[1]高尚,曹小勇,胡选萍,等.四种重金属元素对白及种子萌发的影响[J].北方园艺,2021,(13):120-127.[doi:10.11937/bfyy.20203854]
　GAO Shang,CAO Xiaoyong,HU Xuanping,et al.Effects of Four Heavy Metals Elements on Seed Germination of Bletilla striata[J].Northern Horticulture,2021,(13):120-127.[doi:10.11937/bfyy.20203854]

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四种重金属元素对白及种子萌发的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年13 页码: 120-127 栏目: 出版日期: 2021-07-15

Title:: Effects of Four Heavy Metals Elements on Seed Germination of Bletilla striata

作者:: 高尚; 曹小勇; 胡选萍; 蒋景龙; 秦公伟; （陕西理工大学生物科学与工程学院，陕西省资源生物重点实验室，陕西汉中 723000）

Author(s):: GAO Shang; CAO Xiaoyong; HU Xuanping; JIANG Jinglong; QIN Gongwei; (College of Biological Science and Engineering,Shaanxi University of Technology/Shaanxi Key Laboratory of Bio-resource,Hanzhong,Shaanxi 723000)

关键词:: 白及; 种子; 重金属; 萌发

Keywords:: Bletilla striata; seed; heavy metal; germination

DOI:: 10.11937/bfyy.20203854

文献标志码:: A

摘要:: 以紫花白及种子为试材，去离子水作为对照，在重金属溶液中培养白及种子，研究了4种重金属（Cu、Cd、Pb、Hg）不同浓度（100、10、1、0.1、0.01 mg·L-1）对白及种子萌发的影响，以期探索白及种子对这4种重金属的耐受性，为重金属对兰科植物种子的萌发的研究提供参考依据。结果表明：光照下白及种子在去离子水中可以正常萌发，第10天萌发率达93%，20 d时形成叶片长为0.42 mm、原球茎直径为0.46 mm的自养性幼苗；4种重金属溶液均表现对白及种子萌发及幼苗生长的抑制作用；随着浓度的升高，抑制作用越明显；在高浓度（100 mg·L-1和10 mg·L-1）溶液中，除Pb外，其它3种重金属中（Cu、Cd、Hg）10 d和20 d的种子的萌发率均为0%；重金属对白及种子的萌发具有延迟效应；不同的重金属对种子萌发率及原球茎直径影响略有差异；在较低浓度（0.01 mg·L-1）溶液中，除Cd溶液20 d形成的原球茎直径略大于CK，约为0.53 mm，其它3种重金属均小于CK。

Abstract:: Bletilla striata seeds were used as materials,deionized water were used as control,and the method of cultivating Bletilla striataseeds in heavy metal solutions were used to study four kinds of heavy metals (Cu,Cd,Pb,Hg) with different concentrations (100 mg·L-1,10 mg·L-1,1 mg·L-1,0.1 mg·L-1,0.01 mg·L-1) on the germination of the seeds of Bletilla striata.In order to explore Bletilla striata seeds to four heavy metals tolerance and provide a reference for the study of the effects of heavy metals on the germination of other orchid seeds.The results showed that the seeds of Bletilla striata could germinate normally in deionized water under light.The germination rate reached 93% on the 10th day,and the autotrophic seedlings with a leaf length of 0.42 mm and a protocorm diameter of 0.46 mm were formed at 20 days;four kinds of heavy metal solution showed the inhibition to Bletilla striata seeds germination and growth;as the concentration increased,the inhibition became more obvious.In the high concentration (100 mg·L-1and 10 mg·L-1) solutions,except Pb,the other three heavy metals(Cu,Cd,Hg) germination rate of Bletilla striata seeds for 10 days and 20 days was 0%;heavy metals had delayed effects on the germination of Bletilla striata seeds;the effects of different heavy metals on seed growth were slightly different;in the lower concentration (0.01 mg·L-1) solution,the diameter of the protocorm formed in the solution except Cd for 20 days was slightly larger than CK,about 0.53 mm,and the other three heavy metals were smaller than CK.

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备注/Memo

第一作者简介：高尚（1996-），男，硕士研究生，研究方向为植物生物技术。E-mail:1271706450@qq.com.责任作者：曹小勇（1964-），男，硕士，教授，硕士生导师，现主要从事植物生物技术等研究工作。E-mail:caoxyong@163.com.基金项目：陕西省教育厅重点实验室科研计划资助项目（18JS017）。收稿日期：2020-09-08

更新日期/Last Update: 2021-10-11