BAI Xiaoxue,QIN Hongyan,HAN Xianyan,et al.Preliminary Research on Sterilization Method of Actinidia arguta Buds for Cryopreservation[J].Northern Horticulture,2020,44(24):45-50.[doi:10.11937/bfyy.20200934]
用于超低温保存的软枣猕猴桃枝芽灭菌方法初探
- Title:
- Preliminary Research on Sterilization Method of Actinidia arguta Buds for Cryopreservation
- 文献标志码:
- A
- 摘要:
- 以软枣猕猴桃休眠枝条与当年生新梢茎段为试材，采用单因素试验方法，以期通过对软枣猕猴桃枝条外植体及其芽进行严格灭菌，确保利用软枣猕猴桃休眠芽及新梢腋芽进行超低温保存后复苏的成活率。结果表明：休眠期单芽茎段以去皮后再采用0.1% HgCl2灭菌30 min为宜；当年生新梢单芽茎段以采用0.1% HgCl2灭菌10 min为宜。
- Abstract:
- Dormant branches and the one-year-old stem segments of Actinidia argutawere taken as the test materials,a single factor test method was used to strictly sterilize the explants and buds of Actinidia arguta to ensure the survival rate of dormant buds and new shoots after cryopreservation.The results showed that the single-bud stem segment in the dormant period was sterilized by 0.1% HgCl2 for 30 minutes after peeling;the single-bud stem segment of the new shoot was sterilized by 0.1% HgCl2 for 10 minutes.
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备注/Memo
第一作者简介：白晓雪（1995-），女，硕士研究生，研究方向为果树种质资源。E-mail：2429239371@qq.com.责任作者：李昌禹（1971-），男，硕士，研究员，现主要从事果树种质资源等研究工作。E-mail：lichangyu@caas.cn.基金项目：吉林省科技发展计划资助项目（20170203006NY）。收稿日期：2020-03-13