部鲜质量和全株鲜质量,研究了海藻渣、棘孢木霉菌(Trichoderma asperellum)GDFS1009菌剂及解淀粉芽孢杆菌(Bacillus amyloliquefaciens)FS1841菌剂拌土处理对黄瓜幼苗生长的影响,以期筛选出海藻渣与微生物菌剂复配使用的最佳浓度,达到协同增效的目的。结果表明:1%(w/w)的海藻渣、2.0×108 cfu·g-1芽孢杆菌菌剂、3.0×105 cfu·g-1木霉菌剂对黄瓜幼苗生长有显著促生作用。1%(w/w)海藻渣与2.0×108 cfu·g-1芽孢杆菌菌剂及3.0×105 cfu·g-1木霉菌剂复配后与基质混合,可使黄瓜幼苗的根长、根质量、全株鲜质量、叶面积分别比对照提高67.25%、241.67%、136.44%、99.99%,且显著优于单独使用。
- Abstract:
- Taking ‘Shenqing No.1’ cucumber as test material,the seedling emergence rate,plant height,root length,fresh weight of root,and fresh weight of the whole plant were measured and compared respectively.The effects of seaweed residue,Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens FS1841 mixed with soil on the growth of cucumber seedlings were studied,in order to screen out the best concentration of seaweed residue and microbial inoculum,and achieve the goal of synergy.The results showed that 1%(w/w) seaweed residue,2.0×108 cfu·g-1Bacillus agent and 3.0×105 cfu·g-1Trichoderma agent could significantly promote the growth of cucumber seedlings.1%(w/w) seaweed residue,2.0×108 cfu·g-1Bacillus agent,and 3.0×105 cfu·g-1Trichoderma agent were mixed with the substrate,which could increase the root length,root weight,fresh weight of the whole plant and leaf area of cucumber seedlings by 67.25%,241.67%,136.44% and 99.99% respectively compared with the control.
参考文献/References:
[1]MEYER,S L F,ROBERTS,D P.Combinations of biocontrol agents for managment of plant-parasitic nematodes and soilborne plant-pathogenic fungi[J].Journal of Nematology,2002,34(4):1-8.[2]杨定祥.木霉菌TH-T20与解淀粉芽孢杆菌SH-27混合防治辣椒疫病初步研究[D].湛江:广东海洋大学,2018.[3]WU Q,SUN R Y,NI M.Identification of a novel fungus,Trichoderma asperellum GDFS1009,and comprehensive evaluation of its biocontrol efficacy[J].PLoS One,2017,12(6):e0179957.[4]朱梦霞,高颖,陈寅瑞,等.利用海藻渣栽培食用菌的研究[J].现代食品,2019(3):72-75,86.[5]江杰.海带残渣有机肥的制备及其效果评价[D].南京:南京农业大学,2016.[6]沈虹,王磊,苗艳,等.海藻渣对菠菜生长和品质的影响[J].江苏农业科学,2016,44(10):196-200.[7]方金,王斌,郭世荣,等.黄瓜育苗海藻渣基质配方研究[J].沈阳农业大学学报,2013,44(5):622-627.[8]黄振瑞,陈迪文,李集勤,等.海藻渣与镁配施对红壤理化性质和烟草生长的影响[J].热带作物学报,2020,41(9):1797-1802.[9]苏东海.复合菌发酵海藻渣制备海藻饲料的工艺研究[D].青岛:青岛大学,2017.[10]周莹,袁孟娟,韩军,等.丹参根腐病生防芽孢杆菌2-1海藻菌剂的研制[J].生物技术通报,2015,31(1):167-172.[11]陈伯清,屈海泳,刘连妹.木霉菌在农业上的应用研究进展[C].金华:首届长三角园艺论坛,2007.[12]HARAGUCHI,HIROYUKI H,YASUYUKI H A,et al.Effect of gliotoxin on growth and branched-chain amino acid biosynthesis in plants[J].Phytochemistry (Oxford),1996,42(3):645-648.
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备注/Memo
第一作者简介:郝大志(1997-),男,硕士研究生,研究方向为微生物农药创制。E-mail:574642689@qq.com.责任作者:陈捷(1959-),男,博士,教授,博士生导师,现主要从事植物病害生物防治等研究工作。E-mail:jiechen59@sjtu.edu.cn.基金项目:山东省重点研发计划(重大科技创新工程)资助项目(2019JZZY020707);上海市科委重点研究计划重点资助项目(18391902400)。收稿日期:2021-01-12