ZHANG Baoqing,ZHAI Yaxin,XUE Lifang,et al.Biological Characteristic of Colletotrichum truncatum on Begonia semperflorens and Screening of Biocontrol Factors[J].Northern Horticulture,2021,(10):66-71.[doi:10.11937/bfyy.20202508]
四季秋海棠炭疽病菌生物学特性及生防因子的筛选
- Title:
- Biological Characteristic of Colletotrichum truncatum on Begonia semperflorens and Screening of Biocontrol Factors
- Keywords:
- Begonia semperflorens; Colletotrichum truncatum; biological characteristics; plant extracts; screening
- 文献标志码:
- A
- 摘要:
- 以四季秋海棠炭疽病菌（Colletotrichum truncatum）为试验材料，采用菌落生长法和孢子萌发法，研究了不同培养条件对四季秋海棠炭疽病菌菌落生长和孢子萌发的影响；采用生长速率法研究了14种植物提取物对四季秋海棠炭疽病菌的抑制作用，以期明确该病原菌生物学特性，筛选对其具有抑菌活性的植物提取物，为该病害的防治提供参考依据。结果表明：最适宜该病原菌菌丝生长的温度为25~30 ℃，pH 7，最适碳源为葡萄糖，最适氮源为硝酸钠和甘氨酸。分生孢子萌发的最适温度是30~35 ℃，pH 6~7。丁香、石菖蒲、蛇床子提取物在2.0 mg?mL-1浓度下对菌丝生长具有较好的抑制作用，菌丝生长抑制率分别为100%、92%和90%，其它植物提取物抑制率在20%~85%。
- Abstract:
- Colletotrichum truncatum causing anthracnose of Begonia semperflorens was used as experimental material.The effects of different temperatures，pH，carbon and nitrogen sources on the growth and spore germination of C.truncatum were determined by growth rate and spore germination methods.The inhibition of 14 kinds of plant extracts on C.truncatum were determined by growth rate method.Biological characteristics of the pathogen were studied and the plant extracts with antifungal activity were screened,in order to provide a reference for disease control.The results showed that the most suitable growth temperature for C.truncatum was 25-30 ℃ and pH 7.The optimum carbon source was glucose and the best nitrogen sources were sodium nitrate and glycine.The optimum temperature for conidia germination was 30-35 ℃ and pH 6-7.The inhibition rates of extract of Syzygium aromaticum，Acorus tatarinowii，Fructus cnidii at the concentration of 2.0 mg?mL-1 were 100%，92% and 90%，respectively.The inhibition rates of other plant extracts were between 20% and 85%.
参考文献/References:
[1]董莉娜,刘演,曹小燕.国产秋海棠属(秋海棠科)花粉形态及其分类学意义(英文)[J].广西植物,2016,36(1):73-82.[2]田代科,李春,肖艳,等.中国秋海棠属植物的自然杂交发生及其特点[J].生物多样性,2017,25(6):654-674.[3]杨亮.贵州秋海棠属的系统学研究[D].贵阳:贵阳中医学院,2009.[4]赵斌,付乃峰,向言词,等.光照强度对四季秋海棠及瓦氏秋海棠生长的影响[J].上海农业学报,2016,32(6):128-133.[5]周静波,黄成林,卜崇兴,等.“鸡尾酒”系列四季秋海棠栽培基质的筛选[J].安徽农业大学学报,2009,36(1):81-84.[6]田代科,李景秀,管开云,等.秋海棠属植物白粉病发生与防治[J].植物保护,2000(4):33-34.[7]阮义理.花卉灰霉病的综合防治[J].花木盆景(花卉园艺),2003(3):28-29.[8]田桂云.四季秋海棠常见病害及其防治[J].花木盆景(花卉园艺),2002(10):30.[9]魏宁生,吴云峰.花卉病毒病害的鉴定(Ⅰ)[J].云南农业大学学报,1988(1):26-32.