LI Qingzhu,CAI Youming,ZHANG Yongchun,et al.Research on Ornamental Traits and Hybridization Efficiency of Lycoris spp.Germplasm Resources[J].Northern Horticulture,2021,(05):56-64.[doi:10.11937/bfyy.20201181]
石蒜属资源观赏性状分析及杂交育种研究
- Title:
- Research on Ornamental Traits and Hybridization Efficiency of Lycoris spp.Germplasm Resources
- Keywords:
- Lycoris spp.; ornamental traits; ploidy; cross-breeding
- 文献标志码:
- A
- 摘要:
- 以18个石蒜属资源为试材，采用定期观测结合流式细胞仪倍性鉴定的方法，研究了不同石蒜属资源的主要观赏性状、倍性以及不同杂交组合对结实率的影响，以期为新的具有自主知识产权的石蒜品种的选育提供参考依据。结果表明：不同石蒜属资源在观赏性状上存在差异，花色主要有大红色、蓝紫色、白色、黄色等，部分资源如长筒石蒜和中国石蒜，花葶长达56.41~62.24 cm，花大健壮，是用作切花的良好材料。对石蒜属资源的倍性进行分析，鉴定到二倍体种质12份，三倍体种质6份。根据优势互补原则，设计了64个杂交组合，对杂交结果进行了统计，换锦花、中国石蒜、红蓝石蒜和长筒石蒜等资源具有良好的杂交结实率，是后续杂交育种优良亲本材料。
- Abstract:
- 18 Lycoris spp.resources were used as materials to study the ornamental characteristics,ploidy and the effects of hybrid combinations on seed setting rate by periodic observation and flow cytometry,in order to provide basis for the breeding of new varieties with independent intellectual property rights.The results showed that there were differences in ornamental characters among different Lycoris spp.resources.The flower colors included bright red,blue purple,white and yellow.Some resources,such as Lycoris longituba and Lycoris chinensis had 56.41-62.24 cm long scape and strong flowers,which were good materials for cut flowers.By analyzing the ploidy of Lycoris spp.resources,12 diploid and 6 triploid germplasm were identified.According to the principle of trait specificity and trait complementary,64 cross combinations were designed,and the results were statistically analyzed.The results showed that the Lycoris sprengeri,Lycoris chinensis,Lycoris haywardii and Lycoris longituba had good cross seed setting rate,which was a good parent material for subsequent cross breeding.
参考文献/References:
[1]令狐星慰,李多伟.石蒜属植物的研究进展综述[J].亚热带植物科学,2007,36(2):73-76.[2]郑玉,贾春,钱美华,等.石蒜属植物花形态建成研究进展[J].江苏农业科学,2012,40(10):172-175.[3]蔡军火,魏绪英,付振勇,等.石蒜属植物发育生物学与栽培技术研究进展[J].江西林业科技,2009(5):21-24.[4]袁菊红.中国石蒜属遗传多样性与亲缘关系的SSR分析[J].亚热带植物科学,2011,40(4):1-7.[5]孙长生,朱虹,彭志金.石蒜属植物加兰他敏含量研究[J].现代中药研究与实践,2011,25(6):30-31.[6]LIU X S,JIANG J,JIAO X Y,et al.Lycorine induces apoptosis and down-regulation of Mcl-1 in human leukemia cells[J].Cancer Letter,2009,274:16-24.[7]李青竹,杨柳燕,许俊旭,等.AHP层次分析法对上海石蒜属资源的综合评价[J].黑龙江农业科学,2019(8):22-25.[8]李淑顺,赵九洲,袁娥.几种石蒜属花卉观赏性状的灰色评价[J].徐州师范大学学报(自然科学版),2004(1):69-72.[9]蔡军火,魏绪英,李金峰,等.环境温度对红花石蒜生长节律的调控研究[J].江西农业大学学报,2018,40(1):24-31.[10]江生泉,姜自红,薛正帅.石蒜属植物栽培繁殖技术及园林应用研究[J].佛山科学技术学院学报(自然科学版),2017,35(2):76-79.[11]张鹏翀,鲍淳松,江燕.石蒜属植物生物学特性及栽培技术研究进展(综述)[J].亚热带植物科学,2015,44(2):168-174.[12]周日宝,王姣,李风娟,等.人工栽培黄花石蒜最适遮光度和最佳采收加工方法的研究[J].中药材,2014,37(11):1929-1931.[13]曾凡忠,杨永康,向极钎,等.忽地笑人工栽培技术[J].中国现代中药,2014,16(8):631-632.[14]蔡军火,魏绪英,张露.遮光对石蒜物质积累与切花品质的影响[J].草业科学,2013,30(9):1401-1406.[15]田春莲,覃翀,王鹏.石蒜属植物生物碱超声波-酶法协同提取及含量比较研究[J].中药材,2018,41(9):2167-2170.[16]李青竹,张永春,郑玉红,等.超高效液相色谱-四极杆线性离子阱质谱法同时检测石蒜属3种生物碱[J].分析测试学报,2018,37(2):211-216.[17]刘姚,麦任娣,黄晶,等.石蒜属植物生物碱类化合物及其构效关系研究进展[J].林业与环境科学,2019,35(5):114-121.[18]于海洋.中药石蒜生物碱类抗肿瘤活性研究[J].中国药理学与毒理学杂志,2019,33(9):728.[19]魏绪英,陈慧婷,李金峰,等.变温处理对红花石蒜鳞茎生理及开花的影响[J].江西农业大学学报,2019,41(6):1086-1092.[20]许俊旭,李青竹,杨柳燕,等.赤霉素和细胞分裂素对石蒜鳞茎膨大的调控作用及其生理机制[J].植物生理学报,2019,55(7):1029-1037.[21]李青竹,蔡友铭,杨贞,等.不同LED光质对石蒜幼苗生长、生理和生物碱积累的影响[J].应用与环境生物学报,2019,25(6):1414-1419.[22]张莹婷,杨秀莲,何岭,等.2种石蒜花芽分化与碳水化合物、抗氧化物酶及内源激素变化的关系[J].安徽农业大学学报,2019,46(2):342-349.[23]姜小凤.石蒜属植物杂交育种技术的研究[D].临安:浙江农林大学,2013.[24]时剑.石蒜属植物观赏新品种选育及繁育技术研究[D].临安:浙江农林大学,2011.[25]金梅.石蒜属植物种间杂交及后代培养的研究[D].南昌:江西农业大学,2013.[26]KURITA S.Variation and evolution on the karyotype of lycoris.Amaryllidacea II.Karyotype analyses of ten taxa among which seven are native to China[J].Cytologia,1987,52:19-40.[27]徐炳声,林巾箴,俞志洲,等.从花粉的生活力和杂交后的结实率评价石蒜属内的种间关系[J].遗传学报,1986(5):69-74.
