[1]曹世睿,张婷,王其刚,等.八个蔷薇属种质资源的核型分析[J].北方园艺,2021,(13):85-90.[doi:10.11937/bfyy.20204689]
　CAO Shirui,ZHANG Ting,WANG Qigang,et al.Karyological Analysis on Eight Gemplasm Resources in Genus Rosa L.[J].Northern Horticulture,2021,(13):85-90.[doi:10.11937/bfyy.20204689]
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八个蔷薇属种质资源的核型分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年13 页码: 85-90 栏目: 出版日期: 2021-07-15
Title:
Karyological Analysis on Eight Gemplasm Resources in Genus Rosa L.
作者:
曹世睿1; 2; 张婷2; 王其刚2; 邱显钦2; 马长乐1; 蹇洪英2
（1.西南林业大学 园林园艺学院，云南 昆明 650224；2.云南省农业科学院 花卉研究所，国家观赏园艺工程技术研究中心，云南省花卉育种重点实验室，云南 昆明 650205）
Author(s):
CAO Shirui1; 2; ZHANG Ting2; WANG Qigang2; QIU Xianqin2; MA Changle1; JIAN Hongying2
(1.School of Landscape Architecture and Horticulture Science,Southwest Forestry University,Kunming,Yunnan 650224;2.Flower Research Institute,Yunnan Academy of Agricultural Sciences/National Engineering Research Center for Ornamental Horticulturae/Yunnan Key Laboratory for Flower Breeding,Kunming,Yunnan 650205)
关键词:
蔷薇属; 染色体; 倍性; 核型
Keywords:
Rosa L.; chromosome; ploidy; karyotype
DOI:
10.11937/bfyy.20204689
文献标志码:
A
摘要:
以亮叶月季等8个特异性状的蔷薇属种质资源为试材，采用去壁低渗法进行了染色体数目和核型研究，以期为现代月季品种进行种质创新和遗传改良提供细胞遗传学参考依据。结果表明：1）不同资源的染色体数目差异较大，半重瓣丽江蔷薇、亮叶月季、无刺的木香花、“苦水玫瑰”、黄刺玫为二倍体（2n=2x=14）；中国古老月季品种“淡黄香水月季”为三倍体（2n=3x=21）；食用玫瑰“滇红”为四倍体（2n=4x=28）；狗蔷薇为五倍体（2n=5x=35）；2）8个资源的核不对称系数变化范围在59.1%~66.9%，均未观察到随体的存在；3）重瓣丽江蔷薇、“淡黄香水月季”“滇红”、狗蔷薇的核型均为2B；重瓣木香花和黄刺玫的核型均为2A；“苦水玫瑰”的核型为1A；亮叶月季的核型为3A。
Abstract:
The chromosome numbers and karyotypes of eight rose germplasm resources with special traits were studied by de-walling and hypotonic methods.In order to supply cytogenetic information to the utilization of these rose resources for the germplasm innovation and cultivar improvement of modern rose.The results showed that,1) the chromosome numbers of different resources were significantly different.Rosa lichiangensis with half-double petals,Rosa lucidissima,the prickless Rosa banksiae Ait.var.banksiae,Rosa sertata×Rosa rugosa and Rosa xanthina f.xanthina were all diploid (2n=2x=14);Rosa ‘Parks’ Yellow Tea-scented China’ was triploid (2n=3x=21);Rosa‘Dian Hong’ was tetraploid (2n=4x=28).Rosa caninawas pentaploid (2n=5x=35).2) The range of nuclear asymmetry coefficient of these eight resources was 59.1%-66.9%,none satellite was observed in the metaphase nuclei of theseresources.3) The karyotypes of the half-double Rosa lichiangensis,Rosa‘Parks’ Yellow Tea-scented China’,Rosa ‘Dian Hong’,Rosa caninawere all 2B.Those of the prickless Rosa banksiae var.banksiae,Rosa xanthina f.xanthina were both 2A.The karyotype of Rosa sertata×Rosa rugosawas 1A and that of Rosa lucidissimawas 3A.