[10]ZHAI Y X,ZHANG B Q,CAO H,et al.First report of Colletotrichum truncatum causing anthracnose of begonia in China[J].Plant Disease,2018,102(6):1177.[11]汪涛,钟荣顺,高智谋.大豆炭疽菌的营养生理研究[J].菌物研究,2006,4(3):91-93.[12]郑伟,王彬,彭丽娟,等.火龙果炭疽病病原鉴定与ITS序列分析[J].西南农业学报,2014,27(5):1970-1973.[13]陈国庆.中国柑橘炭疽病病原种类及种群遗传多样性研究[D].杭州:浙江大学,2010.[14]杨友联,刘永翔,刘作易.棉花炭疽病的病原鉴定[J].贵州农业科学,2014,42(8):86-91,95.[15]郑芳芳,贺伟,尹淑霞.20个苜蓿品种对炭疽病(Colletotrichum truncatum)的抗性测定[J].植物保护,2008(6):99-101.[16]苏琴.化学防治与生物防治的优缺点浅析[J].内蒙古农业科技,2011(6):84-85,132.[17]TORRES C C,TAPIA T R,HIGUERA C I,et al.Sensitivity of Colletotrichum truncatum to four fungicides and molecular characterization of thiabendazole-resistant isolates[J].Plant Disease,2015,99:1590-1595.[18]RAMDIAL H,HOSEIN F N,RAMPERSAD S N.Detection and molecular characterization of benzimidazole resistance among Colletotrichum truncatum isolates infecting bell pepper in Trinidad[J].Plant Disease,2016,100:1146-1152.[19]MIRESMAILI S,ISMAN M B.Botanical insecticides inspired by plant-herbivore chemical interactions[J].Trends in Plant Science,2014,19(1):29-35.[20]邵仁志,刘小安,孙兰,等.中国植物源农药的研究进展[J].湖北农业科学,2017,56(8):1401-1405.[21]杨再,陈佳铭,黄晓兰,等.天然植物有效成分的超声提取技术[J].饲料博览(技术版),2008(2):41-44.[22]沈静霆.砀山酥梨炭疽病菌生物学特性及有效药剂筛选[D].合肥:安徽农业大学,2012.[23]YE T,MA Z Q,WANG W Q,et al.Resistance induction of Fusarium graminearum to tebuconazole and bionomics of resistant-mutants (in Chinese)[J].Chinese Journal of Pesticide Science,2011,13(3):261-266.[24]孙志峰.大豆豆荚炭疽病的发病因子及其防治研究[D].杭州:浙江大学,2008.[25]刘铁秋,卢发瑞,杨晓红,等.石菖蒲醇提物对番茄灰霉抑菌稳定性及防效研究[J].天然产物研究与开发,2014,26(9):1368-1374,1420.[26]王红艳.丁香在植物保护中的应用[J].农业技术与装备,2015(3):52-53,56.[27]OSMANI Z,SIGHAMONY S.Effects of certain essential oils on mortality and metamorphosis of Aedes aegypti[J].Pesticides,1980,14(9):15-16.[28]王婷.丁香提取物对草莓保鲜效果的研究[D].沈阳:沈阳农业大学,2017.[29]闫训友,杜洪利,朱爱红,等.丁香提取物对鲜切鸭梨保鲜效应的研究[J].食品工业科技,2016,37(4):347-350,355.[30]郭新春.38种中药提取物对4种植物病原菌抑制活性的初步研究[D].长春:东北师范大学,2006.[31]邓业成,玉艳珍,王萌萌,等.石菖蒲提取物及其初步分离物的抑菌活性研究[J].安徽农业科学,2010,38(15):7836-7838.[32]姚英娟,徐雪亮,刘子荣,等.石菖蒲提取物对褐飞虱的作用方式研究[J].中国农学通报,2014,30(16):317-320.[33]何衍彪,詹儒林,赵艳龙.石菖蒲提取物对芒果炭疽菌及香蕉尖孢镰刀菌的抑制作用[J].云南农业大学学报,2006(6):728-732.[34]石志琦,沈寿国,徐朗莱,等.蛇床子素对植物病原真菌抑制机制的初步研究[J].农药学学报,2004(4):28-32.
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备注/Memo
第一作者简介：张宝清（1987-），女，硕士，政工师，现主要从事植物病原学等研究工作。E-mail：zbq1583505@163.com.责任作者：郝晓娟（1977-），女，博士，副教授，现主要从事植物病害生物防治等研究工作。E-mail：xiaojuanhao@126.com.基金项目：山西省重点研发计划资助项目（201803D221012-1）。收稿日期：2020-06-16