相似文献/References:
[1]贺安娜,全妙华,欧立军,等.基于光合特性的石蒜属植物聚类分析[J].北方园艺,2013,37(13):22.
HE An-na,QUAN Miao-hua,OU Li-jun,et al.Cluster Analysis of Lycoris Herb.Based on Photosynthetic Characteristics[J].Northern Horticulture,2013,37(05):22.
[2]刘合霞,周 坚.石蒜愈伤组织的诱导及其植株再生研究[J].北方园艺,2013,37(06):93.
LIU He-xia,ZHOU Jian.Study on Callus Induction and Plant Regeneration of Lycoris radiate Herb.[J].Northern Horticulture,2013,37(05):93.
[3]秦 公 伟,等.石蒜不同器官酯酶和苹果酸脱氢酶同工酶酶谱分析[J].北方园艺,2010,34(11):0.[doi:10.11937/bfyy.2010011050]
QIN Gong -wei,CAO Xiao -yong,GENG Jing -zhang,et al.Zymogram of Esterase and Malate Dehydrogenase in Different Organs of Lycoris radiate ( L’ Her) Herb.[J].Northern Horticulture,2010,34(05):0.[doi:10.11937/bfyy.2010011050]
[4]董仲国,国淑梅,张艳君,等.十一个北美海棠品种在山东地区的生长特性及观赏性状研究[J].北方园艺,2016,40(05):76.[doi:10.11937/bfyy.201605022]
DONG Zhongguo,GUO Shumei,ZHANG Yanjun,et al.tudy on the Growth Characteristics and Ornamental Characters of 11 Varieties of North American Begonia in Shandong Region[J].Northern Horticulture,2016,40(05):76.[doi:10.11937/bfyy.201605022]
[5]秦 公 伟,等.两种石蒜中酯酶和苹果酸脱氢酶酶谱分析[J].北方园艺,2009,33(02):0.[doi:10.11937/bfyy.200902033]
QIN Gong -wei,CAO Xiao -yong,et al.Analysis of Esterase and Malate Dehydrogenase on T wo Different Amaryllis[J].Northern Horticulture,2009,33(05):0.[doi:10.11937/bfyy.200902033]
[6]尹娟,蔡秀珍,田径.野生花卉资源调查及其观赏性状的评价[J].北方园艺,2017,41(17):119.[doi:10.11937/bfyy.20170181]
YIN Juan,CAI Xiuzhen,TIAN Jing.Germplasm Resources of Wild Flower and Ornamental Characteristics Evaluation[J].Northern Horticulture,2017,41(05):119.[doi:10.11937/bfyy.20170181]
[7]杨柳燕,李青竹,蔡友铭,等.二十四个朱顶红品种观赏性状分析及杂交育种研究[J].北方园艺,2019,43(01):109.[doi:10.11937/bfyy.20181962]
YANG Liuyan,LI Qingzhu,CAI Youming,et al.Research on Ornamental Traits and Hybridization Efficiency of Twenty-four Hippeatrum Cultivars[J].Northern Horticulture,2019,43(05):109.[doi:10.11937/bfyy.20181962]
[8]徐亚萍,贺菡莹,赵鑫,等.B9和PP333对菊花‘Mona Lisa Sunny’观赏性状和生理指标的影响[J].北方园艺,2021,(05):69.[doi:10.11937/bfyy.20201354]
XU Yaping,HE Hanying,ZHAO Xin,et al.Effects of B9 and PP333 on Ornamental Characters and Physiological Indexes of Chrysanthemum ‘Mona Lisa sunny’[J].Northern Horticulture,2021,(05):69.[doi:10.11937/bfyy.20201354]
备注/Memo
第一作者简介：李青竹（1986-），女，博士，副研究员，现主要从事花卉遗传育种与代谢调控等研究工作。E-mail:1984yu1986@163.com.责任作者：蔡友铭（1965-），男，博士，研究员，现主要从事花卉遗传育种与产业化开发利用等研究工作。E-mail:saascym1@163.com.基金项目：上海市科技兴农重点攻关资助项目(沪农科攻字(2016)第6-1-6号)。收稿日期：2020-03-25