FENG Shasha,FAN Cuili,ZHENG Zhixin,et al.Induction and Differentiation of Trollius chinensis Bunge.[J].Northern Horticulture,2021,(04):107-112.[doi:10.11937/bfyy.20201054]
金莲花愈伤组织诱导与分化
- Title:
- Induction and Differentiation of Trollius chinensis Bunge.
- Keywords:
- Trollius chinensis Bunge.; callus; induction; differentiation
- 文献标志码:
- A
- 摘要:
- 以金莲花无菌种子苗为试材，采用组织培养的方法，研究了外植体种类、基本培养基和外源激素等对愈伤组织诱导和不定芽再生的影响，以期建立其快速繁殖体系。结果表明：金莲花无菌种子苗的子叶为最佳外植体；MS是诱导愈伤组织的最佳基本培养基；附加6-BA和2,4-D的浓度分别为2.0 mg?L-1和1.0 mg?L-1时，愈伤组织的生长量最大，达到72.00%，长势最好；30 g?L-1芽麦糖作为碳源优于蔗糖；培养基添加75 mg?L-1的活性炭可有效的抑制褐化现象；金莲花愈伤组织分化的最佳附加植物生长调节剂为NAA 0.5 mg?L-1和KT 1.0 mg?L-1。
- Abstract:
- Taking the seedlings of Trollius chinensis Bunge.in vitro as test materials，the effects of explant type，basic medium and exogenous hormone on callus induction and adventitious bud regeneration were studied by tissue culture method in order to establish its rapid propagation system.The results showed that the optimal explants were cotyledons from seedlings of Trollius chinensis Bunge.MS medium was the best medium for callus induction.Medium plus 2.0 mg?L-1 6-BA and 1.0 mg?L-1 2，4-D was advantageous to callus induction，and callus rate was 72.00%.Maltose 30 g?L-1 as carbon source was better than sucrose.The medium adding activated charcoal 75 mg?L-1 could prevent brown efficiently.The differentiation medium adding 0.5 mg?L-1 NAA and 1.0 mg?L-1 KT，could improve the differentiation rate of callus.
参考文献/References:
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备注/Memo
第一作者简介：冯莎莎（1981-），女，硕士，讲师，现主要从事园艺植物生物技术等研究工作。E-mail:fengshasha@yeah.net.责任作者：孙洪生（1961-），男，博士，教授，现主要从事中医基础理论等研究工作。E-mail：sun003@126.com.基金项目：河北省重点研发资助项目（18226316D）；河北北方学院校级重大资助项目（ZD201411）；河北省高等学校科学技术研究青年基金资助项目（QN2015165）。收稿日期：2020-08-28