参考文献/References:

[1]JOYAUX F.History of roes incultivation European (Pre-1800)[J].Encyclopedia of Roseence,2003,20(2):395-402.[2]BEALES P.Classic roses:An illustrated encyclopedia and grower′s manual of old roses,shrub roses and climbers[M].London:Harvill Press,1997.[3]HORST R K.Compendium of rose diseases[M].Minnesota:The American Phytopathological Society,2003.[4]GUDIN S,Rose:Genetics and breeding[M].New York:John Wiley & Sons,Inc.,2000.[5]陈俊愉.中国花卉品种分类学[M].北京:中国林业出版社,2001.[6]LI J Z.Genetic and functional analysis of genes for black spot (Diplocarpon rosae) resistance in roses[D].Hannover:Leiblitz University,2007.[7]马燕,毛汉书,陈俊愉.部分月季花品种的数量分类研究[J].西北植物学报,1993(3):225-231.[8]张佐双,朱秀珍.中国月季[M].北京:中国林业出版社,2006.[9]STEBBINS G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971.[10]洪德元.植物细胞分类学[M].北京:科学出版社,1990.[11]李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996.[12]蹇洪英,张颢,李树发,等.蔷薇属植物的胞核学研究及其展望[J].西南农业学报,2009(1):207-211.[13]MA Y,ISLAM-FARIDI M N,CRANE C F,et al.In situ hybridization of ribosomal DNA to rose chromosomes[J].Journal of Heredity,1997,88(2):158-161.[14]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].植物科学学报,1985,3(4):297-302.[15]ARANO H.Cytological studies in subfamily carduoideae(Compositae) of Japan[J].Botanical Magazine of Tokyo,1963,76:32-39.[16]KUO S R,WANG T T,HUANG T C.Karyotype analysis of some for mosanagym nosperms[J].Taiwania,1972,17(1):66-80.[17]JIAN H Y,ZHANG T,WANG Q G,et al.Karyological diversity of wild Rosa in Yunnan,Southwestern China[J].Genetic Resources and Crop Evolution,2013,60:115-127.[18]蹇洪英,张颢,王其刚,等.中国古老月季品种的核型研究[J].园艺学报,2010,37(1):83-88.[19]刘承源,王辉,何亨辉,等.月季种质资源的倍性变异及核型多样性研究[J].西北植物学报,2015,35(4):707-715.[20]谭炯锐,王晶,高华北,等.中国17种蔷薇属植物45S和5S的rDNA分布研究[J].西北植物学报,2019(8):1333-1343.[21]惠俊爱,张霞,王绍明.新疆野生单叶蔷薇的染色体核型分析[J].山东林业科技,2013(4):58-60.[22]方桥,田敏,张婷,等.中甸刺玫及其近缘种基于FISH的核型分析[J].园艺学报,2020,47(3):100-113.[23]张婷,蹇洪英,王其刚,等.11个中国古老月季品种的核型分析[J].西南农业学报,2010,23(5):1656-1659.[24]罗乐,张启翔,白锦荣,等.16个中国传统月季品种的核型分析[J].北京林业大学学报,2009,31(5):90-95.[25]唐开学.云南蔷薇属种质资源研究[D].昆明:云南大学,2009.[26]JIAN H Y,TIAN M,ZHANG T,et al.Chromosome variation from Sect.Chinenses (Rosa L.) through Chinese old garden roses to modern rose cultivars[J].Acta Horticulturae,2013,977:157-165[27]马雪.部分蔷薇属植物核型分析及2n配子研究[D].哈尔滨:东北农业大学,2013.[28]刘佳.玫瑰与现代月季杂交育种及杂交亲和性研究[D].北京:北京林业大学,2013.[29]刘东华,李懋学.我国某些蔷薇属花卉的核型研究[J].植物科学学报,1985,3(4):403-408.[30]LIM K Y,WERLEMARK G,MATYASEK R,et al.Evolutionary implications of permanent odd polyploidy in the stable sexual,pentaploid of Rosa canina L.[J].Heredity,2005,94:501-506.[31]招雪晴,赵兰勇,张妮妮,等.‘重瓣玫瑰’和‘苦水玫瑰’的核型分析[J].山东林业科技,2007(5):50-51.

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备注/Memo

第一作者简介：曹世睿（1996-），女，硕士研究生，研究方向为月季种质资源细胞遗传学。E-mail:1114265848@qq.com.责任作者：蹇洪英（1976-），女，博士，研究员，现主要从事月季种质资源等研究工作。E-mail:ynwildflower@aliyun.com.基金项目：国家自然科学基金资助项目（31760087）；国家重点研发计划资助项目（2019YFD1000401-2）；云南省重大科技专项资助项目（生物医药）（202002AA100007）。收稿日期：2020-11-08

更新日期/Last Update: 2021-10-11