JING Chenjuan,CHEN Xuefeng,WANG Duan,et al.Analysis of Leaf Structure Differences and Relationships Between the Anatomical Structure and Drought Resistances of Three Plums Cultivars[J].Northern Horticulture,2021,(15):27-34.[doi:10.11937/bfyy.20205324]
三个李子品种叶片结构差异及其抗旱性分析
- Title:
- Analysis of Leaf Structure Differences and Relationships Between the Anatomical Structure and Drought Resistances of Three Plums Cultivars
- Keywords:
- plum; leaf; spongy tissue; drought resistances
- 文献标志码:
- A
- 摘要:
- 以‘龙园蜜李’‘黑宝石’‘大石早生’3个李子品种叶片为试材，利用石蜡切片和扫描电镜观察老、嫩叶叶片形态和解剖结构，采用隶属函数方法分析比较不同品种的抗旱性，以期为李子种质资源评价方法中叶片的形态解剖学鉴定提供了丰富数据基础，同时为李子抗旱性品种选育提供了参考依据。结果表明:‘大石早生’的叶片最大，叶面积为（52.93±3.95）cm2，叶形偏圆，叶宽比为1.73±0.14，‘黑宝石’叶质量最大，为（0.66±0.05）g，叶片最厚，厚度为（3.16±0.56）mm·（10叶）-1，叶形偏长，叶宽比2.50±0.13。叶片厚度和上表皮宽度可作为区分3个李子品种各自的嫩、老叶的指标，其中嫩叶厚度及其上表皮细胞宽度较小，老叶较大。3个李子品种嫩叶的上表皮细胞宽度及叶片组织结构紧密度（CTR）均表现为‘黑宝石’＜‘龙园蜜李’＜‘大石早生’，且差异显著，因此可据此区分3个品种。采用隶属函数法对叶片结构紧密度、栅海组织、气孔大小和密度等进行综合分析，发现同一李子品种的老叶抗旱性更强，其中‘大石早生’抗旱性最强（嫩叶0.580 3；老叶0.850 8），‘龙园蜜李’的抗旱性最弱（嫩叶0.324 5；老叶0.379 7）。
- Abstract:
- Three plum cultivars of ‘Longyuanmili’‘Heibaoshi’ ‘Dashizaosheng’ were used as test materials.The leaf morphology and anatomical structure of old and young leaves were observed by paraffin section and scanning electron microscope,and the drought resistance of different varieties was compared by membership function method in this experiment.In order to provide a rich data base for morphological and anatomical identification for the evaluation method of plum germplasm resources,and also provided a reference for drought-resistant seed selection of plum.The results showed that the leaf area of ‘Longyuanmili’ was the largest,(52.93±3.95)cm2,its leaf shape was slightly round (ratio of length and width was 1.73±0.14).The leaf thickness and weight of ‘Heibaoshi’ was the maximum,they were (3.16±0.56)mm·(10leaves)-1 and (0.66±0.05)g,respectively,its leaf shape was slightly long (ratio of length and width was 2.50±0.13).The leaf thickness and width of the upper epidermis could be distinguished between young and old leaves of the three plum cultivars,the leaf thickness and the width of the upper epidermis of young leaves were the minimum,while the index of old leaves were maximum.The upper epidermal cell width and leaf tissue structure tightness (CTR) of young leaves was ‘Heibaoshi’<‘Longyuanmili’<‘Dashizaosheng’.The membership function was used to make comprehensive analysis of the epidermal cell structure,palisade tissue,stomatal size and density of the plum leaf tissue,the result was that the cultivar of the strongest drought resistance was ‘Dashizaosheng’ (young leaves was 0.580 3;old leaves was 0.850 8),and the weakest plum cultivar was ‘Longyuanmili’ (young leaves was 0.324 5;old leaves was 0.379 7).
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备注/Memo
第一作者简介：景晨娟（1986-），女，博士，副研究员，现主要从事李杏果树栽培与育种等研究工作。E-mail:jingchenjuan@163.com.责任作者：武晓红（1981-），女，硕士，副研究员，现主要从事李杏果树育种及栽培管理等研究工作。E-mail：40502434@qq.com.基金项目：河北省农林科学院青年基金资助项目(2018100105)；河北省科技计划资助项目(16226313D-2)；河北省农林科学院创新工程资助项目(C19C0701-03)。收稿日期：2020-12-14