CAO Dongmei,JIA Minlong,DUAN Jiuju,et al.Effects of Exogenous Methyl Jasmonate on Photosynthetic Characteristics of Hemerocallis Under Drought Stress[J].Northern Horticulture,2021,(13):78-84.[doi:10.11937/bfyy.20205016]
外源茉莉酸甲酯对干旱胁迫下萱草光合生理的缓解效应
- Title:
- Effects of Exogenous Methyl Jasmonate on Photosynthetic Characteristics of Hemerocallis Under Drought Stress
- Keywords:
- PEG-simulated drought stress; Hemerocallis; methyl jasmonate; photosynthetic parameters; chlorophyll fluorescence parameters
- 文献标志码:
- A
- 摘要:
- 以萱草“红运”为试材，采用PEG模拟干旱胁迫的方法，研究了干旱胁迫对萱草光合生理的影响以及外源MeJA对干旱胁迫下萱草光合生理的缓解效应，以期为提高外源茉莉酸甲酯（MeJA）在萱草园林绿化抗旱栽培中的合理利用提供参考依据。结果表明：干旱胁迫显著降低了萱草叶片光合色素含量及气孔导度（Gs）、蒸腾速率（Tr）和胞间CO2浓度（Ci），提高了 PSⅡ初始荧光（Fo）和非光化学淬灭系数（NPQ），而最大荧光（Fm）、PSⅡ潜在活性（Fv/Fo）、最大光化学效率（Fv/Fm）、实际光化学效率（ΦPSⅡ）、光化学淬灭系数（qP）等受到显著抑制，最终表现为光合速率（Pn）降低。干旱胁迫下外源 MeJA 显著增加了光合色素的含量，在一定程度上可通过减少叶绿素的分解和提高叶片光合机构的稳定性来减轻干旱胁迫对萱草的伤害，且叶片Gs、Ci、Pn、Fm、Fv/Fo、Fv/Fm、ΦPSⅡ、qP均比干旱胁迫下有所增大，Fo和NPQ降低，而茉莉酸代谢抑制剂（SHAM）处理逆转了干旱胁迫下MeJA对萱草叶片光合特性的影响，表明在干旱胁迫下，外施MeJA可以减轻干旱胁迫下光合系统发生的光抑制现象，缓解干旱胁迫对萱草叶片光合机构的损伤以及 PSⅡ反应中心的伤害，改善电子传递速率和光能转换效率，从而增强叶片的气孔导度和光合能力，增加光合产物的积累，提高萱草的抗旱性。
- Abstract:
- Hemerocallis ‘Baltimore Oriole’ were used as test materials,using PEG-simulated drought stress,the effects of exogenous MeJA on the photosynthetic characteristics of Hemerocallis were studied, in order to improve the rational use of exogenous methyl jasmonate (MeJA) in the drought-resistant cultivation of Hemerocallis.The results showed that drought stress significantly reduced the day lilies photosynthetic pigment content and stomatal conductance (Gs),transpiration rate (Tr) and intercellular CO2 concentration (Ci),and improved the PSⅡ initial fluorescence (Fo),and the coefficient of photochemical quenching (NPQ),and the largest fluorescence (Fm),PSⅡ potential activity (Fv/Fo),maximum photochemical efficiency (Fv/Fm) and actual photochemical efficiency (Φ PSⅡ),coefficient of photochemical quenching (qP) were significantly suppressed,the final performance for lower photosynthetic rate (Pn).The exogenous MeJA significantly increased the photosynthetic pigment content under drought stress.To some extent,the damage to Hemerocallis was alleviated by reducing chlorophyll decomposition and improving the stability of photosynthetic mechanism in leaves.The Gs,Ci,Pn,Fm,Fv/Fo,Fv/Fm,ΦPSⅡ,qP of leaves increased,Fo and NPQ decreased,while jasmonate metabolic inhibitor (SHAM) treatment reversed the photosynthetic characteristics of MeJA leaves under drought stress.Alleviating the damage of photosynthetic mechanism and PSⅡ reaction center of Hemerocallis leaves under drought stress,improving the electron transfer rate and light energy conversion efficiency,thus enhancing the stomatal conductance and photosynthetic ability of leaves,increasing the accumulation of photosynthetic products and improving the drought resistance of Hemerocallis.
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备注/Memo
第一作者简介：曹冬梅（1971-），女，博士，研究员，现主要从事观赏植物逆境生理等研究工作。E-mail：caodm787@163.com.基金项目：山西省重点研发计划资助项目(201803D221005-6)；山西省深度贫困县科技精准扶贫专项资助项目(2019FP-15)。收稿日期：2020-11